Foot-and-mouth disease virus: A first inter-laboratory comparison trial to evaluate virus isolation and RT-PCR detection methods

Ferris, N.P.; King, Donald P.; Reid, Scott M.; Hutchings, G.; Shaw, Andrew; Paton, David; Goris, Nesya; Haas, Bernd; Hoffmann, Bernd; Brocchi, Emiliana; Bugnetti, M.; Dekker, A.; Clercq, Kris De

doi:10.1016/j.vetmic.2006.06.001

Cited by 36 publications

(34 citation statements)

References 24 publications

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“…Primary lamb kidney (LK) cells are also very sensitive to FMDV, and unlike BTY cells, LK cells maintain their sensitivity to FMDV infection after cryopreservation (5). Immortalized cell lines (e.g., baby hamster kidney fibroblasts and porcine kidney epithelial cells), while much easier to maintain, are in many cases less susceptible to specific animal-derived FMDV serotypes (6)(7)(8)(9). There is a need for a cell line that is easily maintained and is highly susceptible to all serotypes and subtypes of FMDV.…”

mentioning

confidence: 99%

A Continuous Bovine Kidney Cell Line Constitutively Expressing Bovine α_Vβ₆Integrin Has Increased Susceptibility to Foot-and-Mouth Disease Virus

LaRocco

Krug

Kramer³

et al. 2013

J Clin Microbiol

133

113

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Foot-and-mouth disease (FMD) is a worldwide problem limiting the trade of animals and their products from affected countries. The rapid isolation, serotyping, and vaccine matching of FMD virus from disease outbreaks is critical for enabling the implementation of effective vaccination programs and to stop the spread of infection during outbreaks. Some primary cells have been shown to be highly susceptible to most strains of FMD virus (FMDV) but are difficult and expensive to prepare and maintain. Since the ␣ V ␤ 6 integrin is a principal receptor for FMDV, we transduced a bovine kidney cell line to stably express both the ␣ V and ␤ 6 bovine integrin subunits. This stable cell line (LFBK-␣ V ␤ 6 ) showed ␤ 6 expression and enhanced susceptibility to FMDV infection for >100 cell passages. LFBK-␣ V ␤ 6 cells were highly sensitive for detecting all serotypes of FMDV from experimentally infected animals, including the porcinophilic FMDV strain O/TAW/97. In comparison to other cell types that are currently used for virus isolation, LFBK-␣ V ␤ 6 cells were more effective at detecting FMDV in clinical samples, supporting their use as a more sensitive tool for virus isolation.F oot-and-mouth disease virus (FMDV) is a severe economic concern for meat-producing nations since the trade of animal products is limited in the countries where the virus is present. The rapid spread of the virus among susceptible animals results in severe morbidity and, in some cases, death, especially in young animals (reviewed in reference 1). Infection or vaccination with one of the seven different serotypes does not confer cross-protection to other serotypes or even to some subtypes of the same serotype. Vaccines for FMDV are widely used to prevent clinical disease, but since vaccines are serotype and subtype specific, the virus(es) causing outbreaks must be isolated and serologically characterized for vaccine matching prior to selecting the appropriate vaccine antigen to be used in vaccine formulations (reviewed in reference 2).Although molecular techniques, such as PCR coupled with genomic sequencing, can be used in samples containing enough virus to rapidly identify the virus serotype and its relationship to other FMDV strains, appropriate vaccine prediction requires virus growth in cell culture to carry out neutralization tests using reference sera. Inefficient recovery of virus from animal samples can delay diagnosis and vaccine selection, hampering the rapid implementation of control measures; thus, virus isolation protocols are designed for maximum sensitivity. Some primary cells, such as bovine thyroid (BTY), are highly susceptible to a wide range of FMDV serotypes (3), but they are difficult and costly to prepare and lose FMDV susceptibility after multiple passages (4). Primary lamb kidney (LK) cells are also very sensitive to FMDV, and unlike BTY cells, LK cells maintain their sensitivity to FMDV infection after cryopreservation (5). Immortalized cell lines (e.g., baby hamster kidney fibroblasts and porcine kidney epithelial cells), ...

