Formation of cholinergic synapses between dissociated sympathetic neurons and skeletal myotubes of the rat in cell culture.

Nurse, Colin A.; O'Lague, Paul H.

doi:10.1073/pnas.72.5.1955

Cited by 79 publications

(17 citation statements)

References 46 publications

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“…However, conventional parasympathetic neurones do not depend on NGF for their maintenance in vivo or in vitro (Levi-Montalcini & Angeletti, 1968;Helfand, Riopelle & Wessels, 1978). In addition, authentic sympathetic neurones cultured with muscle form abundant cholinergic synapses with each other (O'Lague, Macleish, Nurse, Claude, Furshpan & Potter, 1975;Nurse, 1977); nodose neurones in co-culture with muscle form few cholinergic synapses (Baccaglini & Cooper, 1981). Therefore, tentatively we conclude that the cholinergic and cholinoceptive nodose neurones in culture would have developed into sensory neurones if they had been left in vivo.…”

Section: Discussionmentioning

confidence: 91%

Electrophysiological studies of new‐born rat nodose neurones in cell culture.

Baccaglini¹,

Cooper²

1982

The Journal of Physiology

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SUMMARY1. Neurones of the nodose ganglion of the vagus nerve were dissociated from new-born rats and grown in the virtual absence of non-neuronal cells and in the presence of nerve growth factor.2. The resting potentials of the neurones ranged from -40 to -80 mV. Action potentials were of short duration, with no inflexion on the falling phase; others were of longer duration with a hump on the falling phase.3. The inward current of the action potential was carried either predominantly by Na+ or by Na+ and Ca2+.4. Tetrodotoxin (1 PM) blocked the Na+ channels of some neurones but in other neurones the Na+ channels were partially or completely resistant to tetrodotoxin (1-10 /M).5. Many neurones formed excitatory synapses on neighbouring neurones which were blocked or greatly reduced by conventional ganglionic nicotinic antagonists. This indicates that these neurones secreted ACh and expressed ACh receptors at these synapses.6. The accompanying paper (Baccaglini & Cooper, 1981) reports the effect of co-culturing nodose neurones with non-neuronal cells on the expression of functional nicotinic receptors.

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Section: Discussionmentioning

confidence: 91%

Electrophysiological studies of new‐born rat nodose neurones in cell culture.

Baccaglini¹,

Cooper²

1982

The Journal of Physiology

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“…Thus, a-Bgt is a potentially useful marker f o r the goldfish brain nicotinic acetylcholine receptor (nAcChR). Whereas a-Bgt blocks cholinergic agonist-induced responses in goldfish optic tectum, it appears to be ineffective in blocking acetylcholine responses in most ganglionic neurons (Chou and Lee, 1969;Nurse and O'Lague, 1975;Patrick and Stallcup, 1977u,b;Brown and Fumagalli, 1977; Carbonetto et al, 1978; Kouvelas et al, 1978; Ravdin and Berg, 1979). These results suggest that the a-Bgt binding protein of goldfish optic tectum may be more closely related to that from muscle and electroplaque than that from ganglia (Oswald and Freeman, 1981).…”

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confidence: 84%

Analysis of α‐Bungarotoxin Binding in the Goldfish Central Nervous System

Oswald

Freeman

1981

Journal of Neurochemistry

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We report here the equilibrium, kinetic, and pharmacological analysis of alpha-125I-bungarotoxin (alpha-125I-Bgt) binding to a Triton X-100-solubilized goldfish brain synaptosomal fraction. In addition, a refined analysis of equilibrium binding to a particulate synaptosomal fraction is presented. Equilibrium binding from both particulate and soluble fractions revealed an apparent heterogeneity of binding sites. Kinetic analysis of the soluble receptor revealed linear association kinetics and nonlinear dissociation kinetics. The dissociation curve suggested the presence of at least two rate constants. Potential sources of the binding heterogeneity found in both the equilibrium binding and dissociation kinetics experiments are (1) multiple receptor species, (2) multiple ligand species, and (3) different, possibly interconvertible, states of a single receptor type. No evidence for the first two alternatives was found. Support for the third alternative was obtained by observing the effect of cholinergic ligands on alpha-125I-Bgt dissociation. Carbamylcholine and d-tubocurarine increased the apparent proportion of rapidly dissociating sites, suggesting that the two binding affinities can be interconverted and may arise from a single receptor type. Evidence concerning the identity of the alpha-Bgt binding protein as a nicotinic acetylcholine receptor is discussed.

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“…It is also interesting to note that the action of DTOX 6 (40 pg/ml) on VR-DRP was not blocked by pretreatment of the preparations with a-bungarotoxin (a-BGT) (25 pg/ml). This is not surprising considering that ct-BGT does not block nicotinic receptors at neuronal synapses, unlike its action at the neuromuscular junctien (Chou & Lee, 1969;Miledi & Potter, 1971;Nurse & O'Lague, 1975;Miledi & Szczepaniak, 1975;Duggan, Hall & Lee, 1976 1978; Kouvelas, Dichter & Greene, 1978;Morley, Kemp & Salvaterra, 1979;Patrick et al, 1980; but see also Chiappinelli & Zigmond, 1978;Fex & Adams, 1978;Marshall, 1979).…”

Section: (F) Protection Experimentsmentioning

confidence: 99%

Convulsant and Possible Anticholinergic Actions of Dendrotoxin in the Amphibian Spinal Cord

Collier

Padjen

Quik

et al. 1981

British J Pharmacology

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1 Dendrotoxin (DTOX)6, 6a and 5,6-1, fractions of the venom isolated from the green mamba (Dendroaspis viridis) promoted both spontaneous and stimulus-coupled rhythmic activity and antagonized the cholinergically mediated ventral root-dorsal root potential (VR-DRP) of frog spinal cord. The different time course and reversibility of these two effects indicates that the toxin has two entirely separate sites of action on the frog spinal cord. 2 Since DTOX 6 neither blocked nor enhanced responses of ventral and dorsal roots to glutamate, -y-aminobutyric acid (GABA), ,3-alanine, glycine or aspartate, it is unlikely that its convulsant action resulted from an alteration of the postsynaptic actions of inhibitory or excitatory amino acids. 3 An alteration in the threshold for action potential generation could perhaps contribute to the convulsant action of DTOX 6, although other mechanisms such as blockade of the release of unspecified inhibitory substances cannot be excluded. 4 In addition to the lack of effect on amino acid responses, DTOX failed to block the polysynaptic DR-VRP or DR-DRP pathways, which are mediated at least in part by amino acid neurotransmitters. Although this would be consistent with a specific action of DTOX at the cholinergic synapse of the VR-DRP pathway, this site of action has not yet been demonstrated unequivocally. Other possible mechanisms whereby DTOX could block VR-DRP are discussed.

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Formation of cholinergic synapses between dissociated sympathetic neurons and skeletal myotubes of the rat in cell culture.

Cited by 79 publications

References 46 publications

Electrophysiological studies of new‐born rat nodose neurones in cell culture.

Electrophysiological studies of new‐born rat nodose neurones in cell culture.

Analysis of α‐Bungarotoxin Binding in the Goldfish Central Nervous System

Convulsant and Possible Anticholinergic Actions of Dendrotoxin in the Amphibian Spinal Cord

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