Food entrains clock genes but not metabolic genes in the liver of suprachiasmatic nucleus lesioned rats

Sabath, Elizabeth; Salgado‐Delgado, Roberto; Guerrero‐Vargas, Natalí N.; Guzmán-Ruiz, Mara; Basualdo, María del Carmen; Escobar, Carolina; Buijs, Ruud M.

doi:10.1016/j.febslet.2014.06.045

Cited by 14 publications

(10 citation statements)

References 36 publications

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“…Moreover, in intact animals NAMPT rhythms were also lost when feeding times were not in line with the L/D cycle (Salgado-Delgado et al 2013). Since SCN-lesioned animals in the Sabath et al (Sabath et al 2014) study were also housed in L/D conditions, these results agree with the idea that the L/D cycle determines NAMPT rhythmicity, instead of feeding rhythms or the molecular clock.…”

Section: Nampt Retains More Rhythmicity Than the Core Clock After Scn Lesionssupporting

confidence: 68%

“…Remarkably, in SCN-lesioned animals on a feeding schedule, only hepatic NAMPT levels lost their rhythmicity; whereas, all of the other clock genes investigated remained rhythmic in these conditions (Sabath et al 2014). These results show NAMPT levels are not directly regulated by food intake; in fact, the enforced feeding schedule seems to disturb its rhythmicity, since in fasted conditions NAMPT levels in SCN-lesioned animals retained their rhythmicity.…”

Section: Nampt Retains More Rhythmicity Than the Core Clock After Scn Lesionsmentioning

confidence: 93%

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Thermal lesions of the SCN do not abolish all gene expression rhythms in rat white adipose tissue, NAMPT remains rhythmic

Spek

Foppen

Fliers

et al. 2021

Chronobiology International

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Obesity and type 2 diabetes mellitus are major health concerns worldwide. In obese-type 2 diabetic patients, the function of the central brain clock in the hypothalamus, as well as rhythmicity in white adipose tissue (WAT), are reduced. To better understand how peripheral clocks in white adipose tissue (WAT) are synchronized, we assessed the importance of the central brain clock for daily WAT rhythms. We compared gene expression rhythms of core clock genes (Bmal1, Per2, Cry1, Cry2, RevErbα, and DBP) and metabolic genes (SREBP1c, PPARα, PPARγ, FAS, LPL, HSL, CPT1b, Glut4, leptin, adiponectin, visfatin/NAMPT, and resistin) in epididymal and subcutaneous white adipose tissue (eWAT and sWAT) of SCN-lesioned and sham-lesioned rats housed in regular L/D conditions. Despite complete behavioral and hormonal arrhythmicity, SCN lesioning only abolished Cry2 and DBP rhythmicity in WAT, whereas the other clock gene rhythms were significantly reduced, but not completely abolished. We observed no major differences in the effect of SCN lesions between the two WAT depots. In contrast to clock genes, all metabolic genes lost their daily rhythmicity in WAT, with the exception of NAMPT. Interestingly, NAMPT rhythmicity was even less affected by SCN lesioning than the core clock genes, suggesting that it is either strongly coupled to the remaining rhythmicity in clock gene expression, or very sensitive to other external rhythmic factors. The L/D cycle could be such a rhythmic external factor that generates modulating signals by photic masking via the intrinsic photosensitive retinal ganglion cells in combination with the autonomic nervous system. Our findings indicate that in normal weight rats, gene expression rhythms in WAT can be maintained independent of the central brain clock.

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Section: Nampt Retains More Rhythmicity Than the Core Clock After Scn Lesionssupporting

confidence: 68%

Section: Nampt Retains More Rhythmicity Than the Core Clock After Scn Lesionsmentioning

confidence: 93%

Thermal lesions of the SCN do not abolish all gene expression rhythms in rat white adipose tissue, NAMPT remains rhythmic

Spek

Foppen

Fliers

et al. 2021

Chronobiology International

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“…Our results show that SW induced a large phase advance of Bmal1 mRNA expression in the liver, which was restored on RD7; although, with a lower amplitude than that of the CTRL. Since hepatic Bmal1 expression is mainly driven by food intake rather than by the SCN (Dang et al, 2016; Sabath et al, 2014), it is likely that the phase advance and resynchronization rate reflect the hepatic adjustments to feeding behavior.…”

