2021
DOI: 10.51791/njap.v48i4.2994
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Food insecurity access scale and dietary score as proxy indicators of nutritional status among backyard livestock households during COVID-19 pandemic in Southwest Nigeria

Abstract: Food insecurity and dietary diversity of households are important nutrition outcome which have been found reliable in assessing the dietary intake of a population during a determined period and they have been used as indicators of food security among households. A facility based cross-sectional study design was conducted on 374 households that were involved in backyard livestock production in Southwest Nigeria during COVID-19 pandemic lockdown. Household dietary diversity (HDD) was measured using 12 different … Show more

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“…The DNA was quantified, assessed and then diluted to 100 mg/μL DNA prior to polymerase chain reaction (PCR) set up. As it was detailed in [17], 25 μL volume of 2.0 μL of 50 mM mgcl2 (Biolino), 0.2 μL 500 μ tag DNA polymerase and 2.0 μL of 2.5 mM dNTPs (Biolino); 1.0 μL of 10 μM each primer, 16.05 μ of 500 ml and 1.0 μL DMSO (dimethly sulfoxide) were used to carry out the PCR amplification with DEPC-treated water (in vitrogen corporation). Routine PCR cycling profile was used for the reaction and involved 940C cycle for 2 minutes, another 40 cycles of 2 minutes and a 720C 5 minute final extension [17].…”
Section: Methodsmentioning
confidence: 99%
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“…The DNA was quantified, assessed and then diluted to 100 mg/μL DNA prior to polymerase chain reaction (PCR) set up. As it was detailed in [17], 25 μL volume of 2.0 μL of 50 mM mgcl2 (Biolino), 0.2 μL 500 μ tag DNA polymerase and 2.0 μL of 2.5 mM dNTPs (Biolino); 1.0 μL of 10 μM each primer, 16.05 μ of 500 ml and 1.0 μL DMSO (dimethly sulfoxide) were used to carry out the PCR amplification with DEPC-treated water (in vitrogen corporation). Routine PCR cycling profile was used for the reaction and involved 940C cycle for 2 minutes, another 40 cycles of 2 minutes and a 720C 5 minute final extension [17].…”
Section: Methodsmentioning
confidence: 99%
“…Two ISSR primers used for this analysis were: (AG) 9C 5'-AGA GAG AGA GAG AGA GAG C-3' and (GA) 9C 5'-GAG AGA GAG AGA GAG AGA C-3' [17]. Fingerprint analyses was carried out using py Elph version 1.4.…”
Section: Methodsmentioning
confidence: 99%