Formaldehyde lacks genotoxicity in formaldehyde-hyperresistant strains of the yeast Saccharomyces cerevisiae

Wehner, Eugen; Brendel, Martin

doi:10.1016/0027-5107(93)90134-2

Cited by 5 publications

(2 citation statements)

References 25 publications

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“…Although the signal ratios of the SFA1 homolog (Wehner and Brendel, 1993; AO080572000002) and the GSH1 homolog (Ohtake and Yabuuchi, 1991; AO080537000068) versus the control strain were not decreased in the DatfB strain, they were much reduced in the DatfA strain. These genes would explain why the DatfA conidia were more sensitive to oxidative stress than the DatfB conidia.…”

Section: Gene Expression Profile Of Colony Surfacementioning

confidence: 93%

Aspergillus oryzae atfA controls conidial germination and stress tolerance

Sakamoto

Iwashita

Kobayashi

et al. 2009

Fungal Genetics and Biology

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Section: Gene Expression Profile Of Colony Surfacementioning

confidence: 93%

Aspergillus oryzae atfA controls conidial germination and stress tolerance

Sakamoto

Iwashita

Kobayashi

et al. 2009

Fungal Genetics and Biology

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“…When the desired production gene has been cloned into our vectors, the E. coli pBR322-derived ampicillin resistance marker should be eliminated so that antibiotic resistant markers would not be spread in the product or in the waste; also, elimination of pBR322 sequences would reduce vector size resulting in larger copy numbers in the transformants. In 1993, we also showed that the genotoxicity of the selective chemical FA is drastically reduced in FA-hyperresistant yeast cells so that stability of plasmid constructions is insured (21). Although the efficiency of the lithium acetate transformation procedure is sufficient to obtain transformants of most yeast strains used in this study, one strain could only be transformed by applying the more time-consuming spheroplast protocol.…”

Section: Discussionmentioning

confidence: 87%

Genetic engineering of baker's and wine yeasts using formaldehyde hyperresistance-mediating plasmids

Schmidt

Comer

Grey

et al. 1997

Braz J Med Biol Res

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Yeast multi-copy vectors carrying the formaldehyde-resistance marker gene SFA have proved to be a valuable tool for research on industrially used strains of Saccharomyces cerevisiae. The genetics of these strains is often poorly understood, and for various reasons it is not possible to simply subject these strains to protocols of genetic engineering that have been established for laboratory strains of S. cerevisiae. We tested our vectors and protocols using 10 randomly picked baker's and wine yeasts all of which could be transformed by a simple protocol with vectors conferring hyperresistance to formaldehyde. The application of formaldehyde as a selecting agent also offers the advantage of its biodegradation to CO 2 during fermentation, i.e., the selecting agent will be consumed and therefore its removal during down-stream processing is not necessary. Thus, this vector provides an expression system which is simple to apply and inexpensive to use.

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Vector YFRp1 allows transformant selection in Saccharomyces cerevisiae via resistance to formaldehyde

Wehner¹,

Brendel²

1993

Yeast

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Formaldehyde (FA), a chemical with low toxic potential, is used as sole selective agent for transformation in the yeast Saccharomyces cerevisiae. Neither stable auxotrophic markers in recipient cells nor defined synthetic media are needed when multicopy vector YFRp1, containing the yeast SFA gene, is employed for yeast transformation. The SFA gene gives stability to the vector and its yeast (and other) passenger genes when transformants are propagated in complex media supplemented with 3-5 mM-FA. Use of inexpensive FA and non-synthetic, undefined media will lower the cost of yeast transformant propagation considerably and thus make feasible large-volume industrial application of transformants containing YFRp1 derivatives.

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Formaldehyde lacks genotoxicity in formaldehyde-hyperresistant strains of the yeast Saccharomyces cerevisiae

Cited by 5 publications

References 25 publications

Aspergillus oryzae atfA controls conidial germination and stress tolerance

Aspergillus oryzae atfA controls conidial germination and stress tolerance

Genetic engineering of baker's and wine yeasts using formaldehyde hyperresistance-mediating plasmids

Vector YFRp1 allows transformant selection in Saccharomyces cerevisiae via resistance to formaldehyde

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