Abstract:Natural polysaccharides are the most widely used biopolymers for green synthesis of eco-friendly silver nanoparticles (AgNPs). In a previous study, a high molecular weight (MW) fraction of exopolysaccharides (EPS) produced by a medicinal fungus Cs-HK1 has been shown useful for green and facile synthesis of AgNPs in water. This study was to further evaluate the effects of molecular properties of EPS on the formation, stability and properties of AgNPs with different EPS fractions at various pH conditions. Three … Show more
“…Gelatin could be functioned as stabilizing agents in the biosynthesis of AgNPs as positively charged gelatin can be electrostatically adsorbed on negatively charged AgNPs to form gelatin-coated AgNPs. Presence of protein in the reduction solution of O. limnetica acts as a capping agent and provides additional stability to AgNPs as well as gelatin exhibited well-capping activity 55 . Figure 2 FT-IR spectrum verified by making KBr disc with O. limnetica extract ( A ) and biosynthesized Oscillatoria - gelatin capped silver nanoparticles ( B ).…”
Over the last decade, an extensive range of consumer products containing manufactured silver nanoparticles (AgNPs) have been progressively used. The unfitting usage and discharge of these materials can enable passage of AgNPs into the aquatic ecosystem causing prospective toxicological consequence. The present study shed new lights on the phycotoxicity of small (8.47–17.66 nm) and stable Oscillatoria reduced gelatin-capped silver nanoparticles (OG-AgNPs) fabricated using a completely green synthetic technique. In this work, estimating of the possible toxic effects of OG-AgNPs on two freshwater microalgae Chlorella vulgaris and Chlorella minutissima was carried. This study found that, the growth of cells and photosynthetic pigment inhibitory effects of OG-AgNPs exhibit a significant increase with increasing time and concentration compared to control. Based on the IC50 value C. vulgaris (3.705 μg/mL) was found to be more sensitive to OG-AgNPs than C. minutissima (5.8 μg/mL). This study revealed that OG-AgNPs exhibit potent phycotoxic effect against Chlorella species. Finally, the negative effect of OG-AgNPs on aquatic algae and these modifications might have severe effects on structure and function of aquatic ecosystems. Besides, the biosynthesized OG-AgNPs showed a catalytic activity in the reduction of hydrogen peroxide, one of the reactive oxygen species that represent a major threat to biological systems. This method pretends an auspicious non-skill dependent technique with a good sensitivity for determination of H2O2 concentration, particularly at trace ppm level for applying in numerous domains such as medical and industrial processes.
“…Gelatin could be functioned as stabilizing agents in the biosynthesis of AgNPs as positively charged gelatin can be electrostatically adsorbed on negatively charged AgNPs to form gelatin-coated AgNPs. Presence of protein in the reduction solution of O. limnetica acts as a capping agent and provides additional stability to AgNPs as well as gelatin exhibited well-capping activity 55 . Figure 2 FT-IR spectrum verified by making KBr disc with O. limnetica extract ( A ) and biosynthesized Oscillatoria - gelatin capped silver nanoparticles ( B ).…”
Over the last decade, an extensive range of consumer products containing manufactured silver nanoparticles (AgNPs) have been progressively used. The unfitting usage and discharge of these materials can enable passage of AgNPs into the aquatic ecosystem causing prospective toxicological consequence. The present study shed new lights on the phycotoxicity of small (8.47–17.66 nm) and stable Oscillatoria reduced gelatin-capped silver nanoparticles (OG-AgNPs) fabricated using a completely green synthetic technique. In this work, estimating of the possible toxic effects of OG-AgNPs on two freshwater microalgae Chlorella vulgaris and Chlorella minutissima was carried. This study found that, the growth of cells and photosynthetic pigment inhibitory effects of OG-AgNPs exhibit a significant increase with increasing time and concentration compared to control. Based on the IC50 value C. vulgaris (3.705 μg/mL) was found to be more sensitive to OG-AgNPs than C. minutissima (5.8 μg/mL). This study revealed that OG-AgNPs exhibit potent phycotoxic effect against Chlorella species. Finally, the negative effect of OG-AgNPs on aquatic algae and these modifications might have severe effects on structure and function of aquatic ecosystems. Besides, the biosynthesized OG-AgNPs showed a catalytic activity in the reduction of hydrogen peroxide, one of the reactive oxygen species that represent a major threat to biological systems. This method pretends an auspicious non-skill dependent technique with a good sensitivity for determination of H2O2 concentration, particularly at trace ppm level for applying in numerous domains such as medical and industrial processes.
“…It belongs to a group of terpenoids renown for their anti-inflammatory, anti-proliferative, and pro-apoptotic effects 41 , 42 . Several studies have revealed that UA also possesses anti-cancer properties 26 , 43 – 46 , and recent studies demonstrated reduced proliferation and invasive potential of melanoma cell lines exposed to UA 47 – 49 . Although the effects of UA on cellular metabolism are still not well defined, several studies indicate that it damages mitochondria, as it induces depolarization and leads to ROS accumulation in different cancer cell types, such as breast cancer 50 , hepatocellular carcinoma and melanoma 26 .…”
The prognosis of metastatic melanoma remains poor due to de novo or acquired resistance to immune and targeted therapies. Previous studies have shown that melanoma cells have perturbed metabolism and that cellular metabolic pathways represent potential therapeutic targets. To support the discovery of new drug candidates for melanoma, we examined 180 metabolic modulators, including phytochemicals and anti-diabetic compounds, for their growth-inhibitory activities against melanoma cells, alone and in combination with the BRAF inhibitor vemurafenib. Two positive hits from this screen, 4-methylumbelliferone (4-MU) and ursolic acid (UA), were subjected to validation and further characterization. Metabolic analysis showed that 4-MU affected cellular metabolism through inhibition of glycolysis and enhanced the effect of vemurafenib to reduce the growth of melanoma cells. In contrast, UA reduced mitochondrial respiration, accompanied by an increase in the glycolytic rate. This metabolic switch potentiated the growth-inhibitory effect of the pyruvate dehydrogenase kinase inhibitor dichloroacetate. Both drug combinations led to increased production of reactive oxygen species, suggesting the involvement of oxidative stress in the cellular response. These results support the potential use of metabolic modulators for combination therapies in cancer and may encourage preclinical validation and clinical testing of such treatment strategies in patients with metastatic melanoma.
“…The phytochemicals (PTC) inherent in DGL, as observed by many authors is bloated with flavonoid, alkaloid, Tannin and Saponin, the major bioactive compounds in all edible plants [64,65]. Owing to the intrinsic PTC in DGL, it serves as a good reducing agent for the synthesis of the formulated DGL-AgNPs, a sequel to its affinity towards Ag ions formation [66] when compared to wet Gongronema latifolium as we have previously reported [13].…”
In-situ biosynthesis of silver nanoparticles for antibacterial activities doped with aqueous extract of dry Gongronema Latifolium (DGL) is presented in this work. The absorbance was determined using a UV-Visible analysis with a peak of 428 nm. The obtained particles confirmed by the TEM image analysis were spherical in shape with an average particle size between 5 and 20 nm. A single crystalline phase of FCC was confirmed by the powder. The functional groups showed the phytochemical inherent in the sample. The colloidal solution of DGL-AgNPs with MIC of 10 µg/ml showed a noteworthy functional susceptibility against the bacterial strains, particularly, K. pneumonia with susceptibility greater than gentamicin. Based on these observations, the formulated DGL-AgNPs show strong bactericidal against the pathogens with facile, benign, eco-friendly, biocompatibility and innocuous synthesis procedures. This can serve as an efficient bactericidal agent.
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