Food additive octyl gallate eliminates acrolein and inhibits bacterial growth in oil-rich food

Qiu, Caiyi; Lu, Yong; Gu, Hong; Jia, Mengwei; Wang, Jiaqi; Zhang, Qiuting; Lv, Lishuang

doi:10.1016/j.foodchem.2022.133546

Cited by 4 publications

(2 citation statements)

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“…OGA is already approved as a food additive by Food and Drug Administration in the USA and its safety and manufacturing methods have been established. Qiu et al, reported that OGA could be taken from edible oils and oil emulsions amounting to 100 mg/kg supporting that OGA is low toxicity to human health ( Qiu et al, 2022 ). This suggests that market-targeted dugs including OGA have good advantages for going to clinical practice smoothly.…”

Section: Discussionmentioning

confidence: 99%

“…We determined that alkyl gallates, particularly octyl gallate (OGA), showed potent CysE inhibitory activities. OGA has long been used as a food additive to prevent oxidation of lipid ( Qiu et al, 2022 ). It has also been reported the antibacterial activity of OGA against Gram-positive bacteria including methicillin resistance S. aureus (MRSA) and Bacillus subtilis .…”

Section: Introductionmentioning

confidence: 99%

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Alkyl gallates inhibit serine O-acetyltransferase in bacteria and enhance susceptibility of drug-resistant Gram-negative bacteria to antibiotics

Toyomoto,

Ono,

Shiba

et al. 2023

Front. Microbiol.

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A principal concept in developing antibacterial agents with selective toxicity is blocking metabolic pathways that are critical for bacterial growth but that mammalian cells lack. Serine O-acetyltransferase (CysE) is an enzyme in many bacteria that catalyzes the first step in l-cysteine biosynthesis by transferring an acetyl group from acetyl coenzyme A (acetyl-CoA) to l-serine to form O-acetylserine. Because mammalian cells lack this l-cysteine biosynthesis pathway, developing an inhibitor of CysE has been thought to be a way to establish a new class of antibacterial agents. Here, we demonstrated that alkyl gallates such as octyl gallate (OGA) could act as potent CysE inhibitors in vitro and in bacteria. Mass spectrometry analyses indicated that OGA treatment markedly reduced intrabacterial levels of l-cysteine and its metabolites including glutathione and glutathione persulfide in Escherichia coli to a level similar to that found in E. coli lacking the cysE gene. Consistent with the reduction of those antioxidant molecules in bacteria, E. coli became vulnerable to hydrogen peroxide-mediated bacterial killing in the presence of OGA. More important, OGA treatment intensified susceptibilities of metallo-β-lactamase-expressing Gram-negative bacteria (E. coli and Klebsiella pneumoniae) to carbapenem. Structural analyses showed that alkyl gallate bound to the binding site for acetyl-CoA that limits access of acetyl-CoA to the active site. Our data thus suggest that CysE inhibitors may be used to treat infectious diseases caused by drug-resistant Gram-negative bacteria not only via direct antibacterial activity but also by enhancing therapeutic potentials of existing antibiotics.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%