Forensic Science

Brettell, Thomas A.; Butler, John M.; Almirall, José R.

doi:10.1021/ac201075e

Cited by 58 publications

(32 citation statements)

References 554 publications

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“…Forensic analysis is a rapidly growing area of bioanalytical chemistry [1]. In particular, DNA typing has been considerably developed as the most common method for identifying individuals.…”

Section: Introductionmentioning

confidence: 99%

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Identification and evaluation of potential forensic marker proteins in vaginal fluid by liquid chromatography/mass spectrometry

2015

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Vaginal fluid is one of the most common body fluids found at crime scenes. Discriminating vaginal fluid from other body fluids is important in forensic science; however, few potential protein markers have been reported to date. Proteomic methods for identifying protein markers have gained attention, although few reports have applied this technology to forensic protein markers. Therefore, to identify characteristic vaginal proteins, we examined various body fluids (nasal secretions, saliva, urine, semen, vaginal fluids, and sweat) using liquid chromatography/electrospray ionization time-of-flight mass spectrometry and peptide mass fingerprinting. We identified three components (average molecular mass values 17,237 ± 2, 18,063 ± 2, and 15,075 ± 1) detectable only in vaginal samples: two human small proline-rich protein 3 (SPRR3) isoforms and a human fatty acid-binding protein 5 (FABP5) with an acetylated (+42) N-terminal region lacking the initiator methionine residue (-131). Using ELISA, these yielded markedly high average values in vaginal fluids. The mass spectra of these proteins were not detected in infant saliva but were detected in the vaginal fluid throughout the menstrual cycle. The results of forensic analysis (detection limit, mixed body fluid samples, casework samples, and blind samples) suggest that these proteins are potential forensic markers. In conclusion, high SPRR3 and FABP5 expression levels, which may be used as potential markers for vaginal fluid identification in forensic science, were detected in vaginal fluids from healthy adults.

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Section: Introductionmentioning

confidence: 99%

“…Mass spectrometry is also used for other purposes in forensic institutes, such as identifying drugs and poisons in forensic toxicology [1], but not for the analysis of biomaterials in body fluids. The potential application of mass spectrometry in forensic biomaterial analysis has been described only in a few recent reports [7,13,14].…”

Section: Introductionmentioning

confidence: 99%

Identification and evaluation of potential forensic marker proteins in vaginal fluid by liquid chromatography/mass spectrometry

2015

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“…Other review articles provide a more comprehensive and objective discussion of research articles in different areas of forensic chemistry (2,3). This review intends to provide an unbiased but critical discussion of the status of forensic mass spectrometry (MS) research, and not at all an encyclopedic coverage of all the papers in the field.…”

Section: Introductionmentioning

confidence: 99%

Forensic Mass Spectrometry

Hoffmann¹,

Jackson

2015

Annual Rev. Anal. Chem.

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Developments in forensic mass spectrometry tend to follow, rather than lead, the developments in other disciplines. Examples of techniques having forensic potential born independently of forensic applications include ambient ionization, imaging mass spectrometry, isotope ratio mass spectrometry, portable mass spectrometers, and hyphenated chromatography-mass spectrometry instruments, to name a few. Forensic science has the potential to benefit enormously from developments that are funded by other means, if only the infrastructure and personnel existed to adopt, validate, and implement the new technologies into casework. Perhaps one unique area in which forensic science is at the cutting edge is in the area of chemometrics and the determination of likelihood ratios for the evaluation of the weight of evidence. Such statistical techniques have been developed most extensively for ignitable-liquid residue analyses and isotope ratio analysis. This review attempts to capture the trends, motivating forces, and likely impact of developing areas of forensic mass spectrometry, with the caveat that none of this research is likely to have any real impact in the forensic community unless: (a) The instruments developed are turned into robust black boxes with red and green lights for positives and negatives, respectively, or (b) there are PhD graduates in the workforce who can help adopt these sophisticated techniques.

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“…Detection of deoxyribonucleic acids (DNA) is essential for the analysis of pathogens in food [1,2], forensic science [3], and clinical diagnosis [4,5]. Often, DNA assays are carried out in a microarrayed chip format allowing for parallel analysis at a high number of test sites [6][7][8].…”

Section: Introductionmentioning

confidence: 99%