Following passage through the oviduct, the coelomic envelope ofDiscoglossus pictus (amphibia) acquires fertilizability upon reorganization, conversion of gp 42 to gp 40, extensive glycosylation, and formation of a specific layer

Caputo, Mariangela; Infante, Vincenzo; Talevi, Riccardo; Vaccaro, Maria Carmen; Carotenuto, Rosa; Campanella, Chiara

doi:10.1002/1098-2795(200103)58:3<318::aid-mrd10>3.0.co;2-8

Cited by 11 publications

(13 citation statements)

References 51 publications

(73 reference statements)

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“…Superposed sheets or bundles of fibrils were observed in teleost inner zona radiata [36,37], in Xenopus laevis [14] and Discoglossus pictus [13] egg envelopes. Moreover, in echinoderms, the oocyte envelope also looks threaded like a tennis ball framework [38].…”

Section: Texturementioning

confidence: 99%

“…Does a common composition induce a similar structure of egg envelopes? The answer was readily given for several groups of vertebrates by electron microscope observations: in fishes and amphibians, e.g., the main part of the envelopes appear to be made of fibril bundles or sheets distributed in superposed layers [1,13,14]. Similar observations are lacking for mammals where "it would be of great interest... to compare the high-resolution structures of vitelline envelope and ZP glycoproteins" [15].…”

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confidence: 99%

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Texture of the zona pellucida of the mature pig oocyte. The mammalian egg envelope revisited

et al. 2004

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-The zona pellucida (ZP) of mature pig oocytes is believed to consist of a dense filamentous meshwork, less compact on the inner and outer faces. The uneven surface of the ZP is made of unordered and stretched fibrils surrounding deep funnels which are the openings of the radial canaliculi. The topography of the ZP surface may contribute to the initial interplay between male and female gametes. Using cytochemical techniques for transmission electron microscopy (TEM), such as tannic acid and ruthenium red treatments, we found that the ZP of pig oocytes was essentially made of bundles of fibrils distributed in concentric layers (except in the innermost and outer parts). A correlation appears between the dense structure of the core layer of the ZP and its texture: it is constituted of superposed layers of fibril bundles, whereas only a random meshwork is found in a very thin innermost and in the outer layer. The fascicular configuration may control the permeability of the ZP, giving its semi-rigidity and elasticity, and may facilitate sperm penetration. The liquid crystal-like design of the core layer of the ZP is similar to textures found in the the vitelline envelope (zona radiata) of other vertebrates and possibly of all the deuterostomes. Such texture is probably related to the unique ZP protein composition and to a coordinated synthesis. vitelline envelope / ultrastructural cytochemistry / glycoproteins / liquid crystal-like texture / Sus scrofa Abbreviations PVS: perivitelline space; SEM: scanning electron microscopy; TEM: transmission electron microscopy; ZP: zona pellucida.

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Section: Texturementioning

confidence: 99%

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confidence: 99%

Texture of the zona pellucida of the mature pig oocyte. The mammalian egg envelope revisited

et al. 2004

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“…In Discoglosuss , gp75 and gp63 in the vitelline envelope display adhesive properties with respect to sperm (Maturi et al . 1998; Caputo et al . 2001; Infante et al .…”

Section: Introductionmentioning

confidence: 99%

“…In Bufo, some galactose residues are exposed on the acrosomereacted sperm (Takamune 1987), and saccharides containing terminal galactose residues are involved in the sperm binding to the vitelline envelope (Omata and Katagiri 1996). In Discoglosuss, gp75 and gp63 in the vitelline envelope display adhesive properties with respect to sperm (Maturi et al 1998;Caputo et al 2001;Infante et al 2004). Sulfate groups in gp75 are also thought to be involved in sperm-binding to the vitelline envelope (Caputo et al 2005).…”

Section: Introductionmentioning

confidence: 99%

The Sperm‐surface glycoprotein, SGP, is necessary for fertilization in the frog, Xenopus laevis

et al. 2009

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To identify a molecule involved in sperm-egg plasma membrane binding at fertilization, a monoclonal antibody against a sperm-surface glycoprotein (SGP) was obtained by immunizing mice with a sperm membrane fraction of the frog, Xenopus laevis, followed by screening of the culture supernatants based on their inhibitory activity against fertilization. The fertilization of both jellied and denuded eggs was effectively inhibited by pretreatment of sperm with intact anti-SGP antibody as well as its Fab fragment, indicating that the antibody recognizes a molecule on the sperm's surface that is necessary for fertilization. On Western blots, the anti-SGP antibody recognized large molecules, with molecular masses of 65-150 kDa and minor smaller molecules with masses of 20-28 kDa in the sperm membrane vesicles. SGP was distributed over nearly the entire surface of the sperm, probably as an integral membrane protein in close association with microfilaments. More membrane vesicles containing SGP bound to the surface were found in the animal hemisphere compared with the vegetal hemisphere in unfertilized eggs, but the vesicle-binding was not observed in fertilized eggs. These results indicate that SGP mediates sperm-egg membrane binding and is responsible for the establishment of fertilization in Xenopus.

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“…The gp36 and gp39 exhibit binding activity toward spermatozoa (Omata and Katagiri,'96). Similarly, during the egg oviductal transit in Discoglossus pictus, changes in the CE molecular composition occur, gp42 and gp61 being transformed into gp40 and gp63, respectively (Caputo et al, 2001).…”

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confidence: 99%

Oviductal protease and trypsin treatment enhance sperm–envelope interaction inBufo arenarum coelomic eggs

et al. 2006

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We describe the morphological and biochemical changes in Bufo arenarum coelomic egg envelopes (CE) following passage through the oviduct. In this species, the transformation of the CE into the vitelline envelope (VE) leads to the acquisition of fertilizability and involves the cleavage of a glycoprotein component. Electrophoretic patterns indicate that a pars recta oviductal protease selectively hydrolyzes in vitro the 84 and the 55 kDa glycoproteins of the CE. During the CE to VE transformation, the relative concentrations of gp48, 42 and 39 kDa also change. In in vitro tests, sperm binding to envelope glycoprotein occurs when they are exposed to VE but not when treated with CE, and VE labeled glycoproteins bind to the head and mid piece of the sperm. The gp39 VE component has 100% identity with internal domains of the sequence deduced from ovarian cDNA for the homologous zona pellucida glycoprotein type C (ZPC) protein precursor in B. arenarum. The effects of trypsin as a substitute for oviductal protease were also examined. Trypsin selectively attacks the 84 and the 55 kDa glycoproteins without hydrolyzing other components and renders coelomic eggs fertilizable in a jelly water preparation. Therefore, trypsin can mimic in vitro the biological action of the oviductal protease. However, it does not wholly mimic the biological action of the oviduct which, in B. arenarum at least, exceeds a mere proteolytic effect. This fact was verified by the lower fertility rates and the abnormal embryo development found when trypsin-treated coelomic eggs were fertilized in vitro.

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Following passage through the oviduct, the coelomic envelope ofDiscoglossus pictus (amphibia) acquires fertilizability upon reorganization, conversion of gp 42 to gp 40, extensive glycosylation, and formation of a specific layer

Cited by 11 publications

References 51 publications

Texture of the zona pellucida of the mature pig oocyte. The mammalian egg envelope revisited

Texture of the zona pellucida of the mature pig oocyte. The mammalian egg envelope revisited

The Sperm‐surface glycoprotein, SGP, is necessary for fertilization in the frog, Xenopus laevis

Oviductal protease and trypsin treatment enhance sperm–envelope interaction inBufo arenarum coelomic eggs

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