Forensic identification of an individual in complex DNA mixtures

Voskoboinik, Lev; Darvasi, Ariel

doi:10.1016/j.fsigen.2010.09.002

Cited by 49 publications

(42 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Panels of SNPs can be specifically designed to identify multiple individuals in complex DNA samples [1][2][3][4] . Isaacson et al 2 introduced SNP panel mixture analysis with drop-in and drop-out alleles present with confidence calculations for detecting individuals in mixtures.…”

Section: Introductionmentioning

confidence: 99%

“…Isaacson et al 2 introduced SNP panel mixture analysis with drop-in and drop-out alleles present with confidence calculations for detecting individuals in mixtures. The confidence with which an individual can be identified in a DNA mixture using a given SNP panel is dependent upon the number of SNP loci in the panel and the average population ratio of minor to major SNP alleles 1,2 . Ricke and Schwartz 5 refined the calculations for larger HTS SNP panels.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

TranslucentID: Detecting Individuals with High Confidence in Saturated DNA SNP Mixtures

Ricke

Watkins

Fremont-Smith

et al. 2018

Preprint

View full text Add to dashboard Cite

High throughput sequencing (HTS) of complex DNA mixtures with single nucleotide polymorphisms (SNPs) panels can identify multiple individuals in forensic DNA mixture samples. SNP mixture analysis relies upon the exclusion of non-contributing individuals with the subset of SNP loci with no detected minor alleles in the mixture. Few, if any, individuals are anticipated to be detectable in saturated mixtures by this mixture analysis approach because of the increased probability of matching random individuals. Being able to identify a subset of the contributors in saturated HTS SNP mixtures is valuable for forensic investigations. A desaturated mixture can be created by treating a set of SNPs with the lowest minor allele ratios as having no minor alleles. Leveraging differences in DNA contributor concentrations in saturated mixtures, we introduce TranslucentID for the identification of a subset of individuals with high confidence who contributed DNA to saturated mixtures by desaturating the mixtures.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

TranslucentID: Detecting Individuals with High Confidence in Saturated DNA SNP Mixtures

Ricke

Watkins

Fremont-Smith

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…A paper by Voskoboinik and Darvasi [8] presented a frame work that requires typing between 1000 and 3000 SNP panels, each with relatively low minor allele frequency, the idea being that an individual is expected to carry dozens of rare alleles, and this set of alleles will be carried in the complex mixture provided an individual with the alleles is a contributor to the mixture.…”

Section: Introductionmentioning

confidence: 99%

“…Voskoboinik and Darvasi [8] have tried to look at a mixture of up to ten contributors, different numbers of independent SNPs and varying minor allele frequencies. As said from previous chapters I shall only be considering a twoperson mixture, and this will not change.…”

Section: Introductionmentioning

confidence: 99%

Identification of Individuals in a DNA Mixture Using SNP Markers

Ibrahim¹

2017

IJGG

View full text Add to dashboard Cite

Abstract:This article focuses mainly on DNA mixture from two contributors, a victim and an unknown culprit. There are two areas I believe will be of interest to forensic scientists, police and a Jury. These areas are identification of an individual in a DNA mixture and familial DNA database searching of a culprit through a relative. In this article, I looked at identification of individuals in a mixture using Single Nucleotide Polymorphisms (SNPs) markers. SNPs are starting to be used for forensic identification; I employed them as they produce incredible results for identification in a two-person mixture. The conservative method I employed here is the random man not excluded probability -P (RMNE) approach, an inclusion probability method generally considered as a frequentist approach. It was found that an optimum allele frequency of 0.2 is required to produce almost certain identification with much distortion in identifying an individual even when inbreeding is up to 50% in a population. Another interesting thing is that relatives of a suspect whom are actual contributors to the DNA mixture can also be identified. In a case where there are relatives in the mixture it was found that twice the number of SNP panels is required to identify an individual than in a case where no relative is involved. And lastly, typing more SNP panels helps to improve identification and therefore produce forensically useful results.

show abstract

“…This is of obvious benefit in addressing sexual offences; however, such offences are also heavily associated with mixed DNA profiles and mixed body fluids. The interpretation of mixed DNA profiles are a particularly complex area in case work [19][20][21][22][23][24], and it would currently be unreasonable to assume that one contributor of the DNA result came from one particular body fluid over the other contributor.…”

Section: Introductionmentioning

confidence: 99%