Food restriction alters pregnancy-associated  changes in IGF and IGFBP in the guinea pig

Sohlström, Annica; Katsman, Arkadi; Kind, Karen L.; Roberts, Claire T.; Owens, Philip; Robinson, J. S.; Owens, Julie A.

doi:10.1152/ajpendo.1998.274.3.e410

Cited by 47 publications

(86 citation statements)

References 21 publications

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“…IGFBP-4 was present as a 25 kDa band. We did not detect the additional band that has been described for adult guinea pigs and is thought to include IGFBP-1 protein (Sohlström et al 1998b).…”

Section: Proteins In Plasma and Amniotic Fluidcontrasting

confidence: 51%

“…The most abundant IGFBP in adult guinea pig plasma is IGFBP-3 (Sohlström et al 1998b), and this was present in fetal plasma as a 40-35 kDa doublet corresponding to the glycosylated and nonglycosylated forms. However, the principal IGFBP in fetal guinea pig plasma was identified by molecular mass and immunoblotting as IGFBP-2 (Fig.…”

Section: Proteins In Plasma and Amniotic Fluidmentioning

confidence: 99%

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Altered expression of IGFs and IGF-binding proteins during intrauterine growth restriction in guinea pigs

Carter¹,

Kingston²,

Han³

et al. 2005

Journal of Endocrinology

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The IGF system is one of the most important endocrine and paracrine growth factor systems that regulate fetal and placental growth. We hypothesized that intrauterine growth restriction (IUGR) in guinea pigs is mediated by the altered expression of IGFs and/or IGF binding protein (BP) mRNAs in tissues and is related to growth of specific tissues. IUGR was induced by unilateral uterine artery ligation on day 30 of gestation, and fetal plasma, amniotic fluid and tissue samples were collected at 55-57 days (term about 68 days) from paired IUGR and control fetuses (n=6). Western ligand blotting and immunoblotting were used to compare IGFBP levels in plasma and amniotic fluid. Total RNA was extracted from placenta and fetal tissues, and the relative abundance of IGF-II and IGFBP-1-6 mRNA was determined by Northern blotting, using species-specific probes where available. IUGR fetuses had decreased (P<0·01, by Student's t-test) placental weight and body weight with an increase in the brain:liver weight ratio. The principal IGFBPs in fetal plasma migrated at 40-35, 30 and 25 kDa and were identified as IGFBP-3, -2 and -4 respectively. IUGR was associated with elevated plasma IGFBP-2 and IGFBP-4 and reduced IGFBP-3 levels. IGFBPs were detected at low levels in amniotic fluid of control fetuses but at higher levels in IUGR fetuses. In IUGR placentae, there was a small increase in IGFBP-4 mRNA (P<0·05). IGFBP-2 mRNA increased (P<0·001) in liver of IUGR fetuses. IGF-II and IGFBP mRNA expression did not change in fetal muscle. The results are consistent with reduced IGF action, directly or through inhibition by IGFBPs, particularly by circulating and tissue IGFBP-2, as a potential causal factor in decreased growth of the placenta and certain fetal tissues.

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Section: Proteins In Plasma and Amniotic Fluidcontrasting

confidence: 51%

Section: Proteins In Plasma and Amniotic Fluidmentioning

confidence: 99%

Altered expression of IGFs and IGF-binding proteins during intrauterine growth restriction in guinea pigs

Carter¹,

Kingston²,

Han³

et al. 2005

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…However, unlike other mammals, the somatomedin axis appears to be completely uncoupled from GH, since hypophysectomy or GH administration does not alter IGF-I or IGF-II concentrations (Daughaday et al 1986b, Daughaday 1992. The factors responsible for IGF-I induction are currently unknown, although IGF concentrations are affected by pregnancy and malnutrition (Sohlstrom et al 1998). Growth in guinea pigs may thus resemble several physiological and pathophysiological states in humans, including GH-deficient patients with normal IGF-I levels (Ho & Hoffman 1995).…”

Section: Guinea Pigsmentioning

confidence: 99%

Growth hormone resistance: clinical states and animal models

Hull¹,

Harvey²

1999

Journal of Endocrinology

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GH exerts pleiotropic effects on growth and metabolism through the GH receptor. A deficiency in the GH receptor gene is thus associated with GH resistance and dwarfism. Complete GH resistance in humans, or Laron syndrome, has been associated with numerous inherited defects in the GH receptor, including point mutations, complete or partial gene deletions, and splice site alterations. Analysis of the GH receptor genes of these patients has provided considerable insight into structure-function relationships of the GH receptor. However, the relative rarity of this disease and the obvious difficulties involved in human research have prompted a search for an animal model of GH resistance. Numerous models have been proposed, including the sex-linked dwarf chicken, the guinea pig, and the Laron mouse. In this review, the characteristics and etiology of Laron syndrome and these animal models will be discussed. The insight provided by these disorders into the roles and mechanism of action of GH will also be reviewed.

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“…Therefore, there is no obvious attempt of the guinea-pig placenta to try and compensate for undernutrition. At a mechanistic level, expression of IGF-I and -II, and the ratio of the IGFs to IGF binding proteins, is reduced in response to feed restriction (Sohlstrom et al 1998;Roberts et al 2001bRoberts et al , 2002Olausson and Sohlstrom 2003). Insulinlike growth factor levels are correlated with the extent of villous branching and are inversely correlated with thickness of the trophoblast barrier (Roberts et al 2001b(Roberts et al , 2002, similar to changes seen in IGF-II-deficient mice (Constancia et al 2002;Sibley et al 2004).…”

Section: Altered Placental Development In Underand Overnourished Animmentioning

confidence: 76%

Placental function in development and disease

Cross

2006

Reprod. Fertil. Dev.

112

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Abstract. The placenta is an organ that clinicians and embryologists would all agree is important for pregnancy success. Unfortunately, however, they too often ignore it when they are exploring causes for embryonic, fetal and perinatal complications. The core function of the placenta is to mediate the transport of nutrients between the maternal and fetal circulation, but it also has critical endocrine functions that alter different maternal physiological systems in order to sustain pregnancy. Both its development and ongoing functions can be dynamically regulated by environmental factors, including nutrient status and tissue oxygenation. In recent years, mainstream attention has begun to shift onto the placenta and it is now becoming clear that placental pathology is associated with several complications in human and animal pregnancies, including embryonic lethality, fetal growth restriction, pre-eclampsia and the high rates of fetal deaths observed after nuclear transfer (cloning).

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Food restriction alters pregnancy-associated changes in IGF and IGFBP in the guinea pig

Cited by 47 publications

References 21 publications

Altered expression of IGFs and IGF-binding proteins during intrauterine growth restriction in guinea pigs

Altered expression of IGFs and IGF-binding proteins during intrauterine growth restriction in guinea pigs

Growth hormone resistance: clinical states and animal models

Placental function in development and disease

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