Food antigen causes TH2-dependent enteropathy followed by tissue repair in T-cell receptor transgenic mice

Nakajima‐Adachi, Haruyo; Ebihara, Ayumi; Kikuchi, Akira; Ishida, Tsuyoshi; Sasaki, Kiyomi; Hirano, K; Watanabe, Hiroko; Asai, K.; Takahashi, Yoshimasa; Kanamori, Yutaka; Shimojo, Naoki; Matsuda, Hiroshi; Kohno, Yoichi; Hachimura, Satoshi; Kaminogawa, Shuichi

doi:10.1016/j.jaci.2006.01.016

Cited by 42 publications

(56 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with previous reports [13,15,16] , the mice in the OVA group, which were fed a diet containing 20% egg white, equivalent to approximately 10% OVA, displayed a reduced body weight at day 7 that was significantly lower than that of the control group ( Fig. 1 a).…”

Section: Dietary Fos Have a Moderate Protective Effect On Intestinal supporting

confidence: 91%

“…This transgenic mouse line possesses CD4 + T cells that express a T cell receptor specific for ovalbumin (OVA), a major allergen present in egg white. It has been reported that feeding an egg-whitecontaining diet to these mice ad libitum can induce intestinal and systemic Th2 cytokine responses as well as intestinal inflammation with weight loss attributed to IL-4-producing, antigen-specific CD4 + T cells [13][14][15][16] . In addition, both mesenteric lymph nodes (MLN) and Peyer's patches (PP) are key sites for the generation and maintenance of IL-4-producing CD4 + T cells in the development of food allergy [15] .…”

Section: T Cells Which Produce Both Th1 and Th2 Cytokines In The Intementioning

confidence: 99%

See 1 more Smart Citation

Dietary Fructo-Oligosaccharides Attenuate Early Activation of CD4<sup>+</sup> T Cells Which Produce both Th1 and Th2 Cytokines in the Intestinal Lymphoid Tissues of a Murine Food Allergy Model

Tsuda¹,

Arakawa²,

Ishii³

et al. 2017

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

Background: Fructo-oligosaccharides (FOS) are prebiotic agents with immunomodulatory effects involving improvement of the intestinal microbiota and metabolome. In this study, we investigated the cellular mechanisms through which FOS modulate intestinal antigen-specific CD4⁺ T cell responses in food allergy, using OVA23-3 mice. Methods: OVA23-3 mice were fed an experimental diet containing either ovalbumin (OVA) or OVA and FOS for 1 week. Body weight and mucosal mast cell protease 1 in the serum were measured as the indicator of intestinal inflammation. Single-cell suspensions were prepared from intestinal and systemic lymphoid tissues for cellular analysis. Cytokine production was measured by ELISA. Activation markers and intracellular cytokines in CD4⁺ T cells were analyzed by flow cytometry. Activated CD4⁺ T cells were purified to examine cytokine production. Results: Dietary intake of FOS provided moderate protection from the intestinal inflammation induced by the OVA-containing diet. FOS significantly reduced food allergy-induced Th2 cytokine responses in intestinal tissues but not in systemic tissues. FOS decreased OVA diet-induced IFN-γ⁺IL-4⁺ double-positive CD4⁺ T cells and early-activated CD45RB^highCD69⁺CD4⁺ T cells in the mesenteric lymph nodes. Furthermore, we confirmed that these CD45RB^highCD69⁺CD4⁺ T cells are able to produce high levels of IFN-γ and moderate level of IL-4, IL-10, and IL-13. Conclusions: Dietary intake of FOS during the development of food allergy attenuates the induction of intestinal Th2 cytokine responses by regulating early activation of naïve CD4⁺ T cells, which produce both Th1 and Th2 cytokines. Our results suggest FOS might be a potential food agent for the prevention of food allergy by modulating oral sensitization to food antigens.

