Formation of 2-ketogluconate from glucose by a cell-free preparation of Pseudomonas aeruginosa

Claridge, C. A.; Werkman, C. H.

doi:10.1016/0003-9861(53)90440-8

Cited by 19 publications

(8 citation statements)

References 17 publications

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“…Similarities are evident in the enzyme system from M. pseudomallei described in this report and those derived from Pseudomonas fluorescens (Wood and Schwerdt, 1953) and Pseudnomnas aeruginosa (Claridge and Werkman, 1953). In each case, activity was contained wholly within the fractions precipitated by (NH4)2S04 and added cofactor requirements were not demonstrated.…”

Section: Discussionsupporting

confidence: 76%

HEXOSE OXIDATION BY AN ENZYME SYSTEM OF MALLEOMYCES PSEUDOMALLEI

Dowling

Levine

1956

J Bacteriol

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Section: Discussionsupporting

confidence: 76%

HEXOSE OXIDATION BY AN ENZYME SYSTEM OF MALLEOMYCES PSEUDOMALLEI

Dowling

Levine

1956

J Bacteriol

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“…The complexity of glucose metabolism in Pseudomonas aeruginosa was revealed by the work of Stokes &Campbell (1951), Claridge &Werkman(1953,1954 and Wang et al (1959). Glucose may be oxidized directly to gluconate and 2-oxogluconate before phosphorylation, or may be phosphorylated directly and then oxidized.…”

mentioning

confidence: 99%

The regulation of transport of glucose, gluconate and 2-oxogluconate and of glucose catabolism in Pseudomonas aeruginosa

Whiting

Midgley

Dawes

1976

Biochemical Journal

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1. The induction by glucose and gluconate of the transport systems and catabolic enzymes for glucose, gluconate and 2-oxogluconate was studied with Pseudomonas aeruginosa PAO1 growing in a chemostat under conditions of nitrogen limitation with citrate as the major carbon source. 2. In the presence of a residual concentration of 30mM-citrate an inflowing glucose concentration of 6-8 mM was required to induce the glucose-transport system and associated catabolic enzymes. When the glucose concentration was raised to 20mM the glucose-transport system was repressed, but the transport system for gluconate, and at higher glucose concentrations, that for 2-oxogluconate, were induced. No repression of the glucose-catabolizing enzymes occurred at the higher inflowing glucose concentrations. 3. In the presence of 30mM-citrate no marked threshold concentration was required for the induction of the gluconate-transport system by added gluconate. 4. In the presence of 30mM-citrate and various concentrations of added glucose and gluconate, the activity of the glucose-transport system accorded with the proposal that a major factor concerned in the repression of this system was the concentration of gluconate, produced extracellularly by glucose dehydrogenase. 5. This proposal was supported by chemostat experiments with mutants defective in glucose dehydrogenase. Such mutants showed no repression of the glucose-transport system by high inflowing concentrations, but with a mutant apparently defective only in glucose dehydrogenase, the addition of gluconate caused repression of the glucose-transport system. 6. Studies with the mutants showed that both glucose and gluconate can induce the enzymes of the Entner-Doudoroff system, whereas for the induction of the gluconate-transport system glucose must be converted into gluconate.

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“…I), involving both direct oxidative and phosphorylative routes of metabolism (Stokes & Campbell, I951 ;Claridge & Werkman, 1953, 1954Wang, Stern & Gilmour, 1959;Roberts, Midgley & Dawes, 1973). This apparent complexity of metabolism has been clarified by the discovery that these two routes occur in different compartments of the cell.…”

Section: Introductionmentioning

confidence: 99%

The Role of Glucose Limitation in the Regulation of the Transport of Glucose, Gluconate and 2-Oxogluconate, and of Glucose Metabolism in Pseudomonas aeruginosa

Whiting¹,

Midgley²,

Dawes³

1976

Journal of General Microbiology

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The pathway of glucose metabolism in Pseudomonas aeruginosa was regulated by the availability of glucose and related compounds. On changing from an ammonium limitation to a glucose limitation, the organism responded by adjusting its metabolism substantially from the extracellular direct oxidative pathway to the intracellular phosphorylative route. This change was achieved by repression of the transport systems for gluconate and a-oxogluconate and of the associated enzymes for 2-oxogluconate metabolism and gluconate kinase, while increasing the levels of glucose transport, hexokinase and glucose 6-phosphate dehydrogenase. The role of gluconate, produced by the action of glucose dehydrogenase, as a major inhibitory factor for glucose transport, and the possible significance of these regulatory mechanisms to the organism in its natural environment, are discussed.

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Formation of 2-ketogluconate from glucose by a cell-free preparation of Pseudomonas aeruginosa

Cited by 19 publications

References 17 publications

HEXOSE OXIDATION BY AN ENZYME SYSTEM OF MALLEOMYCES PSEUDOMALLEI

HEXOSE OXIDATION BY AN ENZYME SYSTEM OF MALLEOMYCES PSEUDOMALLEI

The regulation of transport of glucose, gluconate and 2-oxogluconate and of glucose catabolism in Pseudomonas aeruginosa

The Role of Glucose Limitation in the Regulation of the Transport of Glucose, Gluconate and 2-Oxogluconate, and of Glucose Metabolism in Pseudomonas aeruginosa

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