Forensic application of VEGF expression to skin wound age determination

Hayashi, Takahito; Ishida, Yuko; Kimura, Akihiko; Takayasu, Tatsunori; Eisenmenger, Wolfgang; Kondo, Toshikazu

doi:10.1007/s00414-004-0468-x

Cited by 84 publications

(67 citation statements)

References 45 publications

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“…Although it is often read that neutrophils are recruited to skin wounds within minutes [17,18], articles providing original information [6,[19][20][21] and detailed reviews [22,23] indicate appreciable neutrophil infiltration onsets only since at least 1 h or more after wounding.…”

Section: Discussionmentioning

confidence: 98%

Early increase in TNF-alpha-containing mast cells in skin lesions

et al. 2005

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We have evaluated the numbers and immunohistochemical positivity for tumor necrosis factor (TNF) alpha of the mast cells in (a) 40 skin samples collected at autopsy from subjects who had survived for a few seconds to 1 h, (b) 10 samples of post-mortem skin lesions and (c) 10 surgical biopsies of healthy skin. Sections were treated with fluoresceinated avidin, to tag mast cell granules, followed by indirect immunohistochemistry for TNF-alpha with polyclonal primary and rhodaminated secondary antibodies. We could confirm a progressive increase in mast cell numbers, which became significant 1 h after trauma. More important, the fraction of mast cells positive for TNF-alpha increased progressively in the same time period and became significantly higher than controls in specimens collected more than 15 min after trauma. Samples from post-mortem lesions had significantly fewer mast cells and fewer TNF-alpha-positive cells than any other group of samples. The results suggest that mast cells are quickly recruited to an injured site in response to trauma and upregulate their TNF-alpha content, which can play an early role in directing tissue response to injury. The forensic pathologist can take advantage from this behavior of mast cells for the evaluation of the timing of early vital lesions.

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Section: Discussionmentioning

confidence: 98%

Early increase in TNF-alpha-containing mast cells in skin lesions

et al. 2005

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“…P-selectin was found to be expressed in the earliest stages of wound formation within a period of 3 minutes to 7 hours (17,18). Similarly, Hayashi et al (19) suggested that VEGF-positive reaction may indicate a wound age of 7-21 days.…”

Section: Discussionmentioning

confidence: 94%

Immunohistochemical determination of wound age in mice

Akbaba¹,

Kara²,

Demi̇r³

et al. 2014

Gaziantep Med J

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Determination of wound age is one of the greatest challenges in autopsy cases. We have little knowledge on this issue despite a plethora of current studies. The purpose of this study was to shed light on wound age determination by studying the expression levels of ubiquitin and Ki-67 through immunohistochemical methods. A total of 45 (Balb/c) 6-8 week-old male mice, weighing 25-30 grams were divided into nine groups including five mice in each group. After general anesthesia, a 1.5 cm full-thickness incision was made on the dorsal skin using a scalpel. At 1, 3, 6, 12, and 24 hours and 5, 7, and 14 days, a total of 42 mice were sacrificed by cervical dislocation. An area of 1 cm surrounding the wound was excised. One mouse died in each of the Groups 6, 7, and 8, leaving 42 mice to complete the study. The mean number of fibroblasts with positive Ki-67 staining at the wound edge was significantly higher in Groups 4, 5 and 6. Groups 5 and 6 showed a statistically significant percentage of Ki-67 positive staining basal cells. Groups 7 and 8 exhibited similar features with the control group. Ubiquitin-positive fibroblast cells in Groups 1 to 4 showed similar features with the control group and the number of ubiquitin-positive fibroblast cells was statistically significant in Groups 5, 6 and 7. As a marker, ubiquitin may be useful in determining whether the wound age is older than 1 day and may be used for wounds 1 to 7 days old. We suggest that the Ki-67 marker may assist in the determination of the age of 1 to 5-day-old wounds by using immunohistochemical methods.

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“…W cytowanym badaniu ekspresja tego białka była istotnie niższa w porówna-niu z grupą kontrolną i grupą przypadków zgonów w przebiegu wstrząsu hipowolemicznego. Kolejnym kierunkiem badawczym, analizowanym zarówno w modelach zwierzęcych [8], jak i na materiale ludzkim [9,10], jest ocena wieku ran na podstawie immuprocess of vasculogenesis which mainly occurs during embryonic development [2,3].…”

Section: Angiogeneza W Medycynie Sądowejunclassified

“…In the cited study, the expression of the protein was significantly lower in relation to the control group and the group of deaths from hypovolaemic shock. Another research direction, which is pursued both in animal models [8] and the human material [9,10], is the assessment of wound age on the basis of the immunohistochemical expression of factors involved in the formation of new blood nohistochemicznej ekspresji czynników związanych z tworzeniem nowych naczyń krwionośnych i limfatycznych oraz hipoksją tkankową, takich jak CD31, czynnik von Willebranda (vWF), VEGF, podoplanina, ORP150 i izoformy syntazy tlenku azotu (NOS) [8][9][10][11]. Na uwagę zasługują także próby wykorzystania oceny pourazowej ekspresji cyklooksygenaz COX-1 i COX-2 w mózgu [12].…”

Section: Angiogenesis In Forensic Medicineunclassified

“…: angiostatyna, endostatyna, trombospondyna 1, czynnik płyt-kowy 4 (PF 4) i interferon α (IFN-α). and lymphatic vessels, and tissue hypoxia -including CD31, von Willebrand factor (vWF), VEGF, podoplanin, ORP150 and NOS (nitric oxide synthase) isoforms (NOS) [8][9][10][11]. Also noteworthy are the attempts to use the assessment of post-traumatic expression of cyclooxygenases COX-1 and COX-2 in the brain [12].…”

Section: Mechanizmy Angiogenezymentioning

confidence: 99%

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