Formación de biopelículas por Listeria monocytogenes aisladas de queso fresco de mercados del Cercado de Lima

Abstract

Resultados. Se identificó L. monocytogenes en 14 muestras (18,7%), valor que representó un riesgo potencial para la salud de los consumidores. Se halló nueve cepas con capacidad de formar biopelículas que, según la densidad óptica a 595 nm, fueron clasificadas en formadoras débiles y moderadas, con diferencias significativas dependiendo del medio de cultivo utilizado, siendo el caldo infusión cerebro corazón (BHI) el más efectivo para esta bacteria. Conclusiones. Se identificó L. monocytogenes en 18,7% (14/75) de las muestras de quesos frescos adquiridas en 10 mercados del Cercado de Lima. De estas cepas, 64,3% (9/14) tuvieron capacidad formadora de biopelículas. Palabras clave. Listeria monocytogenes; Queso; Biopelículas. Abstract Introduction: L. monocytogenes is a bacterium of importance in public health because it causes listeriosis, which has serious consequences. It is associated with the consumption of foods that can be contaminated during its production with bacteria released from pipes, surfaces and equipment biofilms. Objectives: To analyze cheese samples in order to isolate and identify L. monocytogenes and evaluate its ability for biofilm formation in two culture media. Design. Experimental, prospective study. Setting:

Microbial study of household hygiene conditions and associated Listeria monocytogenes infection risks for Peruvian women

“…A few studies, however, have explored L. monocytogenes in the context of local Peruvian food and public health. For example, a 2017 study sampled 75 samples of fresh cheese across 10 markets in Lima and found 18.7% positive for L. monocytogenes [3]. Previous studies in Ica and Trujillo found smaller percentages, 4.05% and 3.34%, respectively, for unpasteurized cheese products [4,5].…”

Review of CRISPR-Cas Systems in Listeria Species: Current Knowledge and Perspectives

“…The role of the biofilm as a pathogen and persistent mechanism for L. monocytogenes has been described [ 6 , 43 – 48 ]. The ability to proliferate in cold and humid environments, as well as the ease of adherence to surfaces, makes L. monocytogenes capable of forming biofilms on materials such as plastics, metals, and food [ 49 ]. Moreover, the CRISPR-Cas systems could be involved in the regulation of virulence gene expression [ 25 , 26 ], and it would be interesting to elucidate the relationship between the presence of CRISPR in Listeria and the biofilm formation capacity of this bacterium in different materials (for example: prosthetic materials and FPEs) [ 50 – 53 ].…”