Foot and Mouth Disease Virus Genome

Carrillo, C.

doi:10.5772/26930

Cited by 6 publications

(3 citation statements)

References 41 publications

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“…Nonstructural proteins (NSPs) L, 2A-C, and 3A-D are required for viral replication and interactions with host cell factors. 5,6 Because highly purified FMDV vaccines lack NSPs, differentiation of infected from vaccinated animals (DIVA) by assays detecting FMDV NSP-specific antibodies can be used in endemic regions with active FMDV vaccination programs. 9,35 NSP-purified vaccines and DIVA assays are crucial to demonstrate FMDV-free status in countries and regions that have, or are interested in, FMDV vaccinationbased control programs.…”

Section: Introductionmentioning

confidence: 99%

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An improved, rapid competitive ELISA using a novel conserved 3B epitope for the detection of serum antibodies to foot-and-mouth disease virus

Chung

Clavijo²,

Bounpheng

et al. 2018

J VET Diagn Invest

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The highly contagious foot-and-mouth disease virus (FMDV) afflicts cloven-hoofed animals, resulting in significant costs because of loss of trade and recovery from disease. We developed a sensitive, specific, and rapid competitive ELISA (cELISA) to detect serum antibodies to FMDV. The cELISA utilized a monoclonal blocking antibody specific for a highly conserved FMDV nonstructural 3B epitope, a recombinant mutant FMDV 3ABC coating protein, and optimized format variables including serum incubation for 90 min at 20-25°C. Samples from 16 animals experimentally infected with one FMDV serotype (A, O, Asia, or SAT-1) demonstrated early detection capacity beginning 7 d post-inoculation. All samples from 55 vesicular stomatitis virus antibody-positive cattle and 44 samples from cloven-hoofed animals affected by non-FMD vesicular diseases were negative in the cELISA, demonstrating 100% analytical specificity. The diagnostic sensitivity was 100% against sera from 128 cattle infected with isolates of all FMDV serotypes, emphasizing serotype-agnostic results. Diagnostic specificities of U.S. cattle ( n = 1135) and swine ( n = 207) sera were 99.4% and 100%, respectively. High repeatability and reproducibility were demonstrated with 3.1% coefficient of variation in percent inhibition data and 100% agreement using 2 kit lots and 400 negative control serum samples, with no difference between bench and biosafety cabinet operation. Negative results from vaccinated, uninfected cattle, pig, and sheep sera confirmed the DIVA (differentiate infected from vaccinated animals) capability. This rapid (<3 h), select agent-free assay with high sensitivity and specificity, DIVA capability, and room temperature processing capability will serve as a useful tool in FMDV surveillance, emergency preparedness, response, and outbreak recovery programs.

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Section: Introductionmentioning

confidence: 99%

“…Nonstructural proteins (NSPs) L, 2A–C, and 3A–D are required for viral replication and interactions with host cell factors. 5,6…”

Section: Introductionmentioning

confidence: 99%

An improved, rapid competitive ELISA using a novel conserved 3B epitope for the detection of serum antibodies to foot-and-mouth disease virus

Chung

Clavijo²,

Bounpheng

et al. 2018

J VET Diagn Invest

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show abstract

“…The etiological agent, the FMD virus (FMDV), belongs to the family Picornaviridae and genus Aphthovirus (3). This virus exists as 7 immunogenically different serotypes, O, A, Asia1, C, SAT1, SAT2, and SAT3, with multiple subtypes within each serotype (4,5). Infection with one serotype does not offer cross-protection against other serotypes (6).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of PCR primers targeting the VP2 region of the foot-and-mouth disease virus for improved serotype detection

Ali¹,

Habib²,

Shah³

2018

Turk J Vet Anim Sci

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Frequently, a sequence from a single strain of a virus is used to design primers for PCR-based virus detection. However, high mutation rates in RNA viruses lead to failure of microorganism detection in clinical samples. Therefore, it is essential to find conserved regions within sequences to design primers for the detection of pathogen genomes in suspected clinical samples. The aim of the study was to find conserved regions of the genome for improved serotype detection. In the present study, primers were designed for serotype detection based on the VP2 coding region of foot-and-mouth disease virus (FMDV) after alignment of full genome (n = 375) sequences. These primer pairs and previously reported primer pairs were then compared using positive samples (n = 91) collected during 2010-2016 from Faisalabad District, Pakistan. The detection rate of newly designed primer pairs O-Wqs-F/Rev-Wqs (96.5%), As-Wqs-F/Rev-Wqs (90.4%), and A-Wqs-F/Rev-Wqs (100%) was better compared with previously reported primer pairs P38/P33 (81%), P74-77/P33 (47.6%), and P87-92/P33 (41.6%) in detecting FMDV serotypes O, Asia1, and A, respectively. The higher detection rates of the newly designed primer pairs appeared to be due to the selection of highly conserved sequences within the serotypes for designing primers. To the best of the authors' knowledge, this is the first study that describes serotype diagnosis of FMDV based on primers targeting the VP2 region. In conclusion, this new method offers an improved approach for the serotyping of FMDV compared to previous methods.

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Adjuvant effect of saponin in an oil-based monovalent (serotype O) foot-and-mouth disease virus vaccine on the antibody response in guinea pigs and cattle

et al. 2021

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Foot and Mouth Disease Virus Genome

Cited by 6 publications

References 41 publications

An improved, rapid competitive ELISA using a novel conserved 3B epitope for the detection of serum antibodies to foot-and-mouth disease virus

An improved, rapid competitive ELISA using a novel conserved 3B epitope for the detection of serum antibodies to foot-and-mouth disease virus

Evaluation of PCR primers targeting the VP2 region of the foot-and-mouth disease virus for improved serotype detection

Adjuvant effect of saponin in an oil-based monovalent (serotype O) foot-and-mouth disease virus vaccine on the antibody response in guinea pigs and cattle

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