Forced expression of Sox2 or Nanog in human bone marrow derived mesenchymal stem cells maintains their expansion and differentiation capabilities

Go, Masahiro J.; Takenaka, Chiemi; Ohgushi, Hajime

doi:10.1016/j.yexcr.2007.11.021

Cited by 121 publications

(115 citation statements)

References 30 publications

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“…These phenomena were also observed as a result of Sox2 overexpression in which type Iα1, osteonectin, and osteocalcin expressions were upregulated by 3.83-, 1.97-, and 5.93-fold, respectively ( Figure 7B). The Sox2-overexpressed cells showed higher osteogenesis potential in the presence of bFGF (Go et al, 2008). These observations indicated that Oct4 or Sox2 overexpression also improved the osteogenesis of BMSCs.…”

Section: Osteogenesis Of Oct4 or Sox2-overexpressed Bmscsmentioning

confidence: 71%

“…During passage, the Oct4-and Sox2-overexpressed cells were relatively small and grew very well, whereas the control cells became elongated in shape ( Figure 5B). Moreover, Sox2-overexpressed cells show higher proliferation potential in the presence of bFGF (Go et al, 2008). These observations suggested that Oct4 or Sox2 overexpression not only promoted the proliferation of BMSCs, but also maintained the morphology of the cells.…”

Section: Growth Properties Of Oct4 or Sox2 Overexpression On Bmscsmentioning

confidence: 80%

“…In addition, Sox2-knockout cells could not form colonies, and their population growth was arrested with a senescent phenotype (Basu-Roy et al, 2010). In another study, most Sox2-overexpressed BMSCs were small and showed high proliferative and osteogenic capabilities from human BMSCs (Go et al, 2008). Knockdowns of Sox2 and Msx2 using RNA interference, showed a significant decrease in colony-forming ability.…”

Section: Discussionmentioning

confidence: 99%

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Oct4 and Sox2 overexpression improves the proliferation and differentiation of bone mesenchymal stem cells in Xiaomeishan porcine

Fan¹,

Gu²,

Zhang³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Mesenchymal stem cells derived from bone marrow (BMSCs) are a population of self-renewing multipotent cells that are capable of differentiating into various cellular lineages, and are widely employed in tissue engineering and cell therapy. Recently, clinical research involving BMSCs has become increasingly popular. In order to conduct appropriate research, it is first necessary to amplify large amounts of functional BMSCs in vitro. However, after several passages of expanding in vitro, the proliferation and differentiation potential of BMSCs gradually decline. To determine whether overexpression of Oct4 or Sox2 might prevent this decline, we transfected Oct4 or Sox2, which are essential for the pluripotency and self-renewal of embryonic stem cells, into BMSCs of Xiaomeishan porcine by a lentivirus. The results showed that overexpression of Sox2 or Oct4 BMSCs in culture media containing a basic fibroblast growth factor resulted in higher proliferation and differentiation compared to controls, suggesting that genetic modification of stemness-related genes is an efficient way to maintain the proliferation and differentiation potential of BMSCs.

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Section: Osteogenesis Of Oct4 or Sox2-overexpressed Bmscsmentioning

confidence: 71%

Section: Growth Properties Of Oct4 or Sox2 Overexpression On Bmscsmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Oct4 and Sox2 overexpression improves the proliferation and differentiation of bone mesenchymal stem cells in Xiaomeishan porcine

Fan¹,

Gu²,

Zhang³

et al. 2013

Genet. Mol. Res.

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show abstract

“…Recently, it has been reported that MSCs can maintain their expansion and differentiation capabilities by the forced expression of Sox2 or Nanog transcription factors [13]. The Sox2-expressing cells in the presence of basic fibroblast growth factor show higher proliferation and differentiation potentials and the cells are smaller than control cells.…”

Section: Discussionmentioning

confidence: 99%

Development of an early estimation method for predicting later osteogenic differentiation activity of rat mesenchymal stromal cells from their attachment areas

Cheng

Hirose

Wang

et al. 2012

Science and Technology of Advanced Materials

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Cell morphology has received considerable attention in recent years owing to its possible relationship with cell functions, including proliferation, differentiation, and migration. Recent evidence suggests that extracellular environments can also mediate cell functions, particularly cell adhesion. The aims of this study were to investigate the correlation between osteogenic differentiation activity and the morphology of rat mesenchymal stromal cells (MSCs), and to develop a method of estimating osteogenic differentiation capability of MSCs on biomaterials. We measured the attachment areas of MSCs on substrates with various types of surface after 2 h of seeding, and quantified the amount of osteocalcin secreted from MSCs after 3 weeks of culture under osteogenic differentiation conditions. MSCs with small attachment areas showed a high osteogenic differentiation activity. These findings indicate that cell attachment areas correlate well with the osteogenic differentiation activity of MSCs. They also suggest that the measurement of cell attachment areas is useful for estimating the osteogenic differentiation activity of MSCs and is a practical tool for applications of MSCs in regenerative medicine.

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“…33 For example, bFGF has been shown to promote proliferation but prevent osteogenic 34 or adipogenic 35 pluripotency through distinct pathways. The effect of bFGF on hMSC proliferation was mediated via Erk2 but not Erk1 mitogen-activated kinase.…”

mentioning

confidence: 99%

Derivation of Functional Smooth Muscle Cells from Multipotent Human Hair Follicle Mesenchymal Stem Cells

Liu

Peng

Gopinath

et al. 2010

Tissue Engineering Part A

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We investigated the potential of human hair follicle cells for multilineage differentiation and as a source of functional smooth muscle cells (SMCs). We report that human hair follicle stem cells (HFCs) isolated from individual follicles expressed surface markers that are characteristic of mesenchymal stem cells such as CD44, CD49b, CD73, CD90, and CD105 but lacked hematopoietic markers CD45 and CD34. In addition, HFCs differentiated toward adipocytes, chondrocytes, osteoblasts, or SMCs in the appropriate induction medium. Treatment with basic fibroblast growth factor increased proliferation and prevented myogenic differentiation, suggesting that basic fibroblast growth factor can be used to expand the population of undifferentiated HFCs to the large numbers needed for therapeutic applications. SMCs were isolated from HFCs using tissue-specific promoters and flow cytometry sorting. Cylindrical vascular constructs engineered with HF-SMCs showed remarkable contractility in response to receptor and nonreceptor agonists such KCl, endothelin-1, and the thromboxane mimetic, U46619, as well as superior mechanical properties compared to their counterparts with human vascular SMCs. Our results suggest that HF is a rich source of mesenchymal stem cells with great potential for myogenic differentiation providing functional SMCs for tissue regeneration and cell therapies.

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Forced expression of Sox2 or Nanog in human bone marrow derived mesenchymal stem cells maintains their expansion and differentiation capabilities

Cited by 121 publications

References 30 publications

Oct4 and Sox2 overexpression improves the proliferation and differentiation of bone mesenchymal stem cells in Xiaomeishan porcine

Oct4 and Sox2 overexpression improves the proliferation and differentiation of bone mesenchymal stem cells in Xiaomeishan porcine

Development of an early estimation method for predicting later osteogenic differentiation activity of rat mesenchymal stromal cells from their attachment areas

Derivation of Functional Smooth Muscle Cells from Multipotent Human Hair Follicle Mesenchymal Stem Cells

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