Formation of a PSI–PSII megacomplex containing LHCSR and PsbS in the moss Physcomitrella patens

Furukawa, Ryo; Aso, Michiki; Fujita, Tomomichi; Akimoto, Seiji; Tanaka, Ryouichi; Tanaka, Ayumi; Yokono, Makio; Takabayashi, Akira

doi:10.1007/s10265-019-01138-2

Cited by 16 publications

(8 citation statements)

References 63 publications

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“…Considering that both CP26 and CP29 are involved in LHCII detachment from PSII and its migration towards PSI, it is possible that LHCSR‐dependent quenching of LHCII, when detached from PSII and/or connected to PSI, may be also related to LHCSR interaction with monomeric antennas. Recently, a megacomplex composed of PSII, PSI, PsbS, and LHCSR was reported in the case of Physcomitrella patens (Furukawa et al, ): The formation of such megacomplexes in C. reinhardtii and their possible role in PSI quenching needs to be investigated by additional work.…”

Section: Discussionmentioning

confidence: 99%

“…Considering that both CP26 and CP29 are involved in LHCII detachment from PSII and its migration towards PSI, it is possible that LHCSR-dependent quenching of LHCII, when detached from PSII and/or connected to PSI, may be also related to LHCSR interaction with monomeric antennas. Recently, a megacomplex composed of PSII, PSI, PsbS, and LHCSR was reported in the case of Physcomitrella patens(Furukawa et al, 2019): The formation of such megacomplexes in C. reinhardtii and their possible role in PSI quenching needs to be investigated by additional work.The specific role of CP26 and CP29 in state transitions and NPQ induction herein described reveals a strong difference in the function of monomeric antenna proteins among A. thaliana and C. reinhardtii.In plant, monomeric antenna are not essential for NPQ, and their absence does not abolish state transitions(Dall'Osto et al, 2017;Townsend et al, 2018), whereas in the latter, both adaptive mechanisms require the presence of CP26 and/or CP29.…”

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confidence: 99%

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Photosystem II antenna complexes CP26 and CP29 are essential for nonphotochemical quenching in Chlamydomonas reinhardtii

Cazzaniga

Kim

Bellamoli

et al. 2019

Plant Cell & Environment

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Photosystems must balance between light harvesting to fuel the photosynthetic process for CO2 fixation and mitigating the risk of photodamage due to absorption of light energy in excess. Eukaryotic photosynthetic organisms evolved an array of pigment‐binding proteins called light harvesting complexes constituting the external antenna system in the photosystems, where both light harvesting and activation of photoprotective mechanisms occur. In this work, the balancing role of CP29 and CP26 photosystem II antenna subunits was investigated in Chlamydomonas reinhardtii using CRISPR‐Cas9 technology to obtain single and double mutants depleted of monomeric antennas. Absence of CP26 and CP29 impaired both photosynthetic efficiency and photoprotection: Excitation energy transfer from external antenna to reaction centre was reduced, and state transitions were completely impaired. Moreover, differently from higher plants, photosystem II monomeric antenna proteins resulted to be essential for photoprotective thermal dissipation of excitation energy by nonphotochemical quenching.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Photosystem II antenna complexes CP26 and CP29 are essential for nonphotochemical quenching in Chlamydomonas reinhardtii

Cazzaniga

Kim

Bellamoli

et al. 2019

Plant Cell & Environment

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“…Pigments (chlorophyll a, b, violaxanthin, antheraxanthin, and zeaxanthin) were extracted from 20 mg of frozen leaf materials by homogenization in pre-cooled acetone at –30˚C, as described by Furukawa et al. (2019) .…”

Section: Methodsmentioning

confidence: 99%

“…Pigments (chlorophyll a, b, violaxanthin, antheraxanthin, and zeaxanthin) were extracted from 20 mg of frozen leaf materials by homogenization in pre-cooled acetone at -30˚C, as described by Furukawa et al (2019). Extracts were centrifuged for 5 min at 21,600 × g at 4°C, and the supernatant was analyzed by high performance liquid chromatography (HPLC) using a C18 column (YMC-Pack ODS-AL 250 mm in length, 4.6 mm in i.d.…”

Section: Analyses Of Leaf Pigmentsmentioning

confidence: 99%

Exposure to strong irradiance exacerbates photoinhibition and suppresses N resorption during leaf senescence in shade-grown seedlings of fullmoon maple (Acer japonicum)

Kitao

Yazaki²,

Tobita³

et al. 2022

Front. Plant Sci.

