Food-grade expression system of Lactobacillus plantarum using β-galactosidase small subunit as selection marker and lactose as screening condition

Sugar Utilization-Associated Food-Grade Selection Markers in Lactic Acid Bacteria and Yeast

“…β gal gene from Lactobacillus delbrueckii subsp. bulgaricus ( Hashiba et al 1992 ; Lin et al 1996 ), lacF gene from Lactococcus lactis ( Platteeuw et al 1996 ; Cao et al 2019; Tagliavia and Nicosia 2019 ), lacZ gene from Lactobacillus acidophilus ( Deng et al 2011 ), and lacLM gene from Lactiplantibacillus plantarum ( Wang et al 2023 ) are all genes encoding β-galactosidase. Studies have shown that these genes can be used as selection markers for lactose metabolism.…”

“…After successful construction, the recombinant microorganisms and their products can be combined with some commonly used milk-based media to produce various healthy, nutritious, or better-tasting foods. Using the lactose promoter in plasmid vectors with lactose-selective markers and inducing with lactose can help achieve good expression ( Wang et al 2023 ); this construction can exhibit dual effects of the screening of exogenous lactose and lactose-selective marker expression. Selecting the target host bacteria would be a better and more straightforward method than using lactic acid bacteria with only β-galactosidase (lacF) activity, knocking out its lacF gene and constructing it into a plasmid vector ( MacCormick et al 1995 ; Platteeuw et al 1996 ).…”

“…Selecting the target host bacteria would be a better and more straightforward method than using lactic acid bacteria with only β-galactosidase (lacF) activity, knocking out its lacF gene and constructing it into a plasmid vector ( MacCormick et al 1995 ; Platteeuw et al 1996 ). There levant research analysis results show that the selection marker of β gal is more applicable to the integration with chromosomes ( Hashiba et al 1992 ; Lin et al 1996 ) rather than exist in the host cell with modality of recombinant plasmid; the lacLM coding protein is the heterodimer ( Wang et al 2023 ) formed by large subunit and small subunit coded by lacL and lacM gene. It is undoubtedly difficult to construct such recombinant carrier on account of relatively long coding gene; the plasmid vectors labeled with the lacG gene exhibits incompatibility with wild-type lactic acid bacterial strains ( Takala et al 2003 ).…”