Forelimb development in <i>Amblystoma punctatum</i> following x‐irradiation of either ectoderm or mesoderm

Piatt, Jean; Kusner, David B.

doi:10.1002/jez.1401450308

Cited by 6 publications

(2 citation statements)

References 20 publications

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“…Considered together, results from these studies point toward the existence of a shadow enhancer for Shh in urodele limbs as well. With the loss of a morphological and molecular AER (Purushothaman et al, 2019), the limb development program in urodeles relocated into the mesoderm with a role for the ectoderm diminished (Piatt and Kusner, 1960;Lauthier, 1985).…”

Section: Discussionmentioning

confidence: 99%

Sonic hedgehog is Essential for Proximal-Distal Outgrowth of the Limb Bud in Salamanders

Purushothaman

Aviña

Seifert

2022

Front. Cell Dev. Biol.

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The developing forelimb has been a foundational model to understand how specified progenitor cells integrate genetic information to produce the tetrapod limb bauplan. Although the reigning hypothesis is that all tetrapods develop limbs in a similar manner, recent work suggests that urodeles have evolved a derived mode of limb dvelopment. Here, we demonstrate through pharmacological and genetic inactivation of Sonic hedgehog (Shh) signaling in axolotls that Shh directs expansion and survival of limb progenitor cells in addition to patterning the limb across the proximodistal and antero-posterior axis. In contrast to inactivation of Shh in mouse or chick embryos where a humerus, radius, and single digit develop, Shh crispant axolotls completely lack forelimbs. In rescuing limb development by implanting SHH-N protein beads into the nascent limb field of Shh crispants, we show that the limb field is specified in the absence of Shh and that hedgehog pathway activation is required to initiate proximodistal outgrowth. When our results are examined alongside other derived aspects of salamander limb development and placed in a phylogenetic context, a new hypothesis emerges whereby the ability for cells at an amputation plane to activate morphogenesis and regenerate a limb may have evolved uniquely in urodeles.

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Section: Discussionmentioning

confidence: 99%

Sonic hedgehog is Essential for Proximal-Distal Outgrowth of the Limb Bud in Salamanders

Purushothaman

Aviña

Seifert

2022

Front. Cell Dev. Biol.

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“…Considered together, the results from these studies point towards the existence of a shadow enhancer for Shh in urodeles limbs similar to fishes. With the loss of a morphological and molecular AER (3), the limb development program in urodeles relocated into the mesoderm with a role for the ectoderm diminished (75,76). The derived nature of the urodele limb program raises the intriguing hypothesis that the ability to regenerate an adult limb evolved in this lineage as opposed to limb regeneration being ancestral for tetrapods only to be lost in all the other major extant lineages (Figure 6).…”

Section: Discussionmentioning

confidence: 99%

Sonic hedgehog is essential for proximal-distal outgrowth of the limb bud in salamanders

Seifert

Purushothaman

Aviña

2021

Preprint

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The developing forelimb has been a foundational model to understand how specified progenitor cells integrate genetic information to produce the tetrapod limb bauplan (1, 2). Although the reigning hypothesis is that all tetrapods develop limbs in a similar manner, recent work suggests that urodeles have evolved a derived mode of limb development (3-5). Here we demonstrate through pharmacological and genetic inactivation of Sonic hedgehog (Shh) signaling in axolotls that Shh directs expansion and survival of limb progenitor cells in addition to patterning the limb across the proximodistal and antero-posterior axis. In contrast to inactivation of Shh in mouse or chick embryos where a humerus, radius and single digit develop (6-9), Shh crispant axolotls completely lack forelimbs. In rescuing limb development by implanting SHH-N protein beads into the nascent limb field of Shh-crispants, we show that the limb field is specified in the absence of Shh and that hedgehog pathway activation is required to initiate proximodistal outgrowth. When the derived nature of salamander limb development is placed in a phylogenetic context, it generates a new hypothesis where the ability to regenerate an entire tetrapod limb may have evolved uniquely among urodeles.

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Reactions of the x‐irradiated embryonic eye in a normal field, and vice versa

Rugh

Dyke

1962

J. Exp. Zool.

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Forelimb development in Amblystoma punctatum following x‐irradiation of either ectoderm or mesoderm

Cited by 6 publications

References 20 publications

Sonic hedgehog is Essential for Proximal-Distal Outgrowth of the Limb Bud in Salamanders

Sonic hedgehog is Essential for Proximal-Distal Outgrowth of the Limb Bud in Salamanders

Sonic hedgehog is essential for proximal-distal outgrowth of the limb bud in salamanders

Reactions of the x‐irradiated embryonic eye in a normal field, and vice versa

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