Form, Function, and Regulation of ARGONAUTE Proteins

Mallory, Allison C.; Vaucheret, Hervé

doi:10.1105/tpc.110.080671

Cited by 364 publications

(348 citation statements)

References 92 publications

Supporting

Mentioning

316

Contrasting

Unclassified

Order By: Relevance

“…This result suggested that translated ORFs were present upstream of the noncleavable target site of miR390 and 59-truncated RNA ends mapped to this region were likely ribosome footprints. If these PARE peaks represent the footprints of ribosomes hindered by miR390-AGO7 complex but not the cleavage remnants directed by tasiRNAs, they should disappear in the ago7 but be sustained in the mutant of RNA-DEPENDENT RNA POLYMERASE6 (RDR6), which acts downstream of AGO7 in the tasiRNA biogenesis pathway (Mallory and Vaucheret, 2010). As predicted, the result of modified RLM 59 RACE clearly showed that degradation fragments with 59 ends mapped to these positions were barely detected in ago7 ( Figure 8B).…”

Section: Discovery Of Potential Footprints Of Ribosomes Hindered By Amentioning

confidence: 99%

Global Analysis of Truncated RNA Ends Reveals New Insights into Ribosome Stalling in Plants

et al. 2016

View full text Add to dashboard Cite

High-throughput approaches for profiling the 59 ends of RNA degradation intermediates on a genome-wide scale are frequently applied to analyze and validate cleavage sites guided by microRNAs (miRNAs). However, the complexity of the RNA degradome other than miRNA targets is currently largely uncharacterized, and this limits the application of RNA degradome studies. We conducted a global analysis of 59-truncated mRNA ends that mapped to coding sequences (CDSs) of Arabidopsis thaliana, rice (Oryza sativa), and soybean (Glycine max). Based on this analysis, we provide multiple lines of evidence to show that the plant RNA degradome contains in vivo ribosome-protected mRNA fragments. We observed a 3-nucleotide periodicity in the position of free 59 RNA ends and a bias toward the translational frame. By examining conserved peptide upstream open reading frames (uORFs) of Arabidopsis and rice, we found a predominance of 59 termini of RNA degradation intermediates that were separated by a length equal to a ribosome-protected mRNA fragment. Through the analysis of RNA degradome data, we discovered uORFs and CDS regions potentially associated with stacked ribosomes in Arabidopsis. Furthermore, our analysis of RNA degradome data suggested that the binding of Arabidopsis ARGONAUTE7 to a noncleavable target site of miR390 might directly hinder ribosome movement. This work demonstrates an alternative use of RNA degradome data in the study of ribosome stalling.

show abstract

Section: Discovery Of Potential Footprints Of Ribosomes Hindered By Amentioning

confidence: 99%

Global Analysis of Truncated RNA Ends Reveals New Insights into Ribosome Stalling in Plants

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Arabidopsis thaliana encodes 10 AGO proteins that are thought to be specialized to function in different RNA silencingrelated mechanisms (Mallory and Vaucheret, 2010), some of which have been implicated in antiviral defense. Hypomorphic ago1 mutants have increased susceptibility to Brome mosaic virus and Cucumber mosaic virus (CMV) and to VSR-defective CMV and Turnip crinkle virus (TCV) (Morel et al, 2002;Qu et al, 2008;Azevedo et al, 2010;Dzianott et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Functional and Genetic Analysis Identify a Role for Arabidopsis ARGONAUTE5 in Antiviral RNA Silencing

2015

View full text Add to dashboard Cite

RNA silencing functions as an antiviral defense through the action of DICER-like (DCL) and ARGONAUTE (AGO) proteins. In turn, plant viruses have evolved strategies to counteract this defense mechanism, including the expression of suppressors of RNA silencing. Potato virus X (PVX) does not systemically infect Arabidopsis thaliana Columbia-0, but is able to do so effectively in mutants lacking at least two of the four Arabidopsis DCL proteins. PVX can also infect Arabidopsis ago2 mutants, albeit less effectively than double DCL mutants, suggesting that additional AGO proteins may mediate anti-viral defenses. Here we show, using functional assays, that all Arabidopsis AGO proteins have the potential to target PVX lacking its viral suppressor of RNA silencing (VSR), P25, but that only AGO2 and AGO5 are able to target wild-type PVX. However, P25 directly affects only a small subset of AGO proteins, and we present evidence indicating that its protective effect is mediated by precluding AGO proteins from accessing viral RNA, as well as by directly inhibiting the RNA silencing machinery. In agreement with functional assays, we show that Potexvirus infection induces AGO5 expression and that both AGO2 and AGO5 are required for full restriction of PVX infection in systemic tissues of Arabidopsis.

show abstract

“…Docking was performed between AGO protein and miRNA-mRNA duplexes. In the process of miRNAs mediated gene regulation, the Argonaute (AGO) [14,15] class of proteins play a significant role in the hybrid formation between miRNA-mRNA. …”

Section: Prediction Of Mirna and Targets Genesmentioning

confidence: 99%

Study of miRNA based gene regulation involved in Squamous Lung Carcinoma by assistance of Argonaute Protein

Chandra¹,

Kumar²,

Khan³

2018

IJRDPL

View full text Add to dashboard Cite

miRNAs are responsible for gene silencing and has functions in various diseases like cancer. miRNAs are responsible for cancer and at the same time act as tumor suppressors. The work mentioned here is based on study on differentially expressed miRNAs for squamous cell carcinoma target genes in the metabolic pathways. The study focused on combination of miRNA and their target genes for squamous cell carcinoma as well as how they are involved in metabolic pathways. miRNAs were categorized into normal, up-regulated and down-regulated genes. Prediction of target genes for miRNAs followed by developing a relationship between them was done. miRNA target genes involved in metabolic pathways which were studied for squamous lung carcinoma. miRNA target genes were doc ked with argonaute protein. Docking was done in order to understand how miRNA tend to bind to target gene. Results added information to the study of how miRNAs targets genes in various metabolic pathways. The expression level of miRNA is different in diffe rent conditions which can be modified as a result of defects in the miRNA biogenesis pathway.

show abstract

Form, Function, and Regulation of ARGONAUTE Proteins

Cited by 364 publications

References 92 publications

Global Analysis of Truncated RNA Ends Reveals New Insights into Ribosome Stalling in Plants

Global Analysis of Truncated RNA Ends Reveals New Insights into Ribosome Stalling in Plants

Functional and Genetic Analysis Identify a Role for Arabidopsis ARGONAUTE5 in Antiviral RNA Silencing

Study of miRNA based gene regulation involved in Squamous Lung Carcinoma by assistance of Argonaute Protein

Contact Info

Product

Resources

About