Section: Discussionmentioning

confidence: 99%

Differential Recovery Speed of Activity and Metabolic Rhythms in Rats After an Experimental Protocol of Shift-Work

et al. 2019

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The circadian system drives the temporal organization of body physiology in relation to the changing daily environment. Shift-work (SW) disrupts this temporal order and is associated with the loss of homeostasis and metabolic syndrome. In a rodent model of SW based on forced activity in the rest phase for 4 weeks, we describe the occurrence of circadian desynchrony, as well as metabolic and liver dysfunction. To provide better evidence for the impact of altered timing of activity, this study explored how long it takes to recover metabolic rhythms and behavior. Rats were submitted to experimental SW for 4 weeks and then were left to recover for one week. Daily locomotor activity, food intake patterns, serum glucose and triglycerides, and the expression levels of hepatic Pparα, Srebp-1c, Pepck, Bmal1 and Per2 were assessed during the recovery period and were compared with expected data according to a control condition. SW triggered the circadian desynchronization of all of the analyzed parameters. A difference in the time required for realignment was observed among parameters. Locomotor activity achieved the expected phase on day 2, whereas the nocturnal feeding pattern was restored on the sixth recovery day. Daily rhythms of plasma glucose and triglycerides and of Pparα, Pepck and Bmal1 expression in the liver resynchronized on the seventh day, whereas Srebp-1c and Per2 persisted arrhythmic for the entire recovery week. SW does not equally affect behavior and metabolic rhythms, leading to internal desynchrony during the recovery phase.

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“…It is possible that circadian variation in 5-FU concentration is due, at least in part, directly to factors associated with time-of-day such as activity, food, light, or sleep, rather than—or interacting with—an intrinsic periodicity of metabolism [ 25 ]. This could account for the variability in results seen across series assessing the relationship of 5-FU concentrations with time of day, and would also increase the likelihood of detecting circadian variation in our series, where all patients were housed in a clinical research unit where meal timing was fairly uniform and efforts were made to maintain quiet at night.…”

Section: Discussionmentioning

confidence: 99%

Circadian variation in plasma 5-fluorouracil concentrations during a 24 hour constant-rate infusion

et al. 2015

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BackgroundVarying the rate of continuous intravenous infusions of 5-fluorouracil (5FU) chemotherapy over a 24-hour period has been reported to improve patient outcomes. It has been hypothesized that circadian variation in drug disposition is a contributing factor. We analyzed 5-FU concentrations during a 24-hour continuous 5-FU infusion.MethodsSixty-four subjects with advanced malignancies including pancreatic, hepatocellular, colorectal as well as other epithelial malignancies and either abnormal hepatic or renal function were treated on a phase I and pharmacokinetic study of weekly 24-hour intravenous infusions of 5-FU and leucovorin. No other concomitant anticancer therapy was administered. Blood samples were collected every three hours from 61 subjects for measurement of plasma 5-FU during the first two weekly infusions.ResultsAfter adjusting for differences in dose, elapsed time from start of infusion and infusion number (2 versus 1), mean 5-FU concentration was highest at 6 am and lowest at 3 pm, with an overall change in the mean from 3 pm to 6 am of +20 percent (95% CI = 12–28%). However, this variation in mean concentration associated with time of day was comparable in magnitude to the between-patient differences, within-patient differences between infusions, and the residual variation within infusion (coefficient of variation = 21%).ConclusionsOur data show systematic variation by time of day in plasma concentrations of 5-FU administered at a constant rate over 24 hours, but it is small compared to the total variation in plasma concentration contributed by other sources. Circadian variation in men was more pronounced than in women.

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Food entrains clock genes but not metabolic genes in the liver of suprachiasmatic nucleus lesioned rats

Cited by 14 publications

References 36 publications

Thermal lesions of the SCN do not abolish all gene expression rhythms in rat white adipose tissue, NAMPT remains rhythmic

Thermal lesions of the SCN do not abolish all gene expression rhythms in rat white adipose tissue, NAMPT remains rhythmic

Differential Recovery Speed of Activity and Metabolic Rhythms in Rats After an Experimental Protocol of Shift-Work

Circadian variation in plasma 5-fluorouracil concentrations during a 24 hour constant-rate infusion

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