Forensic characterization and genetic evaluation in the Central Indian population using 27 Y-STRs

Kaitholia, Kamlesh; Dash, Hirak Ranjan; Shrivastava, Pankaj; Kumawat, R. K.; Dixit, Shivani; Chaubey, Gyaneshwer

doi:10.1007/s00414-020-02486-8

Cited by 3 publications

(1 citation statement)

References 5 publications

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“…So far, only a limited number of genetic studies on Y-STRs among the Central Indian population have been reported. Hence, there is a need to conduct more studies on this population [11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Polymorphisms in the Rewa Population of Central India Using Y-Chromosomal STR Markers

Mishra¹,

Gondhali²,

Mishra³

et al. 2021

Sovrem Tehnol Med

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Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Polymorphisms in the Rewa Population of Central India Using Y-Chromosomal STR Markers

Mishra¹,

Gondhali²,

Mishra³

et al. 2021

Sovrem Tehnol Med

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Utilizing Massively Parallel Sequencing (MPS) of Human Leukocyte Antigen (HLA) Gene Polymorphism to Assess Relatedness in Deficiency Parentage Testing

Kouniaki,

Fotopoulos,

Tarassi

et al. 2024

Genes

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In the realm of DNA testing with legal implications, the reliability and precision of genetic markers play a pivotal role in confirming or negating paternity claims. This study aimed to assess the potential utility of human leukocyte antigen (HLA) gene polymorphism through massively parallel sequencing (MPS) technology as robust forensic markers for parentage testing involving genetic deficiencies. It sought to redefine the significance of HLA genes in this context. Data on autosomal short tandem repeat (aSTR) mutational events across 18 paternity cases involving 16 commonly employed microsatellite loci were presented. In instances where traditional aSTR analysis failed to establish statistical certainty, kinship determination was pursued via HLA genotyping, encompassing the amplification of 17 linked HLA loci. Within the framework of this investigation, phase-resolved genotypes for HLA genes were meticulously generated, resulting in the definition of 34 inherited HLA haplotypes. An impressive total of 274 unique HLA alleles, which were classified at either the field 3 or 4 level, were identified, including the discovery of four novel HLA alleles. Likelihood ratio (LR) values, which indicated the likelihood of the observed data under a true biological relationship versus no relationship, were subsequently calculated. The analysis of the LR values demonstrated that the HLA genes significantly enhanced kinship determination compared with the aSTR analysis. Combining LR values from aSTR markers and HLA loci yielded conclusive outcomes in duo paternity cases, showcasing the potential of HLA genes and MPS technology for deeper insights and diversity in genetic testing. Comprehensive reference databases and high-resolution HLA typing across diverse populations are essential. Reintegrating HLA alleles into forensic identification complements existing markers, creating a potent method for future forensic analysis.

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Classical and Non-Classical HLA Alleles as Supplementary Markers in Indirect Kinship Parentage Testing

Kouniaki

Tsirogianni

2022

Immuno

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A civil paternity investigation involving the parents of the deceased alleged father in order to establish a family relationship is presented. On the basis of the 23 autosomal short tandem repeat (aSTR) genotyping results, conclusive proof of paternity was not achieved, as the probability of paternity (W) was calculated to 0.99988. Additional genetic data of 17 classical and non-classical human leukocyte alleles (HLA) typing by next-generation sequencing (NGS) at a high-resolution level supported the hypothesis of grandpaternity over the hypothesis of coincidental paternal obligate allele (POA) sharing (total WaSTR&HLA = 0.9999998). The present study demonstrates the utility of 17 HLA genetic markers-typing in the solution of deficiency cases of disputed parentage.

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Forensic characterization and genetic evaluation in the Central Indian population using 27 Y-STRs

Cited by 3 publications

References 5 publications

Assessment of Genetic Polymorphisms in the Rewa Population of Central India Using Y-Chromosomal STR Markers

Assessment of Genetic Polymorphisms in the Rewa Population of Central India Using Y-Chromosomal STR Markers

Utilizing Massively Parallel Sequencing (MPS) of Human Leukocyte Antigen (HLA) Gene Polymorphism to Assess Relatedness in Deficiency Parentage Testing

Classical and Non-Classical HLA Alleles as Supplementary Markers in Indirect Kinship Parentage Testing

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