Formation in Vitro of 5a-Dihydrotestosterone and other 5a-Reduced Metabolites of<sup>3</sup>H-Testosterone by the Seminiferous Tubules and Interstitial Tissue from Immature and Mature Rat Testes1

Folman, Y.; Ahmad, Nazir; Sowell, John; Eik‐Nes, Kristen B.

doi:10.1210/endo-92-1-41

Cited by 83 publications

(25 citation statements)

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“…Blood testosteronel in the female and prepubertal male is derived from secreted andros-4-ene-3,17-dione (androstenedione). Additionally, there is now evidence that testosterone, although the major circulating androgen, is converted peripherally by target tissue and perhaps other sites to 5a-androstan-17f-ol-3-one (dihydrotestosterone, DHT) which appears to be an important steroid messenger in sexual target tissue (7)(8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

3α-Androstanediol Kinetics in Man

Kinouchi¹,

Horton²

1974

J. Clin. Invest.

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A B S T R A C T Kinetics of 5a-androstane-3a, 17p-diol (3a-diol) were studied in man. Clearance than expected from information on in vitro binding in plasma. The kinetic data is of interest since 3a-diol has a calculated inner pool (Vi) volume of 12-14 liters, similar to 1713-hydroxyandrost-4-en-3-one (testosterone) and 5a-androstan-1713-ol-3-one (dihydrotestosterone), but the calculated outer pool (V2) of 33.5 liters is very large as are the metabolic rate and transfer constants. In contrast to testosterone and dihydrotestosterone, 3a-diol, although bound to sex hormone binding globulin, has a high metabolic clearance of which a large fraction represents extrahepatic (splanchnic) metabolism. A production rate of 3a-diol similar to dihydrotestosterone together with rather unique kinetic characteristics encourages further investigation of the biological role of this potent androgen.

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Section: Introductionmentioning

confidence: 99%

3α-Androstanediol Kinetics in Man

Kinouchi¹,

Horton²

1974

J. Clin. Invest.

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“…The incubated seminiferous tubules were remarkably free of interstitial tissue, but the latter tissue fraction [4] contained blood vessels, germinal cells and fragments of seminiferous tubules. By histological criteria the separated tissue appeared viable, although both tissue fractions showed signs of mechanical trauma to some cells [16]. When this type experiment in vitro was done with testes from rats 45 days old, the concentration of 3H-DHT was about the same at the end of a 2-hour incubation with either Leydig cells or seminiferous tubules [16].…”

Section: T and Dht In The Isolated Dog Testismentioning

confidence: 92%

“…By histological criteria the separated tissue appeared viable, although both tissue fractions showed signs of mechanical trauma to some cells [16]. When this type experiment in vitro was done with testes from rats 45 days old, the concentration of 3H-DHT was about the same at the end of a 2-hour incubation with either Leydig cells or seminiferous tubules [16].…”

Section: T and Dht In The Isolated Dog Testismentioning

confidence: 92%

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Production and Secretion of 5α-Dihydrotestosterone by Male Reproductive Organs

Eik‐Nes¹

1971

Gynecol Obstet Invest

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The androgen 5Α-dihydrotestosterone (DHT) has been isolated and identified in testicular tissue (rats, dogs) and in testicular venous blood of the canine testis. Conversion of ³H-testosterone (T) to ³H-DHT in vitro by testicular tissue from rats treated with follicle-stimulating hormone in vivo tended to increase. No such effect of human chorionic gonadotropin treatment could be observed. The Leydig cells of testes from mature rats contribute little to the formation in vitro of ³H-DHT from ³H-T. Both these androgens can be found in spermatic lymph and in epididymal tissue of the dog. In an isolated prostate preparation where the gland is infused via the prostatic arteries with the animal’s arterial blood at a constant rate, DHT can be found in effluent blood. The dog prostate both produces and secretes DHT. The purpose of DHT production and secretion by male reproductive organs is discussed.

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“…Many studies have been published concerning the in vitro conversion of testosterone and its precursors by isolated seminiferous tubules and interstitial tissue (Dufau et al,1971 ;Parvinen and Niemi,1971 ;Cooke et al, 1972 ;Rivarola and Podesta, 1972;Folman et a/., 1973).…”

Section: Introductionmentioning

confidence: 99%