Formation and fluidity measurement of supported lipid bilayer on polyvinyl chloride membrane

Kobayashi, Takuji; Kono, Akiteru; Futagawa, Masato; Sawada, Kazuaki; Tero, Ryugo

doi:10.1063/1.4866633

Cited by 4 publications

(8 citation statements)

References 32 publications

(53 reference statements)

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“…Photobleaching was performed using 1778-fold stronger laser power than that for observation. 40) Furthermore, we determined whether the following two experimental conditions satisfied the hypothesis related to the diffusion law, which were explained in Ref. 43.…”

Section: Measurement Of Diffusion Coefficientmentioning

confidence: 99%

“…The lateral lipid mobility of the DOPC-SLBs before and after the DBD plasma irradiation was quantitatively evaluated by FRAP under the CLSM. 40) When part of the lipid bilayer containing a dye-labeled lipid (Rb-DOPE in this study) is irradiated by an intense excitation light, the fluorescent dye in the lipid bilayer is photobleached. If lipid molecules diffuse laterally in a bilayer, the fluorescence intensity of the photobleached region recovers because of the lateral First, we performed FRAP before plasma irradiation, and after plasma irradiation, we measured FRAP again in the same sample (Fig.…”

Section: Measurement Of Diffusion Coefficientmentioning

confidence: 99%

See 1 more Smart Citation

Reduction in lateral lipid mobility of lipid bilayer membrane by atmospheric pressure plasma irradiation

Suda

Tero

Yamashita

et al. 2016

Jpn. J. Appl. Phys.

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Plasma medicine is an emerging research field in which various applications of electrical discharge, especially in the form of nonequilibrium plasma at atmospheric pressure, are examined, for example, the application of plasma to biological targets for various purposes such as selective killing of tumor cells and blood stanching. We have focused on the behavior of an artificial cell membrane system at the solid-liquid interface. To evaluate the lateral lipid mobility, we measured the diffusion coefficient of the supported lipid bilayer (SLB) composed of dioleoylphosphatidylcholine with fluorescence recovery after photobleaching by confocal laser scanning microscopy. It was found that the diffusion coefficient was decreased by plasma irradiation and that the diffusion coefficient decreasing rate proceeded with increasing plasma power. We investigated the effects of stimulation with an equilibrium chemical, H 2 O 2 , on the SLB and confirmed that the diffusion coefficient did not change at least up to a H 2 O 2 concentration of 5 mM. These results indicate that transient active species generated by plasma play critical roles in the reduction in SLB fluidity. The effects of the two generated major oxidized lipid species, hydroxyl-or hydroperoxy-phosphatidylcholine (PC) and acyl-chain-truncated PCs terminated with aldehyde or carboxyl group, on lateral lipid mobility are discussed.

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Section: Measurement Of Diffusion Coefficientmentioning

confidence: 99%

Section: Measurement Of Diffusion Coefficientmentioning

confidence: 99%

Reduction in lateral lipid mobility of lipid bilayer membrane by atmospheric pressure plasma irradiation

Suda

Tero

Yamashita

et al. 2016

Jpn. J. Appl. Phys.

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“…In previous reports using FRAP with a much larger bleaching diameter, the diffusion coefficient of DOPC on a SiO 2 or quartz substrate was reported to be D = 2.03−3.2 μm 2 /s. 31,32 With fluorescence correlation spectroscopy, a diffusion coefficient D = 2.7 μm 2 /s was also reported for DOPC on a mica substrate. 33 The diffusion coefficient obtained with this method appears reasonable.…”

Section: Resultsmentioning

confidence: 86%

Evaluation of Lateral Diffusion of Lipids in Continuous Membranes between Freestanding and Supported Areas by Fluorescence Recovery after Photobleaching

2019

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The lateral diffusion of lipids is a key factor when functionalizing artificial planar bilayer lipid membranes (BLMs). Fluorescence recovery after photobleaching (FRAP) is an established method for evaluating the fluidity of BLMs by the quantitative determination of the diffusion coefficient. However, a BLM with a uniform diffusion coefficient is usually assumed for analysis. In addition, when the BLM to be evaluated is small, the spread of a bleached circle caused by lateral diffusion during the bleaching process and the divergence of the laser used for bleaching interfere with the quantitative analysis. In this study, a numerical calculation was adopted to make it possible to analyze the continuous BLM between freestanding and supported areas, where the diffusion coefficients change depending on the presence or absence of an interaction with the substrate. A quantitative evaluation independent of such bleaching conditions as the bleaching diameter was also ensured by incorporating the spreading effect of the bleached circle in the calculation, which was employed to analyze a freestanding BLM with a diameter of only a few micrometers. By comparing calculations and experiments on FRAP recovery curves, we found that there are multiple diffusion elements and high diffusion barriers at the boundary between the freestanding and supported areas in a BLM over a SiO 2 /Si microwell substrate.

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“…4 This list includes quartz crystal microbalance with dissipation monitoring (QCM-D), 5,6 localized surface plasmon resonance sensing (nano-plasmonic sensing), 7 atomic force microscopy, 8 infrared spectroscopy, 9 optical waveguide light mode spectroscopy, 10 and fluorescence imaging. 11,12 Individual monitoring techniques may necessitate specific measurement conditions or sensor composition. For example, to monitor bilayer properties with Fourier transform infrared spectroscopy, a bilayer must be formed on an internal reflection element commonly made of germanium, 13 zinc selenide, 14 or silicon.…”

Section: Introductionmentioning

confidence: 99%

“…The advantages of the vesicle fusion method of SLB formation include the ease with which it can be performed, and its compatibility with silicon dioxide (SiO 2 ) and silicon nitride (Si 3 N 4 ) substrates that are in turn compatible with a wide range of measurement techniques. 7,10,12,16,17 To perform vesicle fusion, vesicles are exposed to a substrate until a critical surface concentration is reached, whereupon vesicles fuse and rupture to form a uniform supported lipid bilayer. The rupture of vesicles is facilitated by the hydrophilic character of the substrate; therefore, vesicle fusion necessitates a surface with relatively high hydrophilicity as is commonly found in metal oxides.…”

Section: Introductionmentioning

confidence: 99%

Influence of sensor composition on nanoparticle and protein interaction with supported lipid bilayers

Reardon-Lochbaum,

Senanayake,

Amaro Marquez

et al. 2024

Environ. Sci.: Nano

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Formation and fluidity measurement of supported lipid bilayer on polyvinyl chloride membrane

Cited by 4 publications

References 32 publications

Reduction in lateral lipid mobility of lipid bilayer membrane by atmospheric pressure plasma irradiation

Reduction in lateral lipid mobility of lipid bilayer membrane by atmospheric pressure plasma irradiation

Evaluation of Lateral Diffusion of Lipids in Continuous Membranes between Freestanding and Supported Areas by Fluorescence Recovery after Photobleaching

Influence of sensor composition on nanoparticle and protein interaction with supported lipid bilayers

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