“…Carnitine and acyl carnitines being chiral molecules, optical rotation (OR) and circular dichroism in the UV range (electronic circular dichroism, ECD) were measured; in the latter case, ECD has been used as a detector of enantiomeric separation, down the line of HPLC separation protocol, without the accuracy required in a dedicated ECD experiment. To the best of our knowledge, though, no one has ever measured vibrational circular dichroism (VCD); yet various forms of vibrational spectroscopy, including IR, Raman, and SERS (surface‐enhanced Raman spectroscopy) have been carried out and demonstrated to be useful . VCD has acquired increasing importance through the years in the chiroptical spectroscopies field and has permitted tackling several problems, including determination of the absolute configuration (AC), investigation of conformational properties, chiral recognition and conformation, and the role of biomolecules.…”