Forensic identification of urine using the DMAC test: A method validation study

Ong, Sandy Y.; Wain, Adrian; Groombridge, Linda; Grimes, Eileen

doi:10.1016/j.scijus.2011.07.007

Cited by 10 publications

(4 citation statements)

References 12 publications

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“…In acidic conditions, DMAC specifically reacts with meta -diphenols to form a green carbonium ion detected at 640 nm. The DMAC dosage is as highly specific to Pcys as it does not react with other flavonoids like anthocyanins [ 44 ]. However, the colour development depends on the procyanidin structural conformation, and although it has not been demonstrated yet, several authors have suggested that the DMAC reagent could react with only one flavan-3-ol monomer inside a Pcys [ 45 , 46 ] leading us to an underestimation of Pcy contents, especially for polymers.…”

Section: Methodsmentioning

confidence: 99%

Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer

Minker

Duban

Karaś

et al. 2015

Oxidative Medicine and Cellular Longevity

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Scope. The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries. Methods and Results. Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells. Conclusion. We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

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Section: Methodsmentioning

confidence: 99%

Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer

Minker

Duban

Karaś

et al. 2015

Oxidative Medicine and Cellular Longevity

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“…After confirming that the spots were urine spots by the DMAC (p-dimethylaminocinnamaldehyde) method [6] (Figure 1), the DNA typing of the urine spots was performed using GlobalFiler™ [7].…”

Section: A Sementioning

confidence: 99%

“…After confirming that the spots were urine spots by the DMAC (p‐dimethylaminocinnamaldehyde) method [6] (Figure 1), the DNA typing of the urine spots was performed using GlobalFiler™ [7]. However, only seven loci could be determined to be identical to the missing woman's DNA (which was obtained from the rib).…”

Section: Casementioning

confidence: 99%

Detection of human chorionic gonadotropin (HCG) in fabric urine stains older than five years

Minamiyama,

Kawamoto,

Matsunari

et al. 2023

Journal of Forensic Sciences

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Human chorionic gonadotropin (HCG) is used in clinical medicine as a particularly important indicator to determine pregnancy. In this study, it was necessary to determine whether the urine spots on car seat fabric from a murder 5 years previously were from a pregnant woman. The HCG in the dried urine spot on a car seat was detected using an immunochromatography kit. It was found that the HCG in urine can be detected for much longer periods of time than the previously reported period of approximately 6 months.

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“…In both assays, the extracts with the lowest PA values were obtained with the NaDES ChCl:EG, ChCl:sorb, and ChCl:urea. With ChCl:urea, no results could be obtained for the total PA content with DMAC assay because DMAC reacts with urea, this being a method that is also used to detect the presence of urine in samples [53]. Therefore, more-polar NaDES composed of organic acids such as lactic acid, formic acid, and citric acid extracted greater amounts of PAs than less-polar NaDES based on sugar and polyalcohol such as the NaDES composed of sorbitol [54].…”

Section: Nades Screening For the Extraction Of Neps From Mangosteen Peelmentioning

confidence: 99%

A Sustainable Approach for Extracting Non-Extractable Phenolic Compounds from Mangosteen Peel Using Ultrasound-Assisted Extraction and Natural Deep Eutectic Solvents

et al. 2021

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Mangosteen (Garcinia mangostana L.) peel is a potential source of phenolic compounds with beneficial properties. Natural deep eutectic solvents (NaDES) have been considered an environmentally friendly and cheap alternative to conventional organic solvents. In this work, a green extraction methodology was developed using ultrasound-assisted extraction (UAE) and NaDES for the extraction of antioxidant non-extractable polyphenols (NEPs) from mangosteen peel. To select the best NaDES to extract NEPs from mangosteen peel, seven NaDES were studied. Antioxidant capacity and total phenolic and proanthocyanidin contents were determined for the extracts. The molecular weights for the NEPs present in those extracts were evaluated by size exclusion chromatography. Experimental results showed that choline chloride–lactic acid (1:2) was the NaDES allowing the highest antioxidant proanthocyanidin content in the extracts. A Box–Behnken experimental design was employed to optimize the main parameters in UAE with NaDES: water percentage, ultrasound amplitude, and extraction time. The optimal extraction conditions were 18.8% (v/v) water, 60% ultrasound amplitude, and 15 min as the extraction time. In addition, the cytotoxicity of the NEP extracts obtained under optimal extraction conditions was evaluated. Results indicated for the first time that the use of NaDES in combination with UAE could be a sustainable alternative for the extraction of antioxidant NEPs from mangosteen peel for important applications in the food, pharmaceutical, agrochemical, and cosmetic fields, as the extracts presented low cytotoxicity.

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Forensic identification of urine using the DMAC test: A method validation study

Cited by 10 publications

References 12 publications

Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer

Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer

Detection of human chorionic gonadotropin (HCG) in fabric urine stains older than five years

A Sustainable Approach for Extracting Non-Extractable Phenolic Compounds from Mangosteen Peel Using Ultrasound-Assisted Extraction and Natural Deep Eutectic Solvents

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