Formation of a protection film on the human skin by microparticles

Abstract

Laser scanning microscopy and tape stripping, in combination with optical methods, were used to analyze the distribution and penetration of a barrier cream into the horny layer (stratum corneum) of the human skin under in vivo conditions. The barrier cream contained microparticles of 10 -100 µm loaded with antioxidant substances. The cream was designed for protection of the skin surface against the destructive action of free radicals, produced by systemically applied chemotherapeutic agents reaching the skin surface via the sweat. Both methods were able to demonstrate that the barrier cream was distributed homogeneously on the skin surface forming a protection film. A penetration into deeper parts of the stratum corneum (SC) was not observed. Absorption spectra of the unsolved tape (dotted line) and of the solved tape in ethanol (continuous line) of the first and second tape strips

Efficient Prevention Strategy against the Development of a Palmar-Plantar Erythrodysesthesia during Chemotherapy

“…This formulation has a double effect. First of all, the reservoir of the stratum corneum will be saturated by the ointment which reduces the penetration of the chemotherapeutic into the skin [19]. The second aspect is that the ointment contains microparticles with a high concentration of antioxidants, which form a protective layer on the skin surface.…”

“…The ointment has a high radical protection factor of 4,500 [17]. A detailed description of the formulation is given in Lademann et al [18,19]. The patients were requested to follow specific behavioral instructions by applying the ointment onto the palmar-plantar region at least twice daily starting 2 days before commencement of the chemotherapy.…”

Methods for the Evaluation of the Protective Efficacy of Sunscreen Products

“…A homogeneous spreading of the sunscreen from the application area was also observed into the neighboring nontreated skin areas. Homogeneous spreading represents a prerequisite for a good quality efficacy of a sunscreen product, which is determined by the homogeneity of the distribution of the sunscreen on the skin, as well as by the absorption properties of the UV filter substances and by the annihilation of the absorbed photon energy in the skin [36,37]. With a well-balanced filter combination entailing synergetic effects and the homogeneous distribution of these filters on the skin, a high protective efficacy can be achieved for the investigated sunscreen Anthelios XL Fluide Extreme (SPF 50+), using a small amount of filter substances.…”

Quantification of the Inhomogeneous Distribution of Topically Applied Substances by Optical Spectroscopy: Definition of a Factor of Inhomogeneity

“…The sunscreen contained 0.1% of the fluorescent dye fluorescein to clear-ly show the distribution of the sunscreen on the skin. The in vivo measurements were carried out using the laser scanning microscope Stratum (OptiScan, Melbourne, Australia) [21] . The system consists of a base station containing an argon laser ( = 488 nm), a spectrometer and a control unit.…”