Forensic quest for age determination of bloodstains

Bremmer, Rolf H.; Bruin, Karla G. de; Gemert, Martin J. C. van; Leeuwen, Ton G. van; Aalders, Maurice C. G.

doi:10.1016/j.forsciint.2011.07.027

Cited by 125 publications

(119 citation statements)

References 61 publications

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“…However, other biological molecules may also offer opportunities for conservation and research. For example, RNA is generally less stable than DNA, and for this reason its degradation has been used by forensic scientists to estimate the time since deposition of biological material (Bremmer et al 2012). Therefore, assays targeting eRNA may provide narrower spatiotemporal inferences of organism presence or abundance than eDNA.…”

Section: Isolating Molecules Other Than Dna From Environmental Samplesmentioning

confidence: 99%

The ecology of environmental DNA and implications for conservation genetics

2015

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Environmental DNA (eDNA) refers to the genetic material that can be extracted from bulk environmental samples such as soil, water, and even air. The rapidly expanding study of eDNA has generated unprecedented ability to detect species and conduct genetic analyses for conservation, management, and research, particularly in scenarios where collection of whole organisms is impractical or impossible. While the number of studies demonstrating successful eDNA detection has increased rapidly in recent years, less research has explored the ''ecology'' of eDNA-myriad interactions between extraorganismal genetic material and its environment-and its influence on eDNA detection, quantification, analysis, and application to conservation and research. Here, we outline a framework for understanding the ecology of eDNA, including the origin, state, transport, and fate of extraorganismal genetic material. Using this framework, we review and synthesize the findings of eDNA studies from diverse environments, taxa, and fields of study to highlight important concepts and knowledge gaps in eDNA study and application. Additionally, we identify frontiers of conservation-focused eDNA application where we see the most potential for growth, including the use of eDNA for estimating population size, population genetic and genomic analyses via eDNA, inclusion of other indicator biomolecules such as environmental RNA or proteins, automated sample collection and analysis, and consideration of an expanded array of creative environmental samples. We discuss how a more complete understanding of the ecology of eDNA is integral to advancing these frontiers and maximizing the potential of future eDNA applications in conservation and research.

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Section: Isolating Molecules Other Than Dna From Environmental Samplesmentioning

confidence: 99%

The ecology of environmental DNA and implications for conservation genetics

2015

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“…Bremmer et al (2012) provide an informative review about this issue and discuss approaches such as the detection of haem degradation or loss of enzyme activity that have been explored in the past. The authors conclude that further improvement and more information on environmental factors are required prior to forensic implementation.…”

Section: Body Fluid Identification Methods: Current and Applications mentioning

confidence: 99%

Forensic DNA Analysis

Prinz¹,

Lessig²

2014

Handbook of Forensic Medicine

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“…Several chemical, physical or optical methods have recently been developed in BPA for detecting weakly visible stains, using functionalized nanoparticles [158] or Raman scattering [159], and for determining the age of bloodstains, as reviewed by Bremmer et al [160].…”

Section: Description In the Bpa Literaturementioning

confidence: 99%

Fluid dynamics topics in bloodstain pattern analysis: Comparative review and research opportunities

Attinger

Moore

Donaldson

et al. 2013

Forensic Science International

151

139

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This comparative review highlights the relationships between the disciplines of bloodstain pattern analysis (BPA) in forensics and that of fluid dynamics (FD) in the physical sciences. In both the BPA and FD communities, scientists study the motion and phase change of a liquid in contact with air, or with other liquids or solids. Five aspects of BPA related to FD are discussed: the physical forces driving the motion of blood as a fluid; the generation of the drops; their flight in the air; their impact on solid or liquid surfaces; and the production of stains. For each of these topics, the relevant literature from the BPA community and from the FD community is reviewed. Comments are provided on opportunities for joint BPA and FD research, and on the development of novel FD-based tools and methods for BPA. Also, the use of dimensionless numbers is proposed to inform BPA analyses. Keywordsbloodstain pattern analysis, review, dimensionless number, drop generation, trajectory, impact, stain Disciplines Laboratory and Basic Science Research | Other Mechanical EngineeringComments NOTICE: this is the author's version of a work that was accepted for publication in Forensic Science International. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Forensic Science International, [231, 1-3, (2013) Abstract: This comparative review highlights the relationships between the disciplines of bloodstain pattern analysis (BPA) in forensics and that of fluid dynamics (FD) in the physical sciences. In both the BPA and FD communities, scientists study the motion and phase change of a liquid in contact with air, or with other liquids or solids. Five aspects of BPA related to FD are discussed: the physical forces driving the motion of blood as a fluid; the generation of the drops; their flight in the air; their impact on solid or liquid surfaces; and the production of stains. For each of these topics, the relevant literature from the BPA community and from the FD community is reviewed. Comments are provided on opportunities for joint BPA and FD research, and on the development of novel FD-based tools and methods for BPA. Also, the use of dimensionless numbers is proposed to inform BPA analyses.

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Forensic quest for age determination of bloodstains

Cited by 125 publications

References 61 publications

The ecology of environmental DNA and implications for conservation genetics

The ecology of environmental DNA and implications for conservation genetics

Forensic DNA Analysis

Fluid dynamics topics in bloodstain pattern analysis: Comparative review and research opportunities

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