Fomes fomentarius Ethanol Extract Exerts Inhibition of Cell Growth and Motility Induction of Apoptosis via Targeting AKT in Human Breast Cancer MDA-MB-231 Cells

Lee, Seon-Ok; Lee, Min-ho; Lee, Kyung-Ran; Lee, Eun-Ok; Lee, Hyo‐Jeong

doi:10.3390/ijms20051147

Cited by 16 publications

(7 citation statements)

References 31 publications

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“…β-Thujaplicin is a natural monoterpenoid that can induce G0/G1 phase cell-cycle arrest, as well as regulate cell-cycle mediators, cyclin D1, cyclin E, and CDK4, thereby inhibiting the proliferation of ER-basal-like MCF10DCIS.com human BC cells [156]. The Fomes fomentarius ethanol extract (FFE) arrests the S and G2/M cell populations by inhibiting the expression of cell-cycle regulatory proteins, such as CDK2, cyclin A/E, and S-phase kinase-associated protein 2 (Skp2), leading to apoptosis via targeting AKT and a reduction in the migration of MDA-MB-231 cells [157]. Recently, researchers found that a new water soluble bis(hydroxymethyl) alkanoate curcuminoid derivative, MTH-3, participates in G2/M phase arrest in MDA-MB-231 cells by downregulating the expression of CDK1 [158].…”

Section: The Novel Cdk Inhibitors In Bcmentioning

confidence: 99%

The Roles of Cyclin-Dependent Kinases in Cell-Cycle Progression and Therapeutic Strategies in Human Breast Cancer

Ding

Cao

Lin

et al. 2020

IJMS

295

216

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Cyclin-dependent kinases (CDKs) are serine/threonine kinases whose catalytic activities are regulated by interactions with cyclins and CDK inhibitors (CKIs). CDKs are key regulatory enzymes involved in cell proliferation through regulating cell-cycle checkpoints and transcriptional events in response to extracellular and intracellular signals. Not surprisingly, the dysregulation of CDKs is a hallmark of cancers, and inhibition of specific members is considered an attractive target in cancer therapy. In breast cancer (BC), dual CDK4/6 inhibitors, palbociclib, ribociclib, and abemaciclib, combined with other agents, were approved by the Food and Drug Administration (FDA) recently for the treatment of hormone receptor positive (HR+) advanced or metastatic breast cancer (A/MBC), as well as other sub-types of breast cancer. Furthermore, ongoing studies identified more selective CDK inhibitors as promising clinical targets. In this review, we focus on the roles of CDKs in driving cell-cycle progression, cell-cycle checkpoints, and transcriptional regulation, a highlight of dysregulated CDK activation in BC. We also discuss the most relevant CDK inhibitors currently in clinical BC trials, with special emphasis on CDK4/6 inhibitors used for the treatment of estrogen receptor-positive (ER+)/human epidermal growth factor 2-negative (HER2−) M/ABC patients, as well as more emerging precise therapeutic strategies, such as combination therapies and microRNA (miRNA) therapy.

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Section: The Novel Cdk Inhibitors In Bcmentioning

confidence: 99%

The Roles of Cyclin-Dependent Kinases in Cell-Cycle Progression and Therapeutic Strategies in Human Breast Cancer

Ding

Cao

Lin

et al. 2020

IJMS

295

216

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“…Fomes fomentarius has diverse medicinal applications including anti-bacterial, anti-diabetes, anti-inflammatory and antioxidant activities [ 39 , 40 ]. As dry milling produces a fine powder which can be stored for months and also easily transported, practical applications of these materials are not farfetched, for instance as wound dressings, antioxidant food supplements, agricultural fertilizers and plant protectants, cosmetics, sunscreens, and possibly even as materials, for instance as redox filters in the treatment of oxidizing waste ( Figure 9 ).…”

Section: Discussionmentioning

confidence: 99%

Unleashing the Biological Potential of Fomes fomentarius via Dry and Wet Milling

et al. 2021

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Fomes fomentarius, usually referred to as tinder conk, is a common wood-based fungus rich in many interesting phytochemicals and with an unique porous structure. Dry or wet ball milling of this sponge on a planetary mill results in small particles with sizes in the range of 10 µm or below. Suspended in water and without preservatives or other stabilizers, the resulting micro-suspensions are sterile for around six days, probably due to the increased temperatures of around 80 °C especially during the wet milling process. The suspensions also exhibit excellent antioxidant activities as determined in the DPPH, ferric reducing antioxidant potential (FRAP) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. In the DPPH assay, IC50 values of 0.02—0.04% w/v and 0.04% w/v were observed for dry and wet milled samples, respectively. In the FRAP assay, IC50 values of <0.02% w/v and 0.04% w/v were observed for dry and wet milled samples, respectively. In contrast, the ABTS assay provided IC50 values of 0.04% w/v and 0.005% w/v, respectively. Notably, this activity is mostly—albeit not exclusively—associated with the highly porous particles and their large surfaces, although some active ingredients also diffuse into the surrounding aqueous medium. Such suspensions of natural particles carrying otherwise insoluble antioxidants on their surfaces provide an interesting avenue to unleash the antioxidant potential of materials such as sponges and barks. As dry milling also enables longer storage and transport, applications in the fields of medicine, nutrition, agriculture, materials and cosmetics are feasible.

