Formation of Aminosilane-Functionalized Mica for Atomic Force Microscopy Imaging of DNA

Crampton, Neal; Bonass, William A.; Kirkham, Jennifer; Thomson, Neil H.

doi:10.1021/la050972q

Cited by 82 publications

(106 citation statements)

References 34 publications

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“…Silanization of solid surfaces with APTES is widely used to prepare substrates for applications using immobilized proteins and to prepare selective absorbents or organic/inorganic hybrid materials (Kurth and Bein, 1995;Hu et al ., 1996;Frey et al ., 1997;Joos et al ., 1997;Szabo et al ., 1998;Gyorvary et al ., 1999;Falsey et al ., 2001;LeProust et al ., 2001;Kim and Abbott, 2002;Matsumoto et al ., 2002;Ek et al ., 2003;Etienne and Walcarius, 2003;Jin et al ., 2003;Miyazaki et al ., 2003;Vaidya and Norton, 2004;Crampton et al ., 2005;Chen et al ., 2006;Enders et al ., 2007;Martin et al ., 2007;Yu et al ., 2007). Chemically adsorbed silane on an inorganic surface provides a platform for further chemical reactions through an amine or other functional group opposite to the silane.…”

Section: Introductionmentioning

confidence: 99%

“…Macroscale properties of silane films have been characterized by use of Fourier transform infrared (FTIR) spectroscopy (Kessel and Granick, 1991;Kanan et al ., 2002), X-ray photoelectron spectroscopy (Moon et al ., 1997;Allen et al ., 2005;Crampton et al ., 2005;Chen et al ., 2006;Metwalli et al ., 2006;Martin et al ., 2007), UV-vis spectrophotometry (Moon et al ., 1996(Moon et al ., , 1997, ellipsometry (Kurth and Bein, 1995;Moon et al ., 1996Moon et al ., , 1997, and quartz-crystal microbalance measurements (Kurth and Bein, 1995). These methods have provided essential insight into film properties but cannot describe the distribution of available amino groups or the microscale morphology of the film.…”

Section: Introductionmentioning

confidence: 99%

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Morphology and Amine Accessibility of (3‐Aminopropyl) Triethoxysilane Films on Glass Surfaces

Wang

Vaughn

2008

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3-Aminopropyl) triethoxysilane (APTES) is commonly used to functionalize glass substrates because it can form an amine-reactive film that is tightly attached to the surface. In this study, we investigated the morphology and chemical reactivity of APTES films prepared on glass substrates using common deposition techniques. Films were prepared using concentrated vapor-phase deposition, dilute vapor-phase deposition, anhydrous organic-phase deposition and aqueous-phase deposition. All films were annealed, or cured, at 150 degrees C. The morphology of the films was quantified by fluorescence and by atomic force microscopy (AFM). The optical equivalent of the AFM images was computed and then used to directly compare optical and AFM images. Reactive amine density was determined by a picric acid assay and by a method that employed N-succinimidyl 3-[2-pyridyldithio]-propionamido (SPDP) cross-linked rhodamine. Fluorescence and AFM images showed that silane films prepared from dilute vapor-phase and aqueous-phase deposition were more uniform and had fewer domains than those deposited by the other methods. The ratio of picric acid-accessible amino groups to SPDP cross-linked rhodamine-accessible groups varied with the preparation method, suggesting reactant size-dependent difference in amine accessibility. We found a larger number of accessible amino groups on films prepared by vapor-phase deposition than on those prepared from solution deposition. The dilute vapor-phase deposition technique produced relatively few domains, and it should be a good choice for bioconjugation applications. There were appreciable differences in the films produced by each method. We suggest that these differences originate from differences in film rearrangement during annealing.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Morphology and Amine Accessibility of (3‐Aminopropyl) Triethoxysilane Films on Glass Surfaces

