Forces of Change: Optical Tweezers in Membrane Remodeling Studies

Cheppali, Sudheer Kumar; Dharan, Raviv; Sorkin, Raya

doi:10.1007/s00232-022-00241-1

Cited by 5 publications

(2 citation statements)

References 107 publications

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“…An attractive approach for quantitative studies of protein–protein or protein–membrane interactions is by using optical tweezers. − Several experimental schemes have been used; one approach is using microscopic beads coated with synthetic membranes, where ectodomains of proteins of interest are tethered to the microspheres using linkers. , Another approach is using soluble ectodomains with membrane-coated beads, ,− or binding proteins to synthetic membranes via His-tag on the protein interacting with Ni-NTA headgroup-modified lipids . All of these studies involved microspheres coated with synthetic membranes and truncated rather than full-length proteins.…”

Section: Introductionmentioning

confidence: 99%

Supported Natural Membranes on Microspheres for Protein–Protein Interaction Studies

Cheppali

Dharan

Katzenelson

et al. 2022

ACS Appl. Mater. Interfaces

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Multiple biological and pathological processes, such as signaling, cell−cell communication, and infection by various viruses, occur at the plasma membrane. The eukaryotic plasma membrane is made up of thousands of different lipids, membrane proteins, and glycolipids, and its composition is dynamic and constantly changing. Due to the central importance of membranes on the one hand and their complexity on the other, membrane model systems are instrumental for interrogating membrane-related biological processes. Here, we develop a new tool for protein−membrane interaction studies. Our method is based on natural membranes obtained from extracellular vesicles. We form membrane bilayers supported on polystyrene microspheres that can be trapped and manipulated using optical tweezers. This method allows working with membrane proteins of interest within a background of native membrane components where their correct orientation is preserved. We demonstrate our method's applicability by successfully measuring the interaction forces between the Spike protein of SARS-CoV-2 and its human receptor, ACE2. We further show that these interactions are blocked by the addition of an antibody against the receptor binding domain of the Spike protein. Our approach is versatile and broadly applicable for various membrane biology and biophysics questions.

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Section: Introductionmentioning

confidence: 99%

Supported Natural Membranes on Microspheres for Protein–Protein Interaction Studies

Cheppali

Dharan

Katzenelson

et al. 2022

ACS Appl. Mater. Interfaces

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“…Optical tweezers have become a powerful tool for application in different fields of research, mainly in the manipulation of biological system [1,2,3,4], colloidal systems [5], in nanotechnology for trapping of nano-structures [6,7], in optical guiding and trapping of atoms [8,9] as well as the study of mechanical properties of polymers and biopolymers [10]. Initially, Ashkin et al [11] were able to capture in threedimensional dielectric particles using a single beam well focused by a high numerical aperture lens.…”

Section: Introductionmentioning

confidence: 99%

Experimental optical trapping with frozen waves

et al. 2020

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In this work, we report, to the best of our knowledge, the first optical trapping experimental demonstration of micro-particles with Frozen Waves. Frozen Waves are an efficient method to model longitudinally the intensity of non-diffracting beams obtained by superposing co-propagating Bessel beams with the same frequency and order. Based on this, we investigate the optical force distribution acting on micro-particles of two types of Frozen Waves. The experimental setup of a holographic optical tweezers using spatial light modulators has been assembled and optimized. The results show that it is possible to obtain greater stability for optical trapping using Frozen waves. The significant enhancement in trapping geometry from this approach shows promising applications for optical tweezers, micro-manipulations over a broad range.

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Preface to Special Issue on Protein-Mediated Membrane Remodeling

Chakraborty

Sengupta

2022

J Membrane Biol

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Forces of Change: Optical Tweezers in Membrane Remodeling Studies

Cited by 5 publications

References 107 publications

Supported Natural Membranes on Microspheres for Protein–Protein Interaction Studies

Supported Natural Membranes on Microspheres for Protein–Protein Interaction Studies

Experimental optical trapping with frozen waves

Preface to Special Issue on Protein-Mediated Membrane Remodeling

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