Formation of Agarose-supported Liposomes by Polymer-assisted Method toward Biosensor

Peng, Zugui; Wada, Kentaro; Shimba, Kenta; Miyamoto, Yoshitaka; Yagi, T.

doi:10.1541/ieejeiss.141.646

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The stabilizing strategy of liposomes is broadly classified into three categories using nontoxic materials. (1) The gelation of the inner solution or the construction of the undercoats such as the cytoskeleton-like network has been attempted to prepare a stable liposome. − The gel network inside the liposome forms by temperature regulation , or light irrigation, mechanically reinforcing the liposome structure. (2) Incorporation of cholesterol into the membrane is another strategy to mechanically stabilize the liposome.…”

Section: Introductionmentioning

confidence: 99%

Long-Term Stable Liposome Modified by PEG-Lipid in Natural Seawater

Izumi,

Ji,

Koiwai

et al. 2024

ACS Omega

View full text Add to dashboard Cite

This paper describes the stabilization of liposomes using a PEGylated lipid, N-(methylpolyoxyethylene oxycarbonyl)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine sodium salt (DSPE-PEGs), and the evaluation of the survival rate in natural seawater for future environmental applications. Liposomes in natural seawater were first monitored by confocal microscopy, and the stability was compared among different lengths and the introduction ratio of DSPE-PEGs. The survival rate increased with an increase in the PEG ratio. In addition, the survival rate in different cationic solutions (Na + , K + , Mg 2+ , and Ca 2+ solutions) was studied to estimate the effects of the DSPE-PEG introduction. We propose that these variations in liposome stability are due to the cations, specifically the interaction between the poly(ethylene glycol) (PEG) chains and divalent ions, which contribute to making it difficult for cations to access the lipid membrane. Our studies provide insights into the use of PEG lipids and may offer a promising approach to the fabrication of liposomal molecular robots using different natural environments.

show abstract