Force-velocity characteristics of isolated myocardium preparations from rats exposed to subchronic intoxication with lead and cadmium acting separately or in combination

Cardiotoxicity in rats following subchronic exposure to cadmium chloride solution and cadmium oxide nanoparticles

“…In both experiments, we modelled moderate subchronic exposure levels and observed statistically significant shifts in a number of rat indices indicating the development of subchronic cadmium poisoning of moderate severity. Toxic effects of cadmium, with a focus on cardiovascular impairments, were described in detail in our previous articles 20,22,23,26,27 . This study is mainly devoted to a qualitative comparison of the effects of CdCl 2 and CdO NPs.…”

“…The ratios of α-and β-myosin heavy chains were determined by gel electrophoresis (SDS-PAGE). The velocity of thin reconstituted filament consisting of actin, troponin, and tropomyosin, sliding over myosin was studied by an in vitro motility assay described previously 21,22 . Reconstituted thin filament moved on a surface coated with rat myosin in a flow cell in the presence of ATP and calcium ions (pCa=4).…”

Functional consequences of lead and mercury exposomes in the heart

“…These experiments are consistent with the negative inotropic effect found after chronic exposure to Pb 2+ by breastfeeding of neonatal rats (Williams et al, 1983). It has been proposed from the experiments in rats that even low levels of Pb 2+ interfere with the contractile proteins of the heart and their contraction cycle (Katsnelson et al, 2020;Silva et al, 2015). More recent reports also found a negative inotropic of the Pb 2+ exposome (Klinova et al, 2020;Protsenko et al, 2018Protsenko et al, , 2019.…”

Analysis of changes in the rat cardiovascular system under the action of lead intoxication and muscular exercise

“…Препараты фиксировали к штокам сервомотора длины и датчика силы, помещали в терморегулируемую ванну (Muscle Research System, Scientific Instruments GmbH, Германия). Регистрацию сокращений изолированных препаратов миокарда в изометрическом и физиологическом режимах проводили, как описано ранее [19,20].…”

“…При свинцовой интоксикации снижение скоростных и рост временных показателей изометрического сокращения, а также снижение максимальной скорости изотонического укорочения коррелирует со снижением скорости скольжения регулируемых тонких филаментов и изменением соотношения α-и β-тяжёлых цепей миозина (ТЦМ) и частично объясняется ростом доли более медленных β-ТЦМ. Данные корреляции были показаны нами ранее в аналогичных экспериментах [20].…”