Formation of colonies of fibroblasts in cultures of circulating blood cells

Panasyuk, A. F.; Luriya, E. A.

doi:10.1007/bf00845244

Cited by 2 publications

(2 citation statements)

References 3 publications

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“…When studying connective tissue transformation of blood cells, the major concern has been the possibility of contaminating blood samples with cells from the blood vessel or heart walls [10, 13, 42, , –45]. An important, although not definitive, result was demonstration that the concentration of adherent colony‐forming cells in guinea pig blood was independent of the number of cardiac punctures used to obtain the blood [46, 47]. The cannulation technique has been widely accepted as the safest way of avoiding contamination, even more so when accompanied by discarding the first portion of blood [13, 42, 45].…”

Section: Discussionmentioning

confidence: 99%

Circulating Connective Tissue Precursors: Extreme Rarity in Humans and Chondrogenic Potential in Guinea Pigs

et al. 2007

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Using a variety of cell separation techniques and cultivation conditions, circulating, adherent, connective tissue, clonogenic cells were found in just 3 donors out of 66, demonstrating that these precursors are extremely rare in postnatal human blood. Contrary to humans, guinea pig blood shows much more reproducible connective tissue colony formation; it was therefore chosen to study the differentiation potential of adherent blood-derived clonogenic cells. Out of 22 single colony-derived strains of various morphologies, only 5 spindle-shaped strains showed extensive proliferative capacity in vitro. None of these strains formed bone upon in vivo transplantation, whereas two strains formed cartilage in high-density pellet cultures in vitro. Both chondrogenic strains included cells expressing aggrecan, whereas nonchondrogenic strains did not. Out of four polyclonal strains studied, one formed both cartilage and abundant bone accompanied by hematopoiesis-supporting stroma. Evidently, there are cells in adult guinea pig blood capable of both extensive proliferation and differentiation toward cartilage: circulating chondrogenic precursors. Although some of these cells lack osteogenic potential and therefore represent committed chondrogenic precursors, others may be multipotential and consequently belong to the family of skeletal stem cells. This is the first demonstration of postnatal circulating chondrogenic precursors, as well as of precursor cells with chondrogenic but not osteogenic potential.

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Section: Discussionmentioning

confidence: 99%

Circulating Connective Tissue Precursors: Extreme Rarity in Humans and Chondrogenic Potential in Guinea Pigs

et al. 2007

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“… 4 MSCs were found in all studied tissues (including peripheral blood) of adult animals belonging to various species. 23 , 24 , 25 Due to ability to accept unusual phenotype termed plasticity, 5 immunomodulating ability 26 , 27 and secretion of humoral factors activating endogenous mechanisms of regeneration, MSCs became a valuable new source for cell therapy. 28 , 29 MSCs show efficacy in cell therapy of variety of degenerative, inflammatory, traumatic, and immune diseases of various organs.…”

Section: Mesenchymal Stem Cells As a Type Of Tissue Specific Adult Stmentioning

confidence: 99%

pRb-E2F signaling in life of mesenchymal stem cells: Cell cycle, cell fate, and cell differentiation

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2014

Genes & Diseases

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Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into various mesodermal lines forming fat, muscle, bone, and other lineages of connective tissue. MSCs possess plasticity and under special metabolic conditions may transform into cells of unusual phenotypes originating from ecto- and endoderm. After transplantation, MSCs release the humoral factors promoting regeneration of the damaged tissue. During last five years, the numbers of registered clinical trials of MSCs have increased about 10 folds. This gives evidence that MSCs present a new promising resource for cell therapy of the most dangerous diseases. The efficacy of the MSCs therapy is limited by low possibilities to regulate their conversion into cells of damaged tissues that is implemented by the pRb-E2F signaling. The widely accepted viewpoint addresses pRb as ubiquitous regulator of cell cycle and tumor suppressor. However, current publications suggest that basic function of the pRb-E2F signaling in development is to regulate cell fate and differentiation. Through facultative and constitutive chromatin modifications, pRb-E2F signaling promotes transient and stable cells quiescence, cell fate choice to differentiate, to senesce, or to die. Loss of pRb is associated with cancer cell fate. pRb regulates cell fate by retaining quiescence of one cell population in favor of commitment of another or by suppression of genes of different cell phenotype. pRb is the founder member of the “pocket protein” family possessing functional redundancy. Critical increase in the efficacy of the MSCs based cell therapy will depend on precise understanding of various aspects of the pRb-E2F signaling.

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Formation of colonies of fibroblasts in cultures of circulating blood cells

Cited by 2 publications

References 3 publications

Circulating Connective Tissue Precursors: Extreme Rarity in Humans and Chondrogenic Potential in Guinea Pigs

Circulating Connective Tissue Precursors: Extreme Rarity in Humans and Chondrogenic Potential in Guinea Pigs

pRb-E2F signaling in life of mesenchymal stem cells: Cell cycle, cell fate, and cell differentiation

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