1975
DOI: 10.1073/pnas.72.5.1955
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Formation of cholinergic synapses between dissociated sympathetic neurons and skeletal myotubes of the rat in cell culture.

Abstract: Sympathetic principal neurons, dissociated from superior cervical ganglia of newborn rats, were plated into cultures containing rat skeletal myotubes formed from previously plated primary myoblasts. Electrophysiological evidence is presented that the neurons developed cholinergic synapses with the myotubes. In addition, the neurons developed cholinergic synapses with each other as previously reported [O'Lague et al. (1974) Proc. Nat. Acad. Sci. USA 71, 3602-36061. The

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Cited by 79 publications
(17 citation statements)
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“…However, conventional parasympathetic neurones do not depend on NGF for their maintenance in vivo or in vitro (Levi-Montalcini & Angeletti, 1968;Helfand, Riopelle & Wessels, 1978). In addition, authentic sympathetic neurones cultured with muscle form abundant cholinergic synapses with each other (O'Lague, Macleish, Nurse, Claude, Furshpan & Potter, 1975;Nurse, 1977); nodose neurones in co-culture with muscle form few cholinergic synapses (Baccaglini & Cooper, 1981). Therefore, tentatively we conclude that the cholinergic and cholinoceptive nodose neurones in culture would have developed into sensory neurones if they had been left in vivo.…”
Section: Discussionmentioning
confidence: 91%
“…However, conventional parasympathetic neurones do not depend on NGF for their maintenance in vivo or in vitro (Levi-Montalcini & Angeletti, 1968;Helfand, Riopelle & Wessels, 1978). In addition, authentic sympathetic neurones cultured with muscle form abundant cholinergic synapses with each other (O'Lague, Macleish, Nurse, Claude, Furshpan & Potter, 1975;Nurse, 1977); nodose neurones in co-culture with muscle form few cholinergic synapses (Baccaglini & Cooper, 1981). Therefore, tentatively we conclude that the cholinergic and cholinoceptive nodose neurones in culture would have developed into sensory neurones if they had been left in vivo.…”
Section: Discussionmentioning
confidence: 91%
“…Thus, a-Bgt is a potentially useful marker f o r the goldfish brain nicotinic acetylcholine receptor (nAcChR). Whereas a-Bgt blocks cholinergic agonist-induced responses in goldfish optic tectum, it appears to be ineffective in blocking acetylcholine responses in most ganglionic neurons (Chou and Lee, 1969;Nurse and O'Lague, 1975;Patrick and Stallcup, 1977u,b;Brown and Fumagalli, 1977; Carbonetto et al, 1978; Kouvelas et al, 1978; Ravdin and Berg, 1979). These results suggest that the a-Bgt binding protein of goldfish optic tectum may be more closely related to that from muscle and electroplaque than that from ganglia (Oswald and Freeman, 1981).…”
mentioning
confidence: 84%
“…It is also interesting to note that the action of DTOX 6 (40 pg/ml) on VR-DRP was not blocked by pretreatment of the preparations with a-bungarotoxin (a-BGT) (25 pg/ml). This is not surprising considering that ct-BGT does not block nicotinic receptors at neuronal synapses, unlike its action at the neuromuscular junctien (Chou & Lee, 1969;Miledi & Potter, 1971;Nurse & O'Lague, 1975;Miledi & Szczepaniak, 1975;Duggan, Hall & Lee, 1976 1978; Kouvelas, Dichter & Greene, 1978;Morley, Kemp & Salvaterra, 1979;Patrick et al, 1980; but see also Chiappinelli & Zigmond, 1978;Fex & Adams, 1978;Marshall, 1979).…”
Section: (F) Protection Experimentsmentioning
confidence: 99%