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“…Betulin in the extract inhibited p-AKT in MDA-MB-231 cells. Thus, the phosphoinositide 3kinase/AKT pathway was suppressed (Lee et al, 2019b).…”

Section: Fomes Fomentariusmentioning

confidence: 99%

Mushroom extracts and compounds with suppressive action on breast cancer: evidence from studies using cultured cancer cells, tumor-bearing animals, and clinical trials

Wong

Chan

et al. 2020

Appl Microbiol Biotechnol

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This article reviews mushrooms with anti-breast cancer activity. The mushrooms covered which are better known include the following: button mushroom Agaricus bisporus, Brazilian mushroom Agaricus blazei, Amauroderma rugosum, stout camphor fungus Antrodia camphorata, Jew's ear (black) fungus or black wood ear fungus Auricularia auricula-judae, reishi mushroom or Lingzhi Ganoderma lucidum, Ganoderma sinense, maitake mushroom or sheep's head mushroom Grifola frondosa, lion's mane mushroom or monkey head mushroom Hericium erinaceum, brown beech mushroom Hypsizigus marmoreus, sulfur polypore mushroom Laetiporus sulphureus, Lentinula edodes (shiitake mushroom), Phellinus linteus (Japanese "meshimakobu," Chinese "song gen," Korean "sanghwang," American "black hoof mushroom"), abalone mushroom Pleurotus abalonus, king oyster mushroom Pleurotus eryngii, oyster mushroom Pleurotus ostreatus, tuckahoe or Fu Ling Poria cocos, and split gill mushroom Schizophyllum commune. Antineoplastic effectiveness in human clinical trials and mechanism of anticancer action have been reported for Antrodia camphorata, Cordyceps sinensis, Coriolus versicolor, Ganoderma lucidum, Grifola frondosa, and Lentinula edodes. Agaricus placomycesThe 68-kDa laccase from Agaricus placomyces belongs to a family of copper-containing oxidases involved in lignin degradation and possessing anticancer activity (Mizerska-Dudka et al. 2015). It potently expressed antiproliferative activity toward MCF-7 human breast cancer cells with an IC50 of 1.8 μM (Sun et al. 2012). Agaricus sylvaticusIn a randomized, double-blind, and placebo-controlled clinical trial, 46 patients with stage II and III breast cancer who received chemotherapy demonstrated an improved appetite and fewer gastrointestinal complaints, nausea, and vomiting following dietary supplementation with A. sylvaticus (2.1 g/day) (Valadares et al. 2013). Amauroderma rude Amauroderma rude had higher tumoricidal activity than Ganoderma lucidum. A. rude lowered cell survival and triggered apoptosis in MDA-MB-231 breast cancer cells. Tumor growth in athymic nude mice bearing MDA-MB-231-xenografts was retarded and tumor cell death was promoted by treatment with A. rude. Expression of the c-Myc oncogene was downregulated . A. rude exhibited a higher content of ergosterol (ergosta-5,7,22-trien-3β-ol) in comparison with 12 other mushrooms examined. Under the influence of ergosterol, the viability of MDA-MB-231 breast cancer cells was reduced, whereas expression of the tumor suppressor Foxo3 and downstream signaling molecules including BimL, BimS, Fas, and FasL was enhanced resulting in apoptosis of the breast cancer cells (Li et al. 2015).Amauroderma rugosum (also known as black Lingzhi, a member of five-color Lingzhi) Organic molecules from A. rugosum inhibited oxidant activity, tumor necrosis factor-α and nitric oxide formation, and MCF7 cell proliferation (Zhang et al. 2017a). A single treatment with pulverized A. rugosum (2000 mg/kg) by mouth did not impair growth, blood parameters, histological structures of t...

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Fomes fomentarius Ethanol Extract Exerts Inhibition of Cell Growth and Motility Induction of Apoptosis via Targeting AKT in Human Breast Cancer MDA-MB-231 Cells

Cited by 16 publications

References 31 publications

The Roles of Cyclin-Dependent Kinases in Cell-Cycle Progression and Therapeutic Strategies in Human Breast Cancer

The Roles of Cyclin-Dependent Kinases in Cell-Cycle Progression and Therapeutic Strategies in Human Breast Cancer

Unleashing the Biological Potential of Fomes fomentarius via Dry and Wet Milling

Mushroom extracts and compounds with suppressive action on breast cancer: evidence from studies using cultured cancer cells, tumor-bearing animals, and clinical trials

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