Forensic evaluation and population genetic study of 30 insertion/deletion polymorphisms in a Chinese Yi group

Abstract

Insertion/deletion polymorphisms have become a research hot spot in forensic science due to their tremendous potential in recent years. In the present study, we investigated 30 indel loci in a Chinese Yi ethnic group. The allele frequencies of the short allele of the 30 indel loci were in the range of 0.1025-0.9221. The power of discrimination values were observed ranging from to 0.2630 (HLD111 locus) to 0.6607 (HLD70 locus) and probability of exclusion values ranged from 0.0189 (HLD111 locus) to 0.2343 (HLD56 locus). The combined power of discrimination and power of exclusion for 30 loci in the studied Yi group were 0.99999999995713 and 0.97746, respectively, which showed tremendous potential for forensic personal identification in the Yi group. Moreover, the DA distances, phylogenetic tree, principal component analysis, and cluster analysis showed the Yi group had close genetic relationships with the Tibetan, South Korean, Chinese Han, and She groups.

Genetic variation and forensic efficiency of autosomal insertion/deletion polymorphisms in Chinese Bai ethnic group: phylogenetic analysis to other populations

“…The LD analysis indicated no significant LD ( r 2 < 0.8) among the 30 loci. We observed the LD analysis of studied Bai group and Chinese Yi group previously reported [27], those two studies obviously verified no linkage among 30 Indel loci, therefore, those loci could be used as independent markers for forensic and population genetic analysis.…”

A 30-InDel Assay for Genetic Variation and Population Structure Analysis of Chinese Tujia Group

“…D A distances between the 16 groups with each other based on allelic frequencies of the 30 InDel loci were shown in Table 2. Short genetic distances were found between the Tujia group and Shanghai Han17, Guangdong Han18, South Korean23, Beijing Han9, Xibe10, She17, Tibetan9, and Yi16 groups; and further distances were observed between the Tujia group and Chinese Kazak9, Uigur9 groups; whereas the larger distances were estimated with Uruguayan24, Dane25, Central Spanish26, Basque26, and Hungarian populations27. Pairwise populations had small genetic distances, which indicated that they had close genetic relationships or shared a recent common ancestor.…”

“…In recent years, a novel genetic marker: insertion and deletion polymorphisms (InDels) dispersing through the human genome showed some advantages, such as short amplicon size, low mutation rate, and practicability of being genotyped in the present forensic DNA lab platforms678, which were useful for forensic DNA applications (Supplementary method for STR applications), population genetics9101112, and biogeographic ancestry analysis131415. Population genetic and forensic validation studies have been performed using the Qiagen Investigator DIPplex ® reagent including 30 autosomal InDel loci plus amelogenin locus, and population data of Chinese Han, Tibetan, Uigur, Kazak, She, Xibe and Yi populations have been reported in previous studies910161718. In the present study, we firstly reported the population genetic data of 30 InDels in Chinese Tujia ethnic group, evaluated their usefulness in the field of forensic sciences, analyzed the interpopulation differentiations, and retraced the genetic background of the Tujia group by the population structure construction, principal component analysis, phylogenetic tree and some other analyses.…”

Genetic affinity between Ningxia Hui and eastern Asian populations revealed by a set of InDel loci

“…To understand the genetic background of the Ningxia Hui population, we compiled obtained data with those from other 21 populations (three Han populations [22][23][24], Yi [25], Xibe [20], South Korean [26], She [23], Tibetan [24], Kazak [24], Uigur [24], five European populations [18,[27][28][29] and six Mexican populations [30]). Pairwise F ST s between these populations were calculated to show their genetic distances (electronic supplementary material, table S1).…”