Formation of a stable DNA-protein bond by the action of a bifunctional alkylating mutagen (embichin-HN2) on chromatin

Characterization of DNA-protein crosslinks formed by treatment of L1210 cells and nuclei with bis(2-chloroethyl)methylamine (nitrogen mustard)

“…Trypsin treatment largely abolished this difference. Sokolov et al (1975) demonstrated that HN2 treatment of chromatin produced an increase in DNA-bound protein as assayed by gel filtration, ultracentrifugation, and hydroxylapatite chromatography. Recent investigations from this laboratory, using the technique of alkaline elution, have revealed proteinase-sensitive DNA cross-links in L1210 cells treated with HN2 (Ewig & Kohn, 1977).There is no direct evidence on the chemistry of these protein-DNA cross-links or on the kinds of proteins that are bound to the DNA.…”