Forensic body fluid identification and differentiation by Raman spectroscopy

Muro, Claire K.; Doty, Kyle C.; Fernandes, Luciana de Souza; Lednev, Igor K.

doi:10.1016/j.forc.2016.06.003

Cited by 96 publications

(69 citation statements)

References 50 publications

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“…Near-infrared RS was demonstrated to be an excellent sensitive tool in forensic science and medicine [232,233]. Near-infrared Raman spectra that were recorded from small volumes of saliva, blood and blood serum, urine, semen, sweat and vaginal fluid can be discriminated and identified using advanced mathematical approaches.…”

Section: Rs In Biomedicine and Diagnosticsmentioning

confidence: 99%

Raman Scattering: From Structural Biology to Medical Applications

et al. 2020

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This is a review of relevant Raman spectroscopy (RS) techniques and their use in structural biology, biophysics, cells, and tissues imaging towards development of various medical diagnostic tools, drug design, and other medical applications. Classical and contemporary structural studies of different water-soluble and membrane proteins, DNA, RNA, and their interactions and behavior in different systems were analyzed in terms of applicability of RS techniques and their complementarity to other corresponding methods. We show that RS is a powerful method that links the fundamental structural biology and its medical applications in cancer, cardiovascular, neurodegenerative, atherosclerotic, and other diseases. In particular, the key roles of RS in modern technologies of structure-based drug design are the detection and imaging of membrane protein microcrystals with the help of coherent anti-Stokes Raman scattering (CARS), which would help to further the development of protein structural crystallography and would result in a number of novel high-resolution structures of membrane proteins—drug targets; and, structural studies of photoactive membrane proteins (rhodopsins, photoreceptors, etc.) for the development of new optogenetic tools. Physical background and biomedical applications of spontaneous, stimulated, resonant, and surface- and tip-enhanced RS are also discussed. All of these techniques have been extensively developed during recent several decades. A number of interesting applications of CARS, resonant, and surface-enhanced Raman spectroscopy methods are also discussed.

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Section: Rs In Biomedicine and Diagnosticsmentioning

confidence: 99%

Raman Scattering: From Structural Biology to Medical Applications

et al. 2020

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“…The initial investigation of saliva for forensic purposes using Raman spectroscopy was reported by Virkler and Lednev , including the differentiation of oral fluid from other body fluids including blood, semen, vaginal fluid and sweat. Advanced statistical analysis allowed for the identification of all major body fluids with high confidence . The identification of dry oral fluid as well as other dry body fluids is complicated due to their heterogeneous nature.…”

Section: Introductionmentioning

confidence: 99%

Differentiating smokers and nonsmokers based on Raman spectroscopy of oral fluid and advanced statistics for forensic applications

Al‐Hetlani

Halámková

Amin

et al. 2019

Journal of Biophotonics

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Raman spectroscopy has proven to be a valuable tool for analyzing various types of forensic evidence such as traces of body fluids. In this work, Raman spectroscopy was employed as a nondestructive technique for the analysis of dry traces of oral fluid to differentiate between smoker and nonsmoker donors with the aid of advanced statistical tools. A total of 32 oral fluid samples were collected from donors of differing gender, age and race and were subjected to Raman spectroscopic analysis. A genetic algorithm was used to determine eight spectral regions that contribute the most to the differentiation of smokers and nonsmokers. Thereafter, a classification model was developed based on the artificial neural network that showed 100% accuracy after external validation. The developed approach demonstrates great potential for the differentiation of smokers and nonsmokers based on the analysis of dry traces of oral fluid.

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“…Raman spectroscopy is highly amenable to the forensic community as this methodology is nondestructive, noncontact, and identifications are generally considered confirmatory . Additionally, an automatic classification model that allows for confirmatory identification of all major body fluids when deposited on a noninterfering substrate (aluminum foil) has been developed . Beyond identification, deeper information can be elucidated from Raman spectra of certain fluids, including species of origin, race of an individual, gender, donor age, and time since deposition using chemometrics …”

Section: Introductionmentioning

confidence: 99%

Universal detection of body fluid traces in situ with Raman hyperspectroscopy for forensic purposes: Evaluation of a new detection algorithm (HAMAND) using semen samples

McLaughlin

Fikiet

Ando³

et al. 2019

J Raman Spectroscopy

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Towards closing the technological gaps in forensic science, Raman spectroscopy has been a boon to the field. One area that this technique shows exceptional promise is in body fluid identification and characterization, but substrate interference remains a major impediment to its practical implementation. Here, we present an approach for the universal detection of body fluids regardless of the substrate. This approach, which is based on Raman hyperspectroscopy and multivariate curve resolution, was applied to datasets representing simulated semen evidence. In every instance, the signal of the fluid was extracted and matched to a reference of semen. This approach has immediate application for body fluid detection and allows for a universal, automatic, nondestructive, on‐field method for confirmatory identification of body fluid traces at a crime scene. This approach is applicable where an analyte is either a minor contributor or spatially distributed on a strongly interfering substrate.

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Forensic body fluid identification and differentiation by Raman spectroscopy

Cited by 96 publications

References 50 publications

Raman Scattering: From Structural Biology to Medical Applications

Raman Scattering: From Structural Biology to Medical Applications

Differentiating smokers and nonsmokers based on Raman spectroscopy of oral fluid and advanced statistics for forensic applications

Universal detection of body fluid traces in situ with Raman hyperspectroscopy for forensic purposes: Evaluation of a new detection algorithm (HAMAND) using semen samples

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