Forensic DNA typing from teeth using demineralized root tips

Corrêa, Heitor Simões Dutra; Pedro, Fábio Luís Miranda; Volpato, Luiz Evaristo Ricci; Pereira, Thiago Machado; Filho, Gilberto Siebert; Borges, Álvaro Henrique

doi:10.1016/j.forsciint.2017.10.003

Cited by 14 publications

(9 citation statements)

References 46 publications

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“…However, with a few exceptions, teeth are collectively the least successful cranial element from which to sample DNA for STR testing. This runs contrary to other studies indicating that teeth should be preferentially sampled from skeletonized remains . It should be noted that these studies did not use the same portion of the tooth for DNA extraction, nor samples of comparative age.…”

Section: Discussionmentioning

confidence: 62%

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Getting Ahead: Extraction of DNA from Skeletonized Cranial Material and Teeth

Edson

2019

Journal of Forensic Sciences

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Between 1990 and 2018, the Defense POW/MIA Accounting Agency submitted 2177 cranial elements and 1565 teeth to the Armed Forces Medical Examiner System—Armed Forces DNA Identification Laboratory for DNA testing. In an effort to identify missing United States service members, materials were recovered from wartime losses inclusive of World War II, the Korean War, and Southeast Asia. Using four different DNA extraction protocols, DNA testing was performed using mitochondrial DNA Sanger sequencing, modified AmpFlSTR® Yfiler™, AmpFlSTR® MiniFiler™, PowerPlex® Fusion, or Next Generation Sequencing. This paper aims to provide optimal strategies for the DNA testing of skeletonized cranial materials. Cranial elements produced the most consistent results in Sanger sequencing using an organic purification; however, teeth were most successful for the same platform with an inorganic purification. The inverse is true for STR testing of cranial bones. Of the cranial elements, the temporal provided the most consistent results.

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Section: Discussionmentioning

confidence: 62%

“… crushed the entire root of the tooth; Corrêa et al. used the bottom 5 mm of the tooth roots; and Smith et al. and this study drilled the dentine out of the interior of the tooth.…”

Section: Discussionmentioning

confidence: 98%

Getting Ahead: Extraction of DNA from Skeletonized Cranial Material and Teeth

Edson

2019

Journal of Forensic Sciences

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“…The field of forensic odontology involves personal identification of unknown corpses using oral characteristics, including dental treatment marks. It also involves a wide range of tasks such as sex and age estimation using skeletal remains and DNA typing, as well as estimation of time since death [18][19][20][21][22][23][24][25] . Most subjects are appraised using cranial bones, including the calvarium.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Morphological Structures of the Human Calvarium and Turtle Shell

Ishikawa

Hirayama

Miake

et al. 2019

J. Hard Tissue Biology.

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In the field of forensic odontology, not only personal identification using oral conditions, including dental treatment marks and DNA typing, but also species identification, age or sex estimation using cranial or partial bones, and time after death may also be applicable as estimation methods. Among these many tasks, one of the most difficult is species identification of fragmented calvarium. This is because the calvarium has poor morphological features, except that it is a flat bone, and few reports have described how to differentiate it. In this study, a simple identification method for turtle shell (carapace and plastron) whose morphological characteristics closely resembled those of the calvarium was applied. As a result, in an enlarged image obtained using a stereoscopic microscope, the characteristics of each suture pattern could be confirmed. In the decalcified and non-decalcified bone specimens, the difference in the inner/outer laminar structure and the specific structural difference of the cancellous bone-equivalent part were confirmed. Furthermore, most of the features could be obtained in the destructive inspection when it was examined using micro CT imaging to determine whether discrimination by nondestructive inspection was possible or not. No significant difference in ingredients was found using the Electron Probe X-ray Micro Analyzer. The calvarium was more calcified than the carapace but less calcified than the plastron. From these results, we suggest that micro CT imaging is effective for discriminating between the calvarium and turtle shell in a short time.

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“…Incubation takes at least 5 h to overnight in 0.5 M EDTA at 56 o C to completely demineralize the bone powder. Following demineralization, DNA lysates are then purified using organic solvents [ 3 , 4 , 10 , 11 ], silica columns [ 3 , [5] , [6] , [7] , 12 , 13 ], magnetic resins [ 9 , [14] , [15] , [16] ] or a combination of methods [ 17 ]. To increase throughput and reduce amount of sample handling, magnetic resin-based methods has been applied to automated workflows beneficial for mass fatality investigations [ 9 , [15] , [16] , [17] ].…”

Section: Introductionmentioning

confidence: 99%