show abstract

Section: Dietary Fos Have a Moderate Protective Effect On Intestinal supporting

confidence: 91%

Section: T Cells Which Produce Both Th1 and Th2 Cytokines In The Intementioning

confidence: 99%

Dietary Fructo-Oligosaccharides Attenuate Early Activation of CD4<sup>+</sup> T Cells Which Produce both Th1 and Th2 Cytokines in the Intestinal Lymphoid Tissues of a Murine Food Allergy Model

Tsuda¹,

Arakawa²,

Ishii³

et al. 2017

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Supernatants were collected for determination of histamine levels by an enzyme immunoassay kit (USCN Life Science, Double Lake, Missouri City, TX) as described by the manufacturer. Histamine contents were standardised to the weight of intestine tissue in each sample (Nakajima-Adachi et al 2006). …”

Section: Measurement Of Histamine Contentsmentioning

confidence: 99%

Induction of immune responses and allergic reactions in piglets by injecting glycinin

Cao

Meng

et al. 2016

Italian Journal of Animal Science

View full text Add to dashboard Cite

Glycinin, an antigenic glycoprotein found in soybeans, is the major cause of allergic reactions in young animals. The objective of this study was to evaluate the effects of a prior immunisation of sucking piglets with glycinin on their post-weaning growth performance, serum immunoglobulin contents, small intestinal histamine release and mucosal histology. Forty piglets (7 d of age) were randomly divided into four groups of 10 piglets each. Piglets of Group C (Control) received a physiological saline solution, Groups Im (Immunised) and Im þ S (Sensitised) were immunised twice with 500 lg/kg of glycinin at 7 and 21-d-old. All piglets were weaned at 23 d; Groups Im þ S and S were sensitised with 2500 lg/kg of glycinin at weaning. Compared with Group C, in Group S the average daily gain (ADG), average daily feed intake and gain:feed ratio were decreased, and serum levels of IgG and IgE were increased. Furthermore, in this group, the histamine levels in the duodenum and ileum were significantly decreased, and the structure of duodenal and ileal mucosa was severely damaged. On the contrary, in Groups Im and Im þ S the ADG was increased, serum IgE levels were decreased, intestinal histamine levels were increased and the intestinal mucosa was not damaged. These findings suggest that prior immunisation with glycinin can protect the structural integrity of the intestinal mucosal epithelia and alleviate allergic reactions in piglets.ARTICLE HISTORY

show abstract

“…Indeed, oral tolerance can be induced in spleen (SP) T cells from these mice when fed an OVA-containing diet over a long period [16,17]. It has also been reported that OVA23-3 mice produce OVA-specific antibodies in the serum and develop intestinal inflammation in a Th2-dependent manner after intake of an OVA-containing diet [18,19]. However, it remains unknown how intestinal commensal bacteria regulate oral tolerance and dietary antigen-induced allergic reactions in this mouse model.…”

Section: Introductionmentioning

confidence: 99%

Intestinal commensal bacteria promote T cell hyporesponsiveness and down-regulate the serum antibody responses induced by dietary antigen

Tsuda¹,

Hosono²,

Yanagibashi³

et al. 2010

Immunology Letters

Self Cite

View full text Add to dashboard Cite

Food antigen causes TH2-dependent enteropathy followed by tissue repair in T-cell receptor transgenic mice

Cited by 42 publications

References 26 publications

Dietary Fructo-Oligosaccharides Attenuate Early Activation of CD4<sup>+</sup> T Cells Which Produce both Th1 and Th2 Cytokines in the Intestinal Lymphoid Tissues of a Murine Food Allergy Model

Dietary Fructo-Oligosaccharides Attenuate Early Activation of CD4<sup>+</sup> T Cells Which Produce both Th1 and Th2 Cytokines in the Intestinal Lymphoid Tissues of a Murine Food Allergy Model

Induction of immune responses and allergic reactions in piglets by injecting glycinin

Intestinal commensal bacteria promote T cell hyporesponsiveness and down-regulate the serum antibody responses induced by dietary antigen

Contact Info

Product

Resources

About