Follow‐up study of human corneal endothelial cells, photographed in vivo before enucleation and 20 years later in grafts

Vasara, Kristiina; Setälä, Kai; Ruusuvaara, Pekka

doi:10.1034/j.1600-0420.1999.770305.x

Cited by 7 publications

(5 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ruusuvaara (, ); Vasara et al. ) showed in his studies great disparities between the endothelial cell densities of the graft and the recipient cornea. After the transplantation, endothelial cell density decreased more rapidly in the graft than in the recipient cornea.…”

Section: Introductionsupporting

confidence: 56%

“…Previously, we have found clear grafts with cell densities of only 500 cells/mm 2 (Ruusuvaara & Set€ al€ a 1988a,b). Ruusuvaara (1979Ruusuvaara ( , 1980; Vasara et al 1999) showed in his studies great disparities between the endothelial cell densities of the graft and the recipient cornea. After the transplantation, endothelial cell density decreased more rapidly in the graft than in the recipient cornea.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

One hundred‐year‐old human corneal and transplanted corneal graft endothelium

Ruusuvaara

Vesti

Koskela

et al. 2015

Acta Ophthalmologica

Self Cite

View full text Add to dashboard Cite

ABSTRACT.Purpose: To investigate and analyse one hundred-year-old endothelial cells of human transplanted corneal grafts and compare them with intact endothelium of unoperated eyes. Subjects and methods: Specular microscopy was performed on seven individuals (14 eyes) aged >100 years and on seven transplanted corneas with endothelial cells of at least 100 years old. All keratoplasties were performed by the same surgeon (PR) 25-35 years before the time of examination. As controls, 14 unoperated healthy individuals (26 eyes) of 90-100 years, 12 individuals (24 eyes) of 48-52 years, 12 students (24 eyes) of 19-24 years and 11 children (22 eyes) from 10 to 14 years of age were photographed and analysed. Results: The mean cell density in the oldest age group, over 100 years of age (average 102 years), was very good 2365 cells/mm 2 . The mean cell density in the transplanted corneas, the endothelial cells of which were over 100 years old (mean 106 years), was 923 cells/mm 2 (range 585-1800 cells/mm 2 ). Conclusion: Corneal endothelial cells may remain viable and capable to perform their function over 100 years. Donor corneas up to 70-75 years of age can be approved for transplantation, provided that the endothelial cells have been thoroughly evaluated with vital staining before the operation.

show abstract

Section: Introductionsupporting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

One hundred‐year‐old human corneal and transplanted corneal graft endothelium

Ruusuvaara

Vesti

Koskela

et al. 2015

Acta Ophthalmologica

Self Cite

View full text Add to dashboard Cite

show abstract

“…Reportedly, up to 30% of all donor corneas are discarded for excessive endothelium cell loss during storage (Means et al, 1995;Jones et al, 2009). Moreover, after corneal transplantation 48% of ECs die during the first year after corneal grafting (Ruusuvaara and Setala, 1988), a rate that exceeds 60% after 20 years (Vasara et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Corneal Endothelial Cells Are Protected from Apoptosis by Gene Therapy

et al. 2011

View full text Add to dashboard Cite

Corneal grafting is the most prevalent form of transplantation. Corneal endothelial cells (ECs), which form a monolayer of the cornea with minimal proliferative potential, are pivotal for maintenance of corneal clarity. Loss of EC viability and apoptosis leads to graft failure posttransplantation and reduces the quality of donor corneas in storage, such that up to 30% do not meet selection criteria and must be discarded. The current study investigates antiapoptotic effects of transduced mammalian Bcl-x(L) and baculoviral p35 on human ECs. Multiple apoptotic cell features are observed while inducing apoptosis either via the extrinsic (death receptor) or intrinsic (mitochondrial) apoptotic pathway. Human ECs were studied under three experimental conditions: (1) as an immortalized cell line, (2) as primary cells, and (3) in an intact cornea. Interestingly, in primary EC suspensions, Bcl-x(L) was protective against apoptosis mediated via both pathways. However, p35 was significantly more protective against apoptosis mediated via the intrinsic pathway compared with Bcl-x(L). Our results provide critical insight into the role of apoptotic pathways in the maintenance of EC viability and the efficacy with which these protective proteins exert their effect. These observations could form the basis for future applications of antiapoptotic gene therapy to corneal preservation aiming to reduce both graft failure after transplantation as well as donor corneal damage during storage.

show abstract

“…EC loss during storage is the principal reason for up to 30% of all discarded tissue by tissue banks 4,6 . After transplantation, there is considerable and progressive EC loss 7,8 ; indeed, EC failure contributes to at least 25% of all failed grafts 15 years post-transplantation 9 .…”

Section: Introductionmentioning

confidence: 99%

Anti-apoptotic gene therapy prolongs survival of corneal endothelial cells during storage

et al. 2011

View full text Add to dashboard Cite

Corneal transplantation is the most common form of grafting performed worldwide. Corneal endothelial cells (EC) form a monolayer in the posterior portion of the cornea and are essential for corneal transparency. EC loss during storage prior to transplantation is a principal reason for rendering donor tissue unsuitable for transplantation, and apoptosis has been shown to be the major contributor to EC loss during storage and after transplantation. Therefore, the potential use of anti-apoptotic gene therapy to promote both graft storage and graft survival is of major interest. The goal of this study was to transduce human donor corneas in vitro to enhance EC survival during storage conditions used in eye banking. We utilized a lentiviral vector to perform gene transfer of baculoviral p35 or mammalian Bcl-xL to corneal endothelium in different storage conditions utilizing a lentiviral vector. Our results show significantly enhanced survival and prolonged retention of physiological EC morphology in cells expressing either p35 or Bcl-xL. The clinical application of this technology could lead to a higher availability of donor tissue for transplantation, extend storage periods, and reduce graft failure after transplantation.

show abstract

Follow‐up study of human corneal endothelial cells, photographed in vivo before enucleation and 20 years later in grafts

Cited by 7 publications

References 22 publications

One hundred‐year‐old human corneal and transplanted corneal graft endothelium

One hundred‐year‐old human corneal and transplanted corneal graft endothelium

Corneal Endothelial Cells Are Protected from Apoptosis by Gene Therapy

Anti-apoptotic gene therapy prolongs survival of corneal endothelial cells during storage

Contact Info

Product

Resources

About