Following embryonic stem cells, their differentiated progeny, and cell-state changes during iPS reprogramming by Raman spectroscopy

Abstract

words)To monitor cell state transition in pluripotent cells is invaluable for application and basic research. In this study, we demonstrate the pertinence of use non-invasive, label-free Raman spectroscopy to monitor and characterize the cell state transition of mouse stem cells undergoing reprogramming. Using an isogenic cell line of mouse stem cells, reprogramming from neuronal cells was performed, and we showcase a comparative analysis of single cell spectral data of the original stem cells, their neuronal progenitors, and reprogrammed cells. Neural network, regression models, and ratiometric analysis were used to discriminate the cell states and extract several important biomarkers specific to differentiation or reprogramming. Our results indicated that the Raman spectrum allowed to build a low dimensional space allowing to monitor and characterize the dynamics of cell state transition at a single cell level, scattered in heterogeneous populations. Ability of monitoring pluripotency by Raman spectroscopy, and distinguish differences between ES and reprogrammed cells is also discussed.

Raman2RNA: Live-cell label-free prediction of single-cell RNA expression profiles by Raman microscopy

“…14 ). For example, Raman bands at approximately 752cm -1 (C-C, Try, cytochrome), 1004 cm -1 (CC, Phe, Tyr), and 1445 cm -1 (CH2, lipids) contributed to predicting iPSCs-related expression profiles, which is consistent with previous research that employed single cell Raman spectra to identify mouse embryonic stem cells (ESCs) 17 ( Fig. 3k ).…”

“…Feature scores for iPSC related marker genes (y axis) along the Raman spectrum (x axis). Known Raman peaks 18 were annotated.…”

“…Feature importance scores (y axis) for marker genes of each cell type (top two rows), and for all cell types (bottom row), along the Raman spectrum (x axis). Known signals 18 are annotated in the top left panel (identical to Fig. 3k).…”

Raman Spectroscopy for Chemical Biology Research

“…44 Taking advantage of the label-free approach, intensive studies are underway for intraoperational rapid diagnosis. 45,46 Raman microscopy is also expected to describe the dynamic changes in intracellular chemical compositions during cell differentiation or reprog-ramming, which has been demonstrated using embryonic stem (ES) cells, 47,48 induced pluripotent stem (iPS) 49,50 cells, and osteoblasts (Figure 2d). 51 In these experiments, the gradual changes in the chemical composition during the modulation of cellular states were visualized based on the multivariate analysis of Raman spectra measured at different time points as the cell state changes.…”

Potential of Bone-Marrow-Derived Mesenchymal Stem Cells for Maxillofacial and Periodontal Regeneration: A Narrative Review

“…On the other hand, adult stem cells are another type of stem cells with limited differentiation capacity that are not pluripotent, but they are therefore called multipotent. Adult stem cells were only located in some organs and could differentiate into just those phenotypes found in the originating tissue [1][2][3]. One type of adult stem cells is MSCs that are involved in the growth, wound healing, and replacement of cells that are lost daily by exfoliation or in pathological conditions and can differentiate into several tissues including cartilage, bone, muscle, cardiac, and blood cells.…”