Foodborne Outbreak Caused by Staphylococcus aureus: Phenotypic and Genotypic Characterization of Strains of Food and Human Sources

Colombari, Viviane; Mayer, Mariana Del Ben; Laicini, Zaira M.; Mamizuka, Elsa M.; Franco, Bernadette D.G.M.; Destro, Maria Teresa; Landgraf, Mariza

doi:10.4315/0362-028x-70.2.489

Cited by 57 publications

(39 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…aureus. Furthermore, food handlers are important source of staphylococcal food contamination in restaurants and food outlets (Colombari et al, 2007). The presence of Staph.…”

Section: Resultsmentioning

confidence: 99%

Cooked poultry meat and products as a potential source of some food poisoning bacteria

El-Malek¹

2017

IOSR JESTFT

View full text Add to dashboard Cite

“…aureus. Furthermore, food handlers are important source of staphylococcal food contamination in restaurants and food outlets (Colombari et al, 2007). The presence of Staph.…”

Section: Resultsmentioning

confidence: 99%

Cooked poultry meat and products as a potential source of some food poisoning bacteria

El-Malek¹

2017

IOSR JESTFT

View full text Add to dashboard Cite

“…In a few of these patients, S. aureus can be isolated from a stool sample, and in an even far lower number of cases, the isolated strain can be matched to isolates collected from foodstuff, environmental samples, or food handlers. In many outbreak reports, stool samples are missing or negative, thus forcing the authors to try to evaluate the relevance of numerous enterotoxigenic staphylococcal isolates collected from different sources (Do Carmo et al, 2004;Colombari et al, 2007;Schmid et al, 2009 DOI: 10.1089DOI: 10. /fpd.2013DOI: 10.…”

mentioning

confidence: 99%

Outbreak of Staphylococcal Food Poisoning Due to SEA-ProducingStaphylococcus aureus

Johler

Tichaczek-Dischinger

Rau

et al. 2013

Foodborne Pathogens and Disease

View full text Add to dashboard Cite

In 2008, 150 people gathered for a wedding celebration in Baden-Württemberg, Germany. Three hours after ingestion of a variety of foods including pancakes filled with minced chicken, several guests exhibited symptoms of acute gastroenteritis such as vomiting, diarrhea, fever, and ague. Twelve guests were reported to have fallen ill, with nine of these seeking medical care in hospitals. At least four patients were admitted to the hospital and received inpatient treatment, among them a 2-year-old child and a woman in the 4th month of pregnancy. Within 24 h of the event, an investigative team collected a variety of samples including refrigerated leftovers, food in the storage unit of the caterer, nasal swabs of the caterer, as well as 21 environmental swabs. Five stool samples from patients were provided by the hospitals. Staphylococcus aureus isolates were gathered from eight samples, among them nasal swabs of the caterer, food samples, and one stool sample. Fourier transform-infrared spectroscopy was used for species identification and for primary clustering of the isolates in a similarity tree. The isolates were further characterized by spa typing and pulsed-field gel electrophoresis, and a DNA microarray was used to determine the presence/absence of genes involved in virulence and antimicrobial resistance. We were able to match an enterotoxigenic strain from the stool sample of a patient to isolates of the same strain obtained from food and the nasal cavity of a food handler. The strain produced the enterotoxin SEA and the toxic shock syndrome toxin-1, and was also found to exhibit the genes encoding enterotoxins SEG and SEI, as well as the enterotoxin gene cluster egc. This is one of only a few studies that were able to link a staphylococcal food poisoning outbreak to its source. In 2008, 150 people gathered for a wedding celebration in Baden-Wü rttemberg, Germany. Three hours after ingestion of a variety of foods including pancakes filled with minced chicken, several guests exhibited symptoms of acute gastroenteritis such as vomiting, diarrhea, fever, and ague. Twelve guests were reported to have fallen ill, with nine of these seeking medical care in hospitals. At least four patients were admitted to the hospital and received inpatient treatment, among them a 2-year-old child and a woman in the 4th month of pregnancy.Within 24 h of the event, an investigative team collected a variety of samples including refrigerated leftovers, food in the storage unit of the caterer, nasal swabs of the caterer, as well as 21 environmental swabs. Five stool samples from patients were provided by the hospitals. Staphylococcus aureus isolates were gathered from eight samples, among them nasal swabs of the caterer, food samples, and one stool sample. Fourier transforminfrared spectroscopy was used for species identification and for primary clustering of the isolates in a similarity tree. The isolates were further characterized by spa typing and pulsed-field gel electrophoresis, and a DNA microarray was used to determin...

show abstract

“…Nasal carriage rate as low as 0.77% has also been reported [19]. The source of the Staph aureus outbreak that affected 180 individuals in Brazil was also the food handlers carrying Staph aureus in their throats [20]. According to Bassyonni et al [21], ecological differences of the study population may be responsible for the variations in nasal carriage rates.…”

Section: Discussionmentioning

confidence: 95%

Asymptomatic Carriage of Plasmid-Mediated Multidrug-Resistant Staphylococcus aureus in Nasal Tracts of Persons in a Semi-Urban Area of Nigeria

Isibor¹,

Otabor²

2014

AJLS

View full text Add to dashboard Cite

Abstract:Staphylococcus aureus remains one of the most important opportunistic bacterial pathogens of man and strains resistant to available drug classes have become more and more prevalent in clinical and community settings. The aim of this study was to determine the nasal carriage rates of multidrug -resistant (MDR) Staph aureus in food vendors and hospital workers in Ekpoma, Nigeria, and to assess whether resistance was plasmid -mediated. With the use of sterile swab sticks (Sterilin, UK), one hundred nasal swabs were obtained from consenting volunteers. Each swab was streaked on mannitol salt agar (Difco, Detroit MI) and incubated at 37 0 C for 24hrs. Colonies growing on medium were Gram -stained and tested for catalase, coagulase and mannitol fermentation. Antibiotic susceptibility tests were performed using the Kirby-Bauer disc diffusion technique, and results were interpreted using the Clinical and Laboratory Standard Institute's guidelines. Results showed that 32 (32%) of the 100 specimens screened yielded Staph aureus, with 18 (36%) and 14 (28%) of the isolates recovered from hospital workers and food vendors, respectively. The prevalence rate in males (40%) compared with females (24%) was not statistically significant (P>0.05), using Student's t-test analysis. All the Staph aureus isolates were susceptible to Augmentin. The highest resistance rates of 65.6%, 59.3% and 50.0% were recorded for Amoxycillin, Ofloxacin and Oxacillin respectively, while low resistance rates were recorded for Gentamicin (9.38%) and Cefuroxime (15.6%). Of the 17 (53%) MDR isolates of Staph aureus, only 4 (23.5%) remained MDR after curing with 0.1mg/ml acridine orange solution. Observance of personal hygiene can reduce the level of contamination or infection of carriers thereby reducing the risk of infection for others in the community, while rational use of antibiotics by health professionals and end users could help to curb the rising trend of antibiotic resistance within the community.

show abstract

Foodborne Outbreak Caused by Staphylococcus aureus: Phenotypic and Genotypic Characterization of Strains of Food and Human Sources

Cited by 57 publications

References 16 publications

Cooked poultry meat and products as a potential source of some food poisoning bacteria

Cooked poultry meat and products as a potential source of some food poisoning bacteria

Outbreak of Staphylococcal Food Poisoning Due to SEA-ProducingStaphylococcus aureus

Asymptomatic Carriage of Plasmid-Mediated Multidrug-Resistant Staphylococcus aureus in Nasal Tracts of Persons in a Semi-Urban Area of Nigeria

Contact Info

Product

Resources

About