Formation of a thiobarbituric‐acid‐reactive substance from deoxyribose in the presence of iron salts

Halliwell, Barry; Gutteridge, John M.C.

doi:10.1016/0014-5793(81)80114-7

Cited by 788 publications

(377 citation statements)

References 26 publications

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“…Damage to lipids and deoxyribose by iron-EDTA and hydrogen peroxide was markedly inhibited by the addition of a range of hydroxyl radical scavengers, superoxide dismutase and catalase (table 2) but not by urea, albumin and heat-denatured catalase which were included as controls for non-specific effects. Heat-denatured superoxide dismutase, however, retained some activity in the deoxyribose and phospholipid systems as previously observed [8].…”

Section: Resultssupporting

confidence: 82%

“…Heat-denatured superoxide dismutase did not totally lose its enzymic activity, as observed in [8], and this probably reflects recombination of released copper and protein to restore some dismutase activity.…”

Section: Discussionmentioning

confidence: 69%

“…The iron-EDTA complex can, however, readily decompose hydrogen peroxide in a Fenton-type sequence releasing OH' radicals which initiate lipid peroxidation. When a ferrous salt is mixed with EDTA the complex rapidly auto-oxidises to form the ferric complex [8]. However, addition of hydrogen peroxide to a ferric-EDTA complex still resulted in the formation of hydroxyl radicals, as shown by the inhibitory properties of the hydroxyl radical scavengers tested.…”

Section: Discussionmentioning

confidence: 99%

“…Fe"-EDTA + Hz02+ Fe3'-EDTA + OH' + OH- (8) Catalyse can interfere with lipid oxidation studies by non-specifically decomposing lipid peroxides or stimulating TBA reactivity [ 111. However, in this study it strongly inhibited lipid peroxidation and deoxyribose degradation, although its specificity was not required to substantiate involvement of hydrogen peroxide.…”

Section: Discussionmentioning

confidence: 99%

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Lipid peroxidation initiated by superoxide‐dependent hydroxyl radicals using complexed iron and hydrogen peroxide

Gutteridge

1984

FEBS Letters

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127

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Iron salts stimulate lipid peroxidation by decomposing lipid peroxides to produce alkoxyl and peroxyl radicals which initiate further oxidation. In aqueous solution ferrous salts produce OH' radicals, a reactive species able to abstract hydrogen atoms from unsaturated fatty acids, and so can initiate lipid peroxidation. When iron salts are added to lipids, containing variable amounts of lipid peroxide, the former reaction is favoured and OH' radicals contribute little to the observed rate of peroxidation. When iron is complexed with EDTA, however, lipid peroxide decomposition is prevented, but the complex reacts with hydrogen peroxide to form OH' radicals which are seen to initiate lipid peroxidation. Superoxide radicals appear to play an important part in reducing the iron complex.

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Section: Resultssupporting

confidence: 82%

Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Lipid peroxidation initiated by superoxide‐dependent hydroxyl radicals using complexed iron and hydrogen peroxide

Gutteridge

1984

FEBS Letters

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127

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“…16 In their method the malonaldehyde arises as a product of deoxyribose degradation by the HO radical. The product in combination with thiobarbituric acid forms a colored (2:1) thiobarbituric acid-malonaldehyde adduct that absorbs at 532 nm in acid solution.…”

Section: Methodsmentioning

confidence: 99%

Effect of psoralens on Fenton‐like reaction generating reactive oxygen species

et al. 2002

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Psoralens (psoralen, khellin, and visnagin) in 1 mM doses were shown to enhance the generation of reactive oxygen species, such as the hydroxyl radical (HO ⅐ ), the superoxide anion radical (O 2 Ϫ • ), and singlet oxygen ( 1 O 2 ), from the system generating chemiluminescence (CL), as well as free radicals in the absence of light. The system that generated CL was made up of CoCl 2 and H 2 O 2 . Incubation of psoralens in 0.2 mM doses with the generating system showed that only 8-methoxypsoralen and khellin have antioxidative effects. Antioxidative effects were also observed in the case of visnagin but in low concentration (0.05 mM). High doses of psoralens (1 mM) showed prooxidative effects. Measurements were done using a deoxyribose assay, the CL method, and spin-trapping with 5,5-dimethyl-1-pyrroline-N-oxide and 2,2,6,6-tetramethylpiperidine combined with electron spin resonance spectroscopy and spectrophotometry methods.

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A simple, rapid, highly sensitive and reproducible quantification method for plasma malondialdehyde by high‐performance liquid chromatography

Fukunaga

Yoshida

Nakazono

1998

Biomedical Chromatography

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Formation of a thiobarbituric‐acid‐reactive substance from deoxyribose in the presence of iron salts

Cited by 788 publications

References 26 publications

Lipid peroxidation initiated by superoxide‐dependent hydroxyl radicals using complexed iron and hydrogen peroxide

Lipid peroxidation initiated by superoxide‐dependent hydroxyl radicals using complexed iron and hydrogen peroxide

Effect of psoralens on Fenton‐like reaction generating reactive oxygen species

A simple, rapid, highly sensitive and reproducible quantification method for plasma malondialdehyde by high‐performance liquid chromatography

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