Formation of double emulsion micro-droplets in a microfluidic device using a partially hydrophilic–hydrophobic surface

Kamnerdsook, Ampol; Juntasaro, Ekachai; Khemthongcharoen, Numfon; Chanasakulniyom, Mayuree; Sripumkhai, Witsaroot; Pattamang, Pattaraluck; Promptmas, Chamras; Atthi, ์์Nithi; Jeamsaksiri, Wutthinan

doi:10.1039/d1ra06887c

Cited by 12 publications

(13 citation statements)

References 52 publications

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“…Similar approaches using microfluidic double emulsions [40][41][42]50 or W/O microfluidic droplet selection systems (FADS) 25 have been reported for the identification of microorganisms with bactericidal activity. However, to produce double emulsions, partially hydrophilic-hydrophobic microfluidic devices are needed, 28 as well as the introduction of flow resistance elements to achieve a single aqueous core emulsion. 51 On the other hand, sorting particles with FADS has only been demonstrated with at most two different fluorescent parameters, 52 which is less advantageous than the combinations of scattering and fluorescence signals offered by FACS.…”

Section: Discussionmentioning

confidence: 99%

“…To address this limitation, gel beads 26,27 or double emulsions water-oil-water (W/O/W) droplets 28,29 formed in microfluidic devices can be exploited as substitutes for W/O droplets. These compartments can be easily removed from the continuous oil phase and transferred to an aqueous phase for further analysis and sorting using Fluorescence Activated Cell Sorting (FACS), a more accessible technique.…”

Section: Introductionmentioning

confidence: 99%

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High-throughput bacterial co-encapsulation in microfluidic gel beads for discovery of antibiotic-producing strains

Ochoa,

Gastélum,

Rocha

et al. 2023

Analyst

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High-throughput bacterial co-encapsulation in microfluidic gel beads for discovery of antibiotic-producing strains

Ochoa,

Gastélum,

Rocha

et al. 2023

Analyst

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“…by simply regulating the channel shape, solvent type, solidification methods, flow rates, types, concentration of spinning solution, etc. [ 133 ].…”

Section: Microfluidic Spinning Technology For Micro-/nanofibers and F...mentioning

confidence: 99%

Recent Trends of Microfluidics in Food Science and Technology: Fabrications and Applications

Pang

et al. 2022

Foods

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The development of novel materials with microstructures is now a trend in food science and technology. These microscale materials may be applied across all steps in food manufacturing, from raw materials to the final food products, as well as in the packaging, transport, and storage processes. Microfluidics is an advanced technology for controlling fluids in a microscale channel (1~100 μm), which integrates engineering, physics, chemistry, nanotechnology, etc. This technology allows unit operations to occur in devices that are closer in size to the expected structural elements. Therefore, microfluidics is considered a promising technology to develop micro/nanostructures for delivery purposes to improve the quality and safety of foods. This review concentrates on the recent developments of microfluidic systems and their novel applications in food science and technology, including microfibers/films via microfluidic spinning technology for food packaging, droplet microfluidics for food micro-/nanoemulsifications and encapsulations, etc.

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“…We developed a straightforward chip-capillary setup, which allowed us to generate regularly spaced DEDs of the o 1 /w/o 2 type in a two-step emulsification process and to couple them for the first time with ESI-MS. The generation of DEDs in a two-step emulsification process in microfluidic chips or capillary devices is a standard procedure [39][40][41]. However, many works focus on the fabrication of w 1 /o/w 2 DEDs with thin or even ultrathin shells due to increased stabilities of the droplets, and relatively high surfactant concentrations (often between 1-10 wt%) are utilized [28,33,[42][43][44][45][46].…”

Section: Development Of a Chip-capillary Setup To Generate Double-emu...mentioning

confidence: 99%

Analysis of double-emulsion droplets with ESI mass spectrometry for monitoring lipase-catalyzed ester hydrolysis at nanoliter scale

et al. 2022

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Microfluidic double-emulsion droplets allow the realization and study of biphasic chemical processes such as chemical reactions or extractions on the nanoliter scale. Double emulsions of the rare type (o1/w/o2) are used here to realize a lipase-catalyzed reaction in the non-polar phase. The surrounding aqueous phase induces the transfer of the hydrophilic product from the core oil phase, allowing on-the-fly MS analysis in single double droplets. A microfluidic two-step emulsification process is developed to generate the (o1/w/o2) double-emulsion droplets. In this first example of microfluidic double-emulsion MS coupling, we show in proof-of-concept experiments that the chemical composition of the water layer can be read online using ESI–MS. Double-emulsion droplets were further employed as two-phase micro-reactors for the hydrolysis of the lipophilic ester p-nitrophenyl palmitate catalyzed by the Candida antarctica lipase B (CalB). Finally, the formation of the hydrophilic reaction product p-nitrophenol within the double-emulsion droplet micro-reactors is verified by subjecting the double-emulsion droplets to online ESI–MS analysis. Graphical abstract

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Formation of double emulsion micro-droplets in a microfluidic device using a partially hydrophilic–hydrophobic surface

Abstract: (a) Droplet encapsulation efficiency & inner and outer diameters of water-in-oil-in-water droplets at various frequency ratios and flow rate ratios and (b) Images of water-in-oil-in-water droplets over a frequency-ratio range of fr = 0.73–1.30

Cited by 12 publications

References 52 publications

High-throughput bacterial co-encapsulation in microfluidic gel beads for discovery of antibiotic-producing strains

High-throughput bacterial co-encapsulation in microfluidic gel beads for discovery of antibiotic-producing strains

Recent Trends of Microfluidics in Food Science and Technology: Fabrications and Applications

Analysis of double-emulsion droplets with ESI mass spectrometry for monitoring lipase-catalyzed ester hydrolysis at nanoliter scale

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