Formal honeybush tea industry reaches 20-year milestone – progress of product research targeting phenolic composition, quality and bioactivity

Joubert, Elizabeth; Beer, Dalene de; Malherbe, Christiaan J.; Müller, M.; Louw, Ann; Gelderblom, Wentzel C. A.

doi:10.1016/j.sajb.2019.08.027

Cited by 35 publications

(35 citation statements)

References 93 publications

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“…Nowadays, special attention is paid to the use of honeybush as a phytotherapeutic agent, and about half of the related literature has been published in the last five years. Thus, for instance, the antioxidant, antimicrobial, and antimutagenic activities of honeybush have been tested in different cell types, with promising results [3,4]. However, the effects of honeybush on the reproductive function are still unknown, both in vivo and in vitro.…”

Section: Introductionmentioning

confidence: 99%

“…The main goal of the present study was to evaluate the effects of fermented honeybush (Cyclopia intermedia) extract on samples of diluted boar semen. Because of its antioxidant properties [4], we hypothesized that the addition of honeybush extract to a semen extender preserves sperm quality under oxidative stress and prolongs sperm lifespan during semen storage. In the first experiment, in order to evaluate the potential of honeybush as an ROS scavenger, diluted semen samples were submitted to oxidative stress using Fe 2+ /ascorbate as an ROS inductor (hydroxyl radical, • OH) that promotes lipid peroxidation.…”

Section: Introductionmentioning

confidence: 99%

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Pharmacological Activity of Honeybush (Cyclopia intermedia) in Boar Spermatozoa during Semen Storage and under Oxidative Stress

Ros-Santaella

Kadlec

Pintus

2020

Animals

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In recent decades, an increasing number of ethnopharmacological studies have been dedicated to medicinal plants from South African fynbos. Among these plants, honeybush (Cyclopia spp.) has become a popular tea, mainly due to its healthy properties and caffeine-free status. The antioxidant, antimutagenic, and antimicrobial properties of this plant have been reported in several cell types, but its effects on reproductive function are still unknown. Here, we assessed the effects of honeybush (Cyclopia intermedia) on boar sperm parameters under induced oxidative stress (Fe2+/ascorbate) and during five days of semen storage at 17 °C without oxidative stress. In both experiments, four concentrations (200, 50, 12.5, and 3.125 µg/mL) of fermented honeybush were tested. Our results show that honeybush enhances sperm parameters, and no toxic effects were observed at any of the tested extract concentrations. Interestingly, honeybush (12.5 µg/mL) improved the sperm motility and kinetic parameters, preserved the plasma membrane integrity, and reduced the lipid peroxidation in the samples exposed to Fe2+/ascorbate (p < 0.05). In the stored samples, positive effects of honeybush on sperm parameters (motility, kinetics, acrosome, and mitochondria) were observed from 48 h until 120 h of semen storage (p < 0.05). Our results clearly show the protective effects of honeybush on sperm samples, thus promoting its use as a natural source of antioxidants for boar semen.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pharmacological Activity of Honeybush (Cyclopia intermedia) in Boar Spermatozoa during Semen Storage and under Oxidative Stress

Ros-Santaella

Kadlec

Pintus

2020

Animals

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“…The pharmacological activity of Cyclopia spp. is caused by the synergic effect of polyphenolic fraction-xanthones, flavanones, flavones, isoflavones, coumestans, phenolic acids, and others [20]. Therefore, extracts from honeybush have strong antioxidant properties with a large amount of mangiferin and hesperidin, compounds well known as oxidative stress fighters [17,[20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

“…is caused by the synergic effect of polyphenolic fraction-xanthones, flavanones, flavones, isoflavones, coumestans, phenolic acids, and others [20]. Therefore, extracts from honeybush have strong antioxidant properties with a large amount of mangiferin and hesperidin, compounds well known as oxidative stress fighters [17,[20][21][22][23][24][25][26]. These compounds, among others, are also responsible for the skin UV protection [20,27] and inhibition of wrinkle formation [28].…”

Section: Introductionmentioning

confidence: 99%

Mangiferin and Hesperidin Transdermal Distribution and Permeability through the Skin from Solutions and Honeybush Extracts (Cyclopia sp.)—A Comparison Ex Vivo Study