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Leaves of fullmoon maple (Acer japonicum) turn brilliant red with anthocyanins synthesis in autumn. Based on field observations, autumn coloring mainly occurs in outer-canopy leaves exposed to sun, whereas inner-canopy leaves remain green for a certain longer period before finally turn yellowish red with a smaller amount of anthocyanins. Here, we hypothesized that outer-canopy leaves protect themselves against photooxidative stress via anthocyanins while simultaneously shading inner canopy leaves and protecting them from strong light (holocanopy hypothesis). To test this hypothesis, we investigated photoinhibition and leaf N content during autumn senescence in leaves of pot-grown seedlings of fullmoon maple either raised under shade (L0, ≈13% relative irradiance to open) or transferred to full sunlight conditions on 5th (LH1), 12th (LH2), or 18th (LH3) Oct, 2021. Dry mass-based leaf N (Nmass) in green leaves in shade-grown seedlings was ≈ 30 mg N g-1 in summer. Nmass in shed leaves (25th Oct to 1st Nov) was 11.1, 12.0, 14.6, and 10.1 mg N g-1 in L0, LH1, LH2, and LH3 conditions, respectively. Higher Nmass was observed in shed leaves in LH2, compared to other experimental conditions, suggesting an incomplete N resorption in LH2. Fv/Fm after an overnight dark-adaptation, measured on 19th Oct when leaf N was actively resorbed, ranked L0: 0.72 > LH3: 0.56 > LH1: 0.45 > LH2: 0.25. As decreased Fv/Fm indicates photoinhibition, leaves in LH2 condition suffered the most severe photoinhibition. Leaf soluble sugar content decreased, but protein carbonylation increased with decreasing Fv/Fm across shade-grown seedlings (L0, LH1, LH2, and LH3) on 19th Oct, suggesting impaired photosynthetic carbon gain and possible membrane peroxidation induced by photooxidative stress, especially in LH2 condition with less N resorption efficiency. Although the impairment of N resorption seems to depend on the timing and intensity of strong light exposure, air temperature, and consequently the degree of photoinhibition, the photoprotective role of anthocyanins in outer-canopy leaves of fullmoon maple might also contribute to allow a safe N resorption in inner-canopy leaves by prolonged shading.

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“…In mosses, LHCSR and PsbS can migrate between PSII and PSI in order to quench excitation energy (Furukawa et al . 2019). Recent studies ( e.g .…”

Section: Introductionmentioning

confidence: 99%

Resistance of Antarctic moss Sanionia uncinata to photoinhibition: chlorophyll fluorescence analysis of samples from the western and eastern coasts of the Antarctic Peninsula

et al. 2021

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Interspecific differences in sensitivity of the Antarctic moss Sanionia uncinata from King George Island (KGI) and James Ross Island (JRI) to photoinhibitory treatment were studied in laboratory conditions using chlorophyll fluorescence techniques. Slow (Kautsky) and fast (OJIP) kinetics were used for the measurements. Samples were exposed to a short‐term (60 min) photoinhibitory treatment (PIT, 2000 μmol·m−2·s−1 PAR). The photoinhibitory treatment (PIT) led to photoinhibition which was indicated by the decrease in FV/FM and ΦPSII in KGI but not in JRI samples. However, this decrease was small and full recovery was reached 90 min after PIT termination. Non‐photochemical quenching (NPQ) was activated during the PIT, and rapidly relaxed during recovery. Early stages of photoinhibition showed a drop in FV/FM and ΦPSII to minimum values within the first 10 s of the PIT, with their subsequent increase apparent within fast (0–5 min PIT) and slow (5–50 min PIT) phases of adjustment. The PIT caused a decrease in the performance index (Pi_Abs), photosynthetic electron transport per reaction centre (RC) (ET0/RC). The PIT induced an increase in thermal dissipation per RC (DI0/RC), effectivity of thermal dissipation (Phi_D0), absorption per RC (ABS/RC) and trapping rate per RC (TR0/RC). In conclusion, PIT led to only slight photoinhibition followed by fast recovery in S. uncinata from KGI and JRI, since FV/FM and ΦPSII returned to pre‐photoinhibitory conditions. Therefore, S. uncinata might be considered resistant to photoinhibition even in the wet state. The KGI samples showed higher resistance to photoinhibition than the JRI samples.

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Formation of a PSI–PSII megacomplex containing LHCSR and PsbS in the moss Physcomitrella patens

Cited by 16 publications

References 63 publications

Photosystem II antenna complexes CP26 and CP29 are essential for nonphotochemical quenching in Chlamydomonas reinhardtii

Photosystem II antenna complexes CP26 and CP29 are essential for nonphotochemical quenching in Chlamydomonas reinhardtii

Exposure to strong irradiance exacerbates photoinhibition and suppresses N resorption during leaf senescence in shade-grown seedlings of fullmoon maple (Acer japonicum)

Resistance of Antarctic moss Sanionia uncinata to photoinhibition: chlorophyll fluorescence analysis of samples from the western and eastern coasts of the Antarctic Peninsula

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