Formation mechanisms of chitosan-silica hybrid materials and its performance as solid support for KR-12 peptide adsorption: Impact on KR-12 antimicrobial activity and proteolytic stability

Diosa, Johnatan; Guzmán, Fanny; Bernal, Claudia; Mesa, Mónica

doi:10.1016/j.jmrt.2019.11.029

Cited by 25 publications

(18 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The colloidal behavior of the CS8 sample can be explained by the full-charge charge screening after protein adsorption [ 19 ], as corroborated by the mean ζ -value around zero ( Figure 2(d) ). Most likely, the protein-surface affinity could be higher in CS8 than CS7, CS6, and S materials because of the higher chitosan percentage and hydrophilic character, reported by Diosa et al [ 10 , 18 ].…”

Section: Resultsmentioning

confidence: 99%

“…It is also a provisional extracellular matrix (ECM) cell support for tissue repair through extracellular binding sites [6]. Functional grafts for wound healing can be obtained by modifying hydrogels with cells [7][8][9], biomolecules such as antioxidants, antimicrobial acids, and peptides [10][11][12] among others.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, silica-fibrin hydrogel preserves the scaffold capacity for cell proliferation [ 23 ]. Some authors have shown synergic effects of hybrid chitosan-silica materials on wound healing [ 24 , 25 ] and improved biofunctionality [ 10 ]. Chitosan also improved the hemostatic behavior of mesoporous silica, enhancing the behavior of the fibrin network as a physical barrier to prevent a hemorrhage, in in vivo and in vitro experiments [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Improving Fibrin Hydrogels’ Mechanical Properties, through Addition of Silica or Chitosan-Silica Materials, for Potential Application as Wound Dressings

Becerra

Múnera

Galeano

et al. 2021

International Journal of Biomaterials

Self Cite

View full text Add to dashboard Cite

Fibrin is a protein-based hydrogel formed during blood coagulation. It can also be produced in vitro from human blood plasma, and it is capable of resisting high deformations. However, after each deformation process, it loses high amounts of water, which subsequently makes it mechanically unstable and, finally, difficult to manipulate. The objective of this work was to overcome the in vitro fibrin mechanical instability. The strategy consists of adding silica or chitosan-silica materials and comparing how the different materials electrokinetic-surface properties affect the achieved improvement. The siliceous materials electrostatic and steric stabilization mechanisms, together with plasma protein adsorption on their surfaces, were corroborated by DLS and ζ-potential measurements before fibrin gelling. These properties avoid phase separation, favoring homogeneous incorporation of the solid into the forming fibrin network. Young’s modulus of modified fibrin hydrogels was evaluated by AFM to quantitatively measure stiffness. It increased 2.5 times with the addition of 4 mg/mL silica. A similar improvement was achieved with only 0.7 mg/mL chitosan-silica, which highlighted the contribution of hydrophilic chitosan chains to fibrinogen crosslinking. Moreover, these chains avoided the fibroblast growth inhibition onto modified fibrin hydrogels 3D culture observed with silica. In conclusion, 0.7 mg/mL chitosan-silica improved the mechanical stability of fibrin hydrogels with low risks of cytotoxicity. This easy-to-manipulate modified fibrin hydrogel makes it suitable as a wound dressing biomaterial.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Improving Fibrin Hydrogels’ Mechanical Properties, through Addition of Silica or Chitosan-Silica Materials, for Potential Application as Wound Dressings

Becerra

Múnera

Galeano

et al. 2021

International Journal of Biomaterials

Self Cite

View full text Add to dashboard Cite

show abstract

“…Studies have been focused on the design of AMP carriers to solve this problem (Lewies et al, 2017;Nordström et al, 2019). The presence of chitosan-silica solid support of KR-12 peptide can protect it be hydrolyzed by α-trypsin, and the degree of protection is increased by 38% compared with the free KR-12 (Diosa et al, 2020). However, several enzymes, such as protease 65, esterase 66 and phosphatase 67, cut the blocking group of the peptide and trigger the self-assembly of the peptide, which positively affects AMPs (Tian et al, 2015).…”

Section: Proteasesmentioning

confidence: 99%

Antimicrobial Peptides: Classification, Design, Application and Research Progress in Multiple Fields

et al. 2020

View full text Add to dashboard Cite

Antimicrobial peptides (AMPs) are a class of small peptides that widely exist in nature and they are an important part of the innate immune system of different organisms. AMPs have a wide range of inhibitory effects against bacteria, fungi, parasites and viruses. The emergence of antibiotic-resistant microorganisms and the increasing of concerns about the use of antibiotics resulted in the development of AMPs, which have a good application prospect in medicine, food, animal husbandry, agriculture and aquaculture. This review introduces the progress of research on AMPs comprehensively and systematically, including their classification, mechanism of action, design methods, environmental factors affecting their activity, application status, prospects in various fields and problems to be solved. The research progress on antivirus peptides, especially anti-coronavirus (COVID-19) peptides, has been introduced given the COVID-19 pandemic worldwide in 2020.

show abstract

“…The other signicant band for CN was observed at 1411 cm À1 owing to -CH 2 wagging. 21 The XRD pattern of the prepared magnetic MCM-48 is presented in Fig. 1.…”

Section: Characterization Of Cmcm-48mentioning

confidence: 99%

Chitosan/MCM-48 nanocomposite as a potential adsorbent for removing phenol from aqueous solution

2020

View full text Add to dashboard Cite

show abstract

Formation mechanisms of chitosan-silica hybrid materials and its performance as solid support for KR-12 peptide adsorption: Impact on KR-12 antimicrobial activity and proteolytic stability

Cited by 25 publications

References 58 publications

Improving Fibrin Hydrogels’ Mechanical Properties, through Addition of Silica or Chitosan-Silica Materials, for Potential Application as Wound Dressings

Improving Fibrin Hydrogels’ Mechanical Properties, through Addition of Silica or Chitosan-Silica Materials, for Potential Application as Wound Dressings

Antimicrobial Peptides: Classification, Design, Application and Research Progress in Multiple Fields

Chitosan/MCM-48 nanocomposite as a potential adsorbent for removing phenol from aqueous solution

Contact Info

Product

Resources

About