The utilization of natural resources should consider the availability and the carrying capacity of the nature for the society’s prosperity purpose since the excessive use of natural resources can lead to any disasters. The purpose of this study is to find out and analyze the implementation of conservation policy of natural resources and ecosystems in Kuningan. The method used was mixed method and the data collection techniques were interview, observation, and literature study. The results revealed that the conservation policy of natural resources is regulated based on Law Number 5 of 1990 about The Conservation of Natural Resources and Ecosystems, and other related Regulations such as Government Regulation, Ministerial Regulation, and Regional Regulation. Further, regarding the implementation of conservation policy of natural resources and ecosystems in Kuningan, the regional government stipulated the protected forest area of Mount Ciremai to become The Mount Ciremai National Park in 2004 and created regional regulations concerning the conservation of water resources, botanical gardens, and bird’s protection. In conclusion, the implementation of conservation policy of natural resources and ecosystems in Kuningan is running well. Yet, the government’s consistency in conducting programs related to the conservation of natural resources and ecosystems becomes the future challenge.