Wang

Vaughn

2008

Scanning

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“…For this purpose, alkylsilanes are grafted on glass (Ulman, 1991), silicon (Vandenberg et al, 1991;Kallury et al, 1994;Vrancken et al, 1995;Horr & Arora, 1997;Ek et al, 2003;Zhang & Srinivasan, 2004) or mica (Bezanilla et al, 1995;Lyubchenko et al, 1996;Tätte et al, 2003;Diez-Perez, 2004;Crampton et al, 2005Crampton et al, , 2006 surfaces. Generally, it is assumed that molecules adsorb in an ordered film on the surface.…”

Section: Imaging 2d Polymerized Filmmentioning

confidence: 99%

“…The mica lattice contains negative binding sites associated with Al and filled with potassium ions, K + . In fluid, these cations (K + ) can be removed or displaced, as shown by surface force apparatus and XPS, presenting a negative surface potential (Crampton et al, 2005). For instance, protonated AAPS molecules (due to pH) can replace potassium ions, so they can adsorb preferentially on the surface.…”

Section: Deposition In Aqueous Phasementioning

confidence: 99%

Tapping Mode AFM Imaging for Functionalized Surfaces

Candoni¹

2012

Atomic Force Microscopy Investigations Into Biology - From Cell to Protein

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“…[26][27][28][29][30] After APTES modification, the devices were further modified with poly-T ssDNA (1µM in Tris-HCl, pH 8). This process is described in the Supporting Information.…”

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confidence: 99%

Control and Detection of Organosilane Polarization on Nanowire Field-Effect Transistors

et al. 2007

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We demonstrated control and detection of UV-induced 3-aminopropyltriethoxysilane (APTES) polarization using silicon nanowire field-effect transistors made by top-down lithograph technology. The electric dipole moment in APTES films induced by UV-illumination was shown to produce negative effective charges. When individual dipoles were aligned with an externally applied electric field, the collective polarization can prevail over the UV-induced charges in the wires and give rise to an abnormal resistance enhancement in n-type wires. Real-time detection of hybridization of 15-mer poly-T/poly-A DNA molecules was performed, and the amount of hybridization-induced charges in the silicon wire was estimated. Based on these results, detection sensitivity of the wire sensors was discussed.Hetero-interfaces between organic and semiconductor oxides have attracted extensive attentions 1-4 due to the critical role of molecule assembly in the sensing electronics involving hybrid structures. APTES 5 (3-aminopropyltriethoxysilane) and other compounds such as PTS 6 (n-propyltrichlorosilane), OTS 7 (n-octadecyltrichlorosilane), TCTS 8 (n-triacontyltrichlorosilane), OTMS 9 (n-octadecyltrimethoxysilane), and AHT-MS 9 (n-aminoheptadecyltrimethoxysilane) with head-andtail functional groups are widely used interfacing molecules, and assembly of these molecules is essential in surfacemodification technologies. Silanization of oxidized semiconductor surfaces is a commonly employed scheme for functionalization of sensors. The functional groups would then provide binding sites for attachment of probe molecules, such as single-strand DNA (ssDNA), on the semiconductor sensing devices. The nanowire-based sensors have been demonstrated 10-14 as an ultra sensitive detector for probing molecular charges at the wire surface. However, surface modification of the functional groups on the nanowire surface is not a trivial task. Extensive studies in the surfacemodification were reported in the past years, 5-9 but issues concerning monolayer molecule ordering in terms of the electric dipole moment remain unexplored. Taking APTES as an example, in this study, we proposed a simple method to align the molecule dipoles, and the degree of alignment was examined by underneath Si-nanowire (SiNW) field effect transistors. This method provides a sensitive way for structure investigation of few molecules at the nanometer scale, which is otherwise unfeasible by the present-day examination tools. † These authors contributed equally to this work.

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Formation of Aminosilane-Functionalized Mica for Atomic Force Microscopy Imaging of DNA

Cited by 82 publications

References 34 publications

Morphology and Amine Accessibility of (3‐Aminopropyl) Triethoxysilane Films on Glass Surfaces

Morphology and Amine Accessibility of (3‐Aminopropyl) Triethoxysilane Films on Glass Surfaces

Tapping Mode AFM Imaging for Functionalized Surfaces

Control and Detection of Organosilane Polarization on Nanowire Field-Effect Transistors

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