Formation of a Bacteriostatic Surface on ZrNb Alloy via Anodization in a Solution Containing Cu Nanoparticles

Korniienko, Viktoriia; Oleshko, Oleksandr; Husak, Yevheniia; Deineka, Volodymyr; Holubnycha, Viktoriia Mykolaivna; Mishchenko, Oleg; Kazek-Kęsik, Alicja; Jakóbik‐Kolon, Agata; Pshenychnyi, Roman; Leśniak-Ziółkowska, Katarzyna; Kalinkevich, Oksana; Калинкевич, А. Н.; Pisarek, Marcin; Simka, Wojciech; Pogorielov, Мaksym

doi:10.3390/ma13183913

Cited by 14 publications

(13 citation statements)

References 39 publications

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“…As the electrolyte, a suspension of Ag nanoparticles (180 mg/L) in a solution containing organic chelating agent (NTA), Ca(OH) 2 , and KH 2 PO 4 was used [33]. Details of the PEO process were described in [34]. All samples were rinsed with distilled water and ultrasonically cleaned in deionized water and 2-propanol for 5 min prior to PEO treatment.…”

Section: Plasma Electrolytic Oxidationmentioning

confidence: 99%

“…On the next day, the medium was removed and U2OS cells were seeded on each sample and in the wells without samples (as positive control) at a cell density of 10 4 cells per well. Cell adhesion at 24 h and cell proliferation on samples were assessed by a Alamar blue colorimetric assay as previously described [34]. As a negative control, Alamar blue solution was added to the wells containing only culture medium without cells.…”

Section: Cell Culture Biocompatibility Testmentioning

confidence: 99%

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In Vitro Biological Characterization of Silver-Doped Anodic Oxide Coating on Titanium

et al. 2020

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Despite the high biocompatibility and clinical effectiveness of Ti-based implants, surface functionalization (with complex osteointegrative/antibacterial strategies) is still required. To enhance the dental implant surface and to provide additional osteoinductive and antibacterial properties, plasma electrolytic oxidation of a pure Ti was performed using a nitrilotriacetic acid (NTA)-based Ag nanoparticles (AgNP)-loaded calcium–phosphate solution. Chemical and structural properties of the surface-modified titanium were assessed using scanning electron microscopy (SEM) with energy dispersive X-ray (EDX) and contact angle measurement. A bacterial adhesion test and cell culture biocompatibility with collagen production were performed to evaluate biological effectiveness of the Ti after the plasma electrolytic process. The NTA-based calcium–phosphate solution with Ag nanoparticles (AgNPs) can provide formation of a thick, porous plasma electrolytic oxidation (PEO) layer enriched in silver oxide. Voltage elevation leads to increased porosity and a hydrophilic nature of the newly formed ceramic coating. The silver-enriched PEO layer exhibits an effective antibacterial effect with high biocompatibility and increased collagen production that could be an effective complex strategy for dental and orthopedic implant development.

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Section: Plasma Electrolytic Oxidationmentioning

confidence: 99%

Section: Cell Culture Biocompatibility Testmentioning

confidence: 99%

In Vitro Biological Characterization of Silver-Doped Anodic Oxide Coating on Titanium

et al. 2020

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“…Scanning electron microscopy (SEM), energy-dispersive X-ray (EDX), X-ray photoelectron spectroscopy, and contact angle (CA) methods were used for structural and surface characterization of the modified surface. Details of these experiments were detailed described in our previous paper [26].…”

Section: Surface Analysismentioning

confidence: 99%

“…It is possible to incorporate insoluble compounds/particles directly into the coating from electrolyte [25]. In our previous papers we proved fabrication of bioactive and antibacterial oxide coatings on Ti or ZrNb alloy with incorporation of nanoparticles [26][27][28]. PEO process provides formation of stable coating resistant to corrosion with slow ion release [26].…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility and Antibacterial Properties of ZnO-Incorporated Anodic Oxide Coatings on TiZrNb Alloy

et al. 2020

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In a present paper, we demonstrate novel approach to form ceramic coatings with incorporated ZnO nanoparticles (NPs) on low modulus TiZrNb alloy with enhanced biocompatibility and antibacterial parameters. Plasma Electrolytic Oxidation (PEO) was used to integrate ZnO nanoparticles (average size 12–27 nm), mixed with Ca(H2PO2)2 aqueous solution into low modulus TiZrNb alloy surface. The TiZrNb alloys with integrated ZnO NPs successfully showed higher surface porosity and contact angle. XPS investigations showed presence of Ca ions and absence of phosphate ions in the PEO modified layer, what explains higher values of contact angle. Cell culture experiment (U2OS type) confirmed that the surface of as formed oxide-ZnO NPs demonstrated hydrophobic properties, what can affect primary cell attachment. Further investigations showed that Ca ions in the PEO coating stimulated proliferative activity of attached cells, resulting in competitive adhesion between cells and bacteria in clinical situation. Thus, high contact angle and integrated ZnO NPs prevent bacterial adhesion and considerably enhance the antibacterial property of TiZrNb alloys. A new anodic oxide coating with ZnO NPs could be successfully used for modification of low modulus alloys to decrease post-implantation complications.

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“…The PEO surfaces were made suitable for successful bone integration [6] and to support proliferation of mesenchymal stem cells [7]. On the other hand, the PEO layers were rendered bacteriostatic properties via incorporation of Ag and Cu nanoparticles [8,9,10]. The surface morphology of the PEO layers can also be modified in order to achieve the desired structure which could improve cellular adhesion and viability [11].…”

Section: Introductionmentioning

confidence: 99%

A New Solution for an Electrochemical Modification of Titanium Surface

Kyrylenko¹,

Oleshko²,

Warchoł³

et al. 2020

J. Nano- Electron. Phys.

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Dental/osseous implants manufactured of titanium (Ti) have become a routine and affordable method in medical practice. To increase the overall safety and longtime stability of the implants further, the sophisticated approaches have been investigated in order to supply the implants with the bioactive surface layers. They have to serve two purposes: to increase the osseointegration capacity of the implants and to reduce the chances of bacterial growth and formation of bacterial biofilms leading to periimplantitis. Plasma electrolytic oxidation (PEO) is becoming a promising method to introduce functionalized surface layers on a metal substrate. Various PEO protocols have been suggested in order to achieve better biocompatibility of the dental implants and to increase their resistance to bacterial infections. It was also suggested that the PEO layers could increase resistance to corrosion on the surfaces of metallic implants. The dynamic processes running on the surface of Ti during the PEO processing still require efforts to fully understand the molecular mechanisms of the formation of hard and porous oxide surface layers. We and others have already shown that addition of a chelating agent to the bath electrolyte leads to better outcomes in the morphology and functional characteristics of the implants. Here we report in depth characterization of the PEO parameters and the produced PEO surface layers using the bath electrolyte containing another widely used chelating agent, nitrilotriacetic acid (NTA) along with potassium phosphate and calcium formate. The results will contribute to further understanding the mechanisms of the PEO process and to establishing routine protocols for commercial exploitation of the PEO method.

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Formation of a Bacteriostatic Surface on ZrNb Alloy via Anodization in a Solution Containing Cu Nanoparticles

Cited by 14 publications

References 39 publications

In Vitro Biological Characterization of Silver-Doped Anodic Oxide Coating on Titanium

In Vitro Biological Characterization of Silver-Doped Anodic Oxide Coating on Titanium

Biocompatibility and Antibacterial Properties of ZnO-Incorporated Anodic Oxide Coatings on TiZrNb Alloy

A New Solution for an Electrochemical Modification of Titanium Surface

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