Follow-Up of Natural Products Isolation

Cannell, Richard J. P.; Sarker, Satyajit D.; Nahar, Lutfun

doi:10.1007/978-1-61779-624-1_19

Cited by 14 publications

(17 citation statements)

References 67 publications

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“…Purified marine-natural compound is mandatory for structural characterization, and to identify its biological and chemical activities. In some cases, certain structural feature was not detected in a crud extract, which indicates that the extract does not contain the desired compound(s) [108]. Fractionation process mainly conducted through the separation of a crude mixture into a several number of separate fractions.…”

Section: Purity and Fractionationmentioning

confidence: 99%

Red Sea Microbial Diversity for Antimicrobial and Anticancer Agents

M¹

2016

J Mol Biomark Diagn

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Marine natural products are valuable sources that could produce potential chemotherapeutic agents, and marinederived organisms are widely recognized as important sources of these natural products with interesting biological activities. Furthermore, Red sea was recognized as a rich source of microbial diversity with unique metabolites that can be of pharmaceutical and medicinal importance. The discovery of novel active metabolites provides an intellectual challenge to examine new products for medicinal purposes. Although many bacterial strains were isolated from Red sea with potent bioactivities; however, the microbial diversity of marine environment such Red sea remained largely un-explored. Lately, it has been possible to easily collect marine samples, isolate, and further identify diverse marine organisms (e.g. bacteria, fungi, and algae and marine invertebrate) by modern molecular techniques. Based on a wide range of screening and extraction methods, a vast number of metabolites have been extracted from marine sources with potential biological activities such as antibacterial, anticancer, anti-inflammatory agents and many others. In this article, we have reviewed the previous studies that have been conducted to explore marine-derived bioactives of Red sea. In addition, we have illustrated the following aspects: (i) Red sea microbial diversity for antibacterial and anticancer agents; (ii) the conventional and molecular approach used for screening and characterization marine microorganisms; (iii) characterization of natural products of microbial origin, and (iv) drug development from novel marine-derived metabolites and clinical trials. Moreover, modern molecular technologies, meta-genomic, proteomic and bio-chemo-informatics approaches are adding more potency to the targeted search of bioactive compounds derived from marine microbes. The novel marine derived drug discovery is aimed to improve our understanding of natural resources, to improve public health awareness about the environmental microbial diversity and their importance for nutraceuticals, pharmaceutical, agrochemicals and food processing. Recently, the research area of microbial diversity of the Red sea and their metabolites has opened a new access for significant sources of more effective drug entities with low coast.extreme conditions such as high temperature, pressure, and salinity. The experimental results suggested that these bacterial and archaeal communities are rich sources of active enzymes and metabolites, which can be applied for pharmaceutical and biotechnology-based applications [9].In previous studies, a number of bio-active compounds have been reported from marine sources with different degrees of action and potential, such as antibacterial, anticancer, antitumor, antiproliferative, anti-microtubule, cytotoxic, photoprotective, and antifouling properties [10][11][12]. Marine-derived metabolites, like peptides (ribosomal and non-ribosomal), alkaloids, polyketides and terpenes, have demonstrated antimicrobial and antiviral activ...

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Section: Purity and Fractionationmentioning

confidence: 99%

Red Sea Microbial Diversity for Antimicrobial and Anticancer Agents

M¹

2016

J Mol Biomark Diagn

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“…The dried collection was designated as "purified Myristicin". The liquid-adsorption technique was suggested by [39]. …”

Section: Myristicin Purificationmentioning

confidence: 99%

Extraction and Purification of Terpenes from Nutmeg (myristica fragrans)

Al-Jumaily

Al-Amiry

2012

JNUS

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This study was conducted with the aim to extract and purify the essential oil containing terpenes from the dried seeds of nutmeg myristica fragrans available in Iraqi markets.The essential oil was extracted by steam distillation method with a yield of 4.7 -7.5 gm / 100 gm, and the resultant oil was subjected to sensory and physical evaluation. It has a special spicy odor, slightly pungent taste, colorless to pale yellow in color, not dissolved in water but dissolved in organic solvents like ethanol, hexane, chloroform and ether. The specific gravity, refractive index and optical rotation were (0.890 gm / ml), (1.4822) and (+22˚), respectively. Gas chromatography is used for the quality and quantity evaluation of the essential oil constituents which revealed the presence of 49 volatile compounds. The methanolic extract obtained by reflux the seeds powder with 70% methanol in soxhlet apparatus and yield 12.8%. The extract was subjected to phytochemical detection which confirms the presense of alkaloids, terpenes, flavonoids, glycosides, tannins and resins, with the exception of saponins and coumarines which gave negative tests. Myristicin was purified from the methanolic extract of nutmeg dried seeds, which were detected in the essential oil by TLC and vanillin-H2SO4 reagent. The purified myristicin was obtained after the application of adsorption chromatography on silica gel column and detected on T.L.C. plate with standard myristicin.Both the essential oil and purified myristicin were subjected to gas chromatography which showed the presence of myristicin in the oil in a concentration of 6%.

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“…The methanolic extract was dried using rota-evap apparatus (yield 13.9% w/w). The qualitative phytochemical screenings of plant extract was also carried out to investigate the presence of class of constituents e.g., alkaloids, flavonoids, glycosides, tannins, phenols and steroids (Cannell, 1998).…”

Section: Collection Extraction and Phytochemical Screening Of Plant mentioning

confidence: 99%

POSSIBLE MECHANISM OF <i>PLUMBAGO ZEYLANICA</i> IN PREVENTION OF HEPATIC DAMMAGE IN WISTAR RAT

Goyal¹

2012

American Journal of Pharmacology and Toxicology

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Plumbago zeylanica root has been used traditionally to treat various body ailments including liver diseases. The present study was designed to investigate the hepatoprotective effects of standardized methanolic extract of Plumbago zeylanica root on paracetamol, CCl 4 and alcohol induced hepatic injuries in rats and its possible mechanism(s) of hepatoprotection. Administration of hepatotoxicants like paracetamol (3 g kgto Wistar albino rats (180-240 g of either sex, n = 8) caused significant increase in serum ALT, AST, ALP and bilirubin; tissue lipid peroxidation, nitrite/nitrate, collagen; and decrease in tissue GSH and SOD levels. The liver histopathology revealed central vein dilation, infiltration and fatty degeneration in hepatotoxicant control groups. Pretreatment with methanolic extract of Plumbago zeylanica (100, 200 and 400 mg kg −1 ) and silymarin (50 mg kg −1 ) as standard significantly reversed these toxic changes dose dependently, as compared to hepatotoxicant control. The results in present study suggested that Plumbago zeylanica root possesses marked hepatoprotective potential through its oxidative, inflammatory and fibrotic effects against experimentally induced liver toxicity. These findings support and extend the rational basis for the use of this plant as hepatoprotective in traditional and folk-lore medicine.

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Follow-Up of Natural Products Isolation

Cited by 14 publications

References 67 publications

Red Sea Microbial Diversity for Antimicrobial and Anticancer Agents

Red Sea Microbial Diversity for Antimicrobial and Anticancer Agents

Extraction and Purification of Terpenes from Nutmeg (myristica fragrans)

POSSIBLE MECHANISM OF <i>PLUMBAGO ZEYLANICA</i> IN PREVENTION OF HEPATIC DAMMAGE IN WISTAR RAT

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