et al. 2021

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Polyphenolic compounds—mangiferin and hesperidin—are, among others, the most important secondary metabolites of African shrub Cyclopia sp. (honeybush). The aim of this study was to compare the percutaneous absorption of mangiferin and hesperidin from solutions (water, ethanol 50%, (v/v)) and extracts obtained from green and fermented honeybush (water, ethanol 50%, (v/v)). Research was performed with the Bronaugh cells, on human dorsal skin. The mangiferin and hesperidin distributions in skin layers (stratum corneum, epidermis, and dermis) and in acceptor fluid (in every 2, 4, 6, and 24 h) were evaluated by HPLC–Photodiode Array Coulometric and Coulometric Electrochemical Array Detection. The transdermal distribution of hesperidin was also demonstrated by fluorescence microscopy. Results indicated that mangiferin and hesperidin were able to cross the stratum corneum and penetrate into the epidermis and dermis. An advantage of hesperidin penetration into the skin from the water over ethanol solution was observed (451.02 ± 14.50 vs. 357.39 ± 4.51 ng/cm2), as well as in the mangiferin study (127.56 ± 9.49 vs. 97.23 ± 2.92 ng/cm2). Furthermore, mangiferin penetration was more evident from nonfermented honeybush ethanol extract (189.85 ± 4.11 ng/cm2) than from solutions. The permeation of mangiferin and hesperidin through the skin to the acceptor fluid was observed regardless of whether the solution or the honeybush extract was applied. The highest ability to permeate the skin was demonstrated for the water solution of hesperidin (250.92 ± 16.01 ng/cm2), while the hesperidin occurring in the extracts permeated in a very low capacity. Mangiferin from nonfermented honeybush ethanol extract had the highest ability to permeate to the acceptor fluid within 24 h (152.36 ± 8.57 ng/cm2).

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“…In this context, several studies on Cyclopia spp. linked putative bioactivity to specific polyphenols present in their extracts with the focus largely on the major compounds while minor compounds have been largely ignored as recently reviewed 4 . However, minor compounds could also play an integral role in health‐promoting properties of plant extracts, 5 as they may be highly potent, 6 highlighting the need to fill the gap in our knowledge of the phenolic composition of Cyclopia spp.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive off‐line CCC × LC‐DAD‐MS separation of Cyclopia pubescens Eckl. & Zeyh. phenolic compounds and structural elucidation of isolated compounds

Walters

Beer

Villiers

et al. 2020

Phytochemical Analysis

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Introduction The minor phenolic constituents of Cyclopia pubescens Eckl. & Zeyh. are unknown and one dimensional (1D) liquid chromatography (LC) is unable to provide sufficient separation. Methodology A two‐dimensional (2D) LC method incorporating normal‐phasehigh performance countercurrent chromatography (NP‐HPCCC) in the first dimension (1D) and reversed‐phase ultra‐high‐performance liquid chromatography (RP‐UHPLC) as the second dimension (2D) was developed. The analytical HPCCC method was subsequently scaled up to semi‐preparative mode and fractions pooled based on phenolic sub‐groups. The phenolic compounds in selected fractions were subsequently isolated using RP‐HPLC on a C18 column. Isolated compounds were identified by nuclear magnetic resonance (NMR) spectroscopy. The absolute configurations of compounds were determined by optical rotation and electronic circular dichroism spectra. Sugars were identified by gas chromatography‐mass spectrometry (GC–MS) analysis. Results The comprehensive off‐line 2D CCC × LC method gave a good spread of the phenolic compounds. Orthogonality calculated using both the convex hull and conditional entropy methods were 81%. High‐resolution mass spectrometric fragmentation spectra obtained from a quadrupole‐time‐of‐flight instrument and ultraviolet‐visible (UV‐vis) spectral data were used to (tentatively) identify 32 phenolic compounds from the analytical CCC fractions. Of the seven isolated compounds, (2S)‐5‐O‐[α‐l‐rhamnopyranosyl‐(1 → 2)‐β‐d‐glucopyranosyl]eriodictyol (3) and (2S)‐5‐O‐[α‐l‐rhamnopyranosyl‐(1 → 2)‐β‐d‐glucopyranosyl]‐5,7,3′,4′‐tetrahydroxyflavan (4) were newly identified in all plants. The other isolated compounds were identified as (2S)‐5‐O‐[α‐l‐rhamnopyranosyl‐(1 → 2)‐β‐d‐glucopyranosyl]naringenin (1), R‐neo‐eriocitrin (2), 3‐O‐α‐l‐arabinopyranosyl‐3,4‐dihydroxybenzoic acid (5), 4‐O‐β‐d‐glucopyranosyl‐Z‐4‐hydroxycinnamic acid (6) and 4‐(4′‐O‐β‐d‐glucopyranosyl‐4′‐hydroxy‐3′‐methoxyphenyl)‐2‐butanone (7). Conclusions Among the 32 compounds (tentatively) identified, only six were previously identified in Cyclopia pubescens using 1D LC. Most of the isolated compounds were also identified for the first time in Cyclopia spp., improving the knowledge of the minor phenolic compounds of this genus.

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Formal honeybush tea industry reaches 20-year milestone – progress of product research targeting phenolic composition, quality and bioactivity

Cited by 35 publications

References 93 publications

Pharmacological Activity of Honeybush (Cyclopia intermedia) in Boar Spermatozoa during Semen Storage and under Oxidative Stress

Pharmacological Activity of Honeybush (Cyclopia intermedia) in Boar Spermatozoa during Semen Storage and under Oxidative Stress

Mangiferin and Hesperidin Transdermal Distribution and Permeability through the Skin from Solutions and Honeybush Extracts (Cyclopia sp.)—A Comparison Ex Vivo Study

Comprehensive off‐line CCC × LC‐DAD‐MS separation of Cyclopia pubescens Eckl. & Zeyh. phenolic compounds and structural elucidation of isolated compounds

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