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mentioning

confidence: 99%

A Continuous Bovine Kidney Cell Line Constitutively Expressing Bovine α_Vβ₆Integrin Has Increased Susceptibility to Foot-and-Mouth Disease Virus

LaRocco

Krug

Kramer³

et al. 2013

J Clin Microbiol

133

113

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“…An assay is considered valid only to the extent that its results accurately predict, by inference, the true status of the samples being examined (Ferris et al 2006). In fact, any test may not prove presence or absence of infection, but its result can give more accurate probability of its presence or absence (post-test probability).…”

Section: Fig 2 Post-test Probability Of An Individual Being Infectedmentioning

confidence: 99%

Inter-laboratory evaluation of a duplex RT-PCR method using crude extracts for the simultaneous detection of Prune dwarf virus and Prunus necrotic ringspot virus

Massart

Brostaux²,

Barbarossa

et al. 2008

Eur J Plant Pathol

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“…Interlaboratory comparisons (ring analyses) have been applied commonly in food control, veterinary, forensic, and soil studies to evaluate, for example, Salmonella diagnostic accuracy (25), virus isolation (18), enzyme-linked immmunosorbent assay methods (2), mitochondrial DNA sequencing (30), soil microbial biomass C (3), and quantitative PCR (QPCR) (11). Ring analyses assessing the reproducibility of DNA extraction and subsequent community analyses between different laboratories have not been carried out so far in environmental microbial ecology.…”

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confidence: 99%

Impacts of Inter- and Intralaboratory Variations on the Reproducibility of Microbial Community Analyses

Pan

Bodrossy

Frenzel

et al. 2010

Appl Environ Microbiol

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With the advent of molecular biological techniques, especially next-generation sequencing and metagenomics, the number of microbial biogeography studies is rapidly increasing. However, these studies involve the synthesis of data generated by different laboratories using different protocols, chemicals, etc., all with inherent biases. The aim of this study was to assess inter-as well as intralaboratory variations in microbial community composition when standardized protocols are applied to a single soil sample. Aliquots from a homogenized soil sample from a rice field in Italy were sent to five participating laboratories. DNA was extracted by two investigators per laboratory using an identical protocol. All DNA samples were sent to one laboratory to perform DNA quantification, quantitative PCR (QPCR), and microarray and denaturing gradient gel electrophoresis (DGGE) analyses of methanotrophic communities. Yields, as well as purity of DNA, were significantly different between laboratories but in some cases also between investigators within the same laboratory. The differences in yield and quality of the extracted DNA were reflected in QPCR, microarray, and DGGE analysis results. Diversity indices (Shannon-Wiener, evenness, and richness) differed significantly between laboratories. The observed differences have implications for every project in which microbial communities are compared in different habitats, even if assessed within the same laboratory. To be able to make sensible comparisons leading to valid conclusions, intralaboratory variation should be assessed. Standardization of DNA extraction protocols and possible use of internal standards in interlaboratory comparisons may help in rendering a "quantifiable" bias.

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Foot-and-mouth disease virus: A first inter-laboratory comparison trial to evaluate virus isolation and RT-PCR detection methods

Cited by 36 publications

References 24 publications

A Continuous Bovine Kidney Cell Line Constitutively Expressing Bovine α_Vβ₆Integrin Has Increased Susceptibility to Foot-and-Mouth Disease Virus

A Continuous Bovine Kidney Cell Line Constitutively Expressing Bovine α_Vβ₆Integrin Has Increased Susceptibility to Foot-and-Mouth Disease Virus

Inter-laboratory evaluation of a duplex RT-PCR method using crude extracts for the simultaneous detection of Prune dwarf virus and Prunus necrotic ringspot virus

Impacts of Inter- and Intralaboratory Variations on the Reproducibility of Microbial Community Analyses

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Foot-and-mouth disease virus: A first inter-laboratory comparison trial to evaluate virus isolation and RT-PCR detection methods

Cited by 36 publications

References 24 publications

A Continuous Bovine Kidney Cell Line Constitutively Expressing Bovine αVβ6Integrin Has Increased Susceptibility to Foot-and-Mouth Disease Virus

A Continuous Bovine Kidney Cell Line Constitutively Expressing Bovine αVβ6Integrin Has Increased Susceptibility to Foot-and-Mouth Disease Virus

Inter-laboratory evaluation of a duplex RT-PCR method using crude extracts for the simultaneous detection of Prune dwarf virus and Prunus necrotic ringspot virus

Impacts of Inter- and Intralaboratory Variations on the Reproducibility of Microbial Community Analyses

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A Continuous Bovine Kidney Cell Line Constitutively Expressing Bovine α_Vβ₆Integrin Has Increased Susceptibility to Foot-and-Mouth Disease Virus

A Continuous Bovine Kidney Cell Line Constitutively Expressing Bovine α_Vβ₆Integrin Has Increased Susceptibility to Foot-and-Mouth Disease Virus