Foot-and-mouth disease polyvalent oil vaccines inoculated repeteadly in cattle do not induce detectable antibodies to non-structural proteins when evaluated by various assays

Espinoza, Ana Maria; Maradei, Eduardo; Mattion, Nora; G, Cadenazzi; Maddonni, Gabriela; Robiolo, Blanca; Torre, J.L. La; Bellinzoni, Rodolfo; Smitsaart, Eliana

doi:10.1016/j.vaccine.2004.05.007

Cited by 9 publications

(7 citation statements)

References 17 publications

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“…Some vaccines, though produced a strong antibody response to structural proteins (data not shown), they apparently were not completely purified from NSP contaminants as evident in this study. It has been shown that administration of FMD vaccine manufactured using appropriate antigen purification methods, even with high antigenic payload, allows differentiation of infected animals using the available NSP antibody detection systems [19]. In India, although the supply of NSP-free vaccines for the publicly funded control program is currently not required to be free from NSP, there is a need to regulate this for the unambiguous interpretation of NSP serology data while assessing the impact of the control program in the long run.…”

Section: Discussionmentioning

confidence: 99%

A multi-species indirect ELISA for detection of non-structural protein 3ABC specific antibodies to foot-and-mouth disease virus

et al. 2015

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Reliable diagnostic tests that are able to distinguish infected from vaccinated animals are a critical component of regional control programs for foot-and-mouth disease (FMD) in the affected countries. Non-structural protein (NSP) serology based on the 3ABC protein has been widely used for this purpose, and several kits are commercially available worldwide. This report presents the development of a 3ABC-antigen-based indirect ELISA, employing a peroxidase-conjugated protein G secondary antibody that can detect antibodies from multiple species. Recombinant 3ABC protein was expressed in insect cells and purified using affinity column chromatography. Using this protein, an indirect ELISA was developed and validated for the detection of NSP antibodies in serum samples collected from animals with different status of FMD. Diagnostic sensitivity and specificity were found to be 95.8 (95 % CI: 92.8-97.8) and 97.45 % (95 % CI: 94.8-99.0), respectively. The in-house ELISA compared well with the commercially available prioCHECK FMDV NS-FMD kit, with a high agreement between the tests, as determined by the kappa coefficient, which was 0.87. The in-house ELISA showed higher sensitivity for detecting vaccinated and subsequently infected animals, when compared to the reference test. Both of the tests were able to detect NSP antibodies as early as 7-8 days after experimental infection.

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Section: Discussionmentioning

confidence: 99%

A multi-species indirect ELISA for detection of non-structural protein 3ABC specific antibodies to foot-and-mouth disease virus

et al. 2015

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“…2,9,10 However, after an outbreak, during proof-of-freedom testing, the prevalence of infected animals can decrease quickly to close to zero. Results from this study indicate that the proportion of animals with detectable concentrations of vaccineinduced anti-NSP antibodies would be very low.…”

Section: Discussionmentioning

confidence: 99%

“…Results from this study indicate that the proportion of animals with detectable concentrations of vaccineinduced anti-NSP antibodies would be very low. 2,9,10 However, after an outbreak, during proof-of-freedom testing, the prevalence of infected animals can decrease quickly to close to zero. Low prevalences have a major effect on the positive predictive value of a diagnostic test, even if it has a high specificity.…”

Section: Discussionmentioning

confidence: 99%

Development and validation of a 3ABC antibody ELISA in Australia for foot and mouth disease

et al. 2014

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The 3ABC ELISA identified field and experimentally infected animals, as well as vaccinated and subsequently infected animals. Diagnostic sensitivity and specificity estimates for other FMD NSP tests are comparable with the results obtained in this study. This NSP ELISA was found to be 'fit for purpose' as a screening assay at the herd level to detect viral infection and also to substantiate absence of infection.

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“…Specificity should take the highest priority here because the quantitative EITB is designed as a confirmatory test following ELISA and it is desired to have a low false positivity rate. The NSP ELISA tests have specificity problems and should be complemented by an alternative assay such as EITB (Espinoza et al, 2004). In our study, a few weak positives detected in the first NSP test were evaluated together with the sequential tests and quantitative EITB.…”

Section: Discussionmentioning

confidence: 99%

Improvement of Enzyme-Linked Immunoelectrotransfer Blot Assay by Quantitative Approach for Foot-and-Mouth Disease diagnosis

Çokçalışkan

PARLAK

Sareyyüpoğlu³

et al. 2022

Harran Üniversitesi Veteriner Fakültesi Dergisi

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Foot-and-mouth disease (FMD) is a highly contagious animal disease that causes devastating economic losses. The trade of live animals and derived products is only possible if the exporting country is free from disease, according to the World Organisation for Animal Health (OIE) code for FMD. One of the most important ways to prove freedom from disease is based on the measurement of antibody levels to non-structural proteins (NSP) of FMD virus in the sampled target population. For detection of the disease status of a herd, mass screening and assays such as Enzyme-linked immunosorbent assay (ELISA) and Enzyme-Linked Immunoelectrotransfer Blot Assay (EITB) were developed and described in the OIE diagnostic manual. In this study, recombinant FMDV NS proteins were produced and tested with panels of sera that were collected from both uninfected and naturally infected animals using a quantitative Western blot assay as an improved EITB which enables numerical documentation and statistical analysis. NSP band intensities were used to determine the cut-off values, differentiating infected from non-infected animals and revealing variable sensitivity among the different NSPs. The quantitative EITB results also showed a correlation with the NSP-ELISA results.

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Foot-and-mouth disease polyvalent oil vaccines inoculated repeteadly in cattle do not induce detectable antibodies to non-structural proteins when evaluated by various assays

Cited by 9 publications

References 17 publications

A multi-species indirect ELISA for detection of non-structural protein 3ABC specific antibodies to foot-and-mouth disease virus

A multi-species indirect ELISA for detection of non-structural protein 3ABC specific antibodies to foot-and-mouth disease virus

Development and validation of a 3ABC antibody ELISA in Australia for foot and mouth disease

Improvement of Enzyme-Linked Immunoelectrotransfer Blot Assay by Quantitative Approach for Foot-and-Mouth Disease diagnosis

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