Forensic genetic informativeness of an SNP panel consisting of 19 multi-allelic SNPs

Gao, Zehua; Chen, Xiaogang; Zhao, Yuancun; Zhao, Xiaohong; Zhang, Shu; Yang, Yiwen; Zhang, Ji

doi:10.1016/j.fsigen.2018.01.006

Cited by 21 publications

(14 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many human identification panels have been developed based on tri-allelic SNPs [17] as tri-allelic SNPs have more discriminatory power [18]. Recently, 8 tri-allelic SNPs were introduced in panel for biogeographical ancestry identification among Chinese Han population [19]. Hence, we further suggest in future introducing informative tri-allelic SNPs studies may bring even better and precise determination of breed purity.…”

Section: Resultsmentioning

confidence: 98%

Identification of breed-specific SNP panel in nine different cattle genomes.

Kumar¹,

Panigrahi²,

Chhotaray³

et al. 2019

biomedicalresearch

View full text Add to dashboard Cite

Single nucleotide polymorphism (SNP) high-density chips are now serving as important bioinformatics tools for improvement and development of various livestock species. Major constraint being the high cost of protocol which is not feasible at the population level. Hence, in the present study, we have tried to reduce the SNP panel to a fewer number of informative markers which will be very much cost-effective. The 50K Illumina BeadChip genotypic data obtained from online Dryad repository for six indigenous cattle breeds, namely Tharparkar, Hariana, Red Sindhi, Sahiwal, Gir and Kankrej were merged with three exotic breeds mainly used in Indian condition, i.e., Holstein-Friesian, Jersey and Brown Swiss. Various quality parameters (MAF-0.36, hwe-0.001, geno-0.95) and statistical operations (F ST , LD values) were applied by different bioinformatics tools. Later, best possible SNPs with an average F ST value of >0.8 were analysed using STRUCTURE 2.3.4 software and we have found perfect clustering among the nine breeds comprising a total of 536 SNPs referring to 158 individuals from nine breeds. Later, breedspecific SNPs were filtered from the set of 536 SNPs using Venny 2.1.0 software.

show abstract

Section: Resultsmentioning

confidence: 98%

Identification of breed-specific SNP panel in nine different cattle genomes.

Kumar¹,

Panigrahi²,

Chhotaray³

et al. 2019

biomedicalresearch

View full text Add to dashboard Cite

show abstract

“…The People's Republic of China is successfully developing key domestic technologies for the STR-PCR amplification kit, mitochondrial DNA sequencing equipment, consumables and reagents used to create a DNA profile data bank (Gao 2018;Mo, 2018; The New Resplendence).…”

Section: Discussionmentioning

confidence: 99%

Genomic Registration Issues In Eurasia And Ways Of Their Solution

Volchetskaya¹,

Волчецкая²,

Макаренко³

et al. 2018

J. Sib. Fed. Univ., Humanit. soc. sci.

View full text Add to dashboard Cite

The article discusses the condition and perspective of DNA identification (genomic registration) in Russia and Eurasia. The authors outline the main issues in the development of forensic registration, such as: lack of introduction of modern technologies, low level of interaction between the institutions, inadequacy of statutory regulation of genomic registration, dependency on foreign supplies. Gaps in the state regulation of genomic registration are noted, issues of legal regulation and law enforcement practice related to the protection of genomic information are highlighted. Due to the increased migration process the DNA identification is an extremely relevant issue for Russia and Eurasia. The aim of the article is to study the theoretical aspects of the legislative policy aimed at DNA identification of the population. It is also aimed at detection and prevention of extremist crimes and at the study of registration practices. Extended analysis revealed that the existing laws and regulations in Russia are not fully effective, it is proposed to introduce additional measures to improve the legal support for genomic registration of the population of Russia and the countries of Eurasia. The possibilities of DNA identification are shown, the prospects of its improvement are analyzed.

show abstract

“…The public SNP database (https://www.ncbi.nlm.nih.gov/ snp) and previous reports offer an opportunity for selecting ideal genetic markers to address various forensic applications. Herein, we selected multi-allelic SNPs from db-SNP database (build 152) and previously published studies (including the relevant supplementary data) [9,10,[12][13][14] by means of two-step screening. First of all, multi-allelic SNPs were filtered with variation classes: SNP; validation status: by 1000 Genomes; minor allele frequency (MAF) > 0.01 and variation alleles:…”

Section: Screening For Appropriate Multi-allelic Snpsmentioning

confidence: 99%

“…The traditional forensic SNP genotyping method often relies on the single base extension reaction, also known as SNaPshot technique, whose products were subsequently detected using capillary electrophoresis [12][13][14]. Nevertheless, distinguishing different SNPs by designing non-overlapping, different length primers or using different fluorescent dyes, as well as the limitation of inherent window space on capillary electrophoresis instrument enables it only to detect minor amounts of SNP loci for a single sample in one reaction system [15,16].…”

Section: Introductionmentioning

confidence: 99%

A set of novel multi‐allelic SNPs for forensic application developed through massively parallel sequencing and its examples of population genetic studies

et al. 2020

View full text Add to dashboard Cite

Massively parallel sequencing (MPS) technology allows to simultaneously type multitudinous molecular genetic markers for many samples in one run with the feature of high detection resolution, and thereby arouses the increasing attention from forensic science. Herein, multiple allelic single nucleotide polymorphisms (multi‐allelic SNPs) were screened for personal identification and parentage testing, and then were genotyped using MPS platform. Unrelated individuals of Chinese Mongolian and Kazakh groups were investigated to further estimate forensic effectiveness and applicability of these multi‐allelic SNPs. The results of sequencing efficiency estimations and forensic genetic statistical parameters demonstrated that this MPS panel of multi‐allelic SNPs was expected to be work for forensic applications. Subsequently, the exploration of population genetic variation patterns among the two investigated groups and other 26 reference populations revealed that these Chinese Mongolian and Kazakh groups had the similar population genetic patterns with the populations from East Asian, but European ancestral composition in the Kazakh group was higher than that in the Mongolian group. Currently, the present results were the preliminary research to scrutinize genetic information of these two ethnic minority groups employing multi‐allelic SNPs.

show abstract

Forensic genetic informativeness of an SNP panel consisting of 19 multi-allelic SNPs

Cited by 21 publications

References 29 publications

Identification of breed-specific SNP panel in nine different cattle genomes.

Identification of breed-specific SNP panel in nine different cattle genomes.

Genomic Registration Issues In Eurasia And Ways Of Their Solution

A set of novel multi‐allelic SNPs for forensic application developed through massively parallel sequencing and its examples of population genetic studies

Contact Info

Product